Preparation of Quantum Dot Bioconjugates as Fluorescence Probe for Targeting and Visualization of Cells

Year 2017, Volume: 45 Issue: 3, 417 - 423, 01.09.2017

Dilek Odaci Demirkol Suna Timur

Abstract

Here, the bioconjugation and application of water-soluble quantum dots QDs for targeted cellular imaging were investigated. Human Anti-c-ErbB2 antibodies were bound to QDs for the targeting of these nanostructures towards the cancer cells. QDs and Anti-c-ErbB2/QD bioconjugates were characterized by fluorescence and UV–Vis spectroscopy and hydrodynamic sizing. The effect of QDs and Anti-c-ErbB2/QD bioconjugates on cell viability was evaluated using an MTT assay. To observe targeting efficiency of Anti-c- ErbB2/QD bioconjugates, fluorescence microscopy and was performed. Higher cell affinities were obtained by Anti-c-ErbB2-conjugated QDs which is an evidence that these are well-suited for targeting and imaging of cells.

Keywords

Quantum dots, bioconjugation, anti-c-ErbB2, cell targeting

Hücrelerin Hedeflenmesi ve Görüntülenmesi için Floresans Prob Olarak Kuantum Nokta Biyokonjugatlarının Hazırlanması

Abstract

B urada, hedeflenmiş hücresel görüntüleme için suda çözünür kuantum noktaların QD biyokonjugasyonu ve uygulamaları gerçekleştirilmiştir. Kanser hücrelerine nanoyapıların hedeflenmesi için anti-c-ErbB2 antikoru QD’lara bağlanmıştır. QD’lar ve anti-c-ErbB2/QD biyokonjugatları floresans/UV-Görünür bölge spektroskopisi ve hidrodinamik boyut ölçümleri ile karakterize edilmiştir. Hücre canlılığına QD’lar ve antic-ErbB2/QD biyokonjugatlarının etkisi MTT denemeleri kullanılarak gerçekleştirilmiştir. Anti-c-ErbB2/QD biyokonjugatlarının hedefleme etkinliğini gözlemlemek için, floresans mikroskopi kullanılmıştır. Hücreleri görüntüleme ve iyi gerçekleştirilmiş hedeflemenin ispatı olarak anti-c-ErbB2 konjuge edilen QD’lar ile daha yüksek hücre afiniteleri elde edilmiştir

Keywords

Kuantum noktalar, biyokonjugasyon, anti-c-ErbB2, hücre hedefleme

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