Microporous Poly hydroxyethyl methacrylate based Affinity Membranes Carrying Heparin for Cholesterol Apheresis

Year 2009, Volume: 37 Issue: 4, 271 - 280, 01.12.2009

Lokman Uzun Handan Yavuz Veyis Karakoç Hamdi Çelik Adil Denizli

Abstract

In this study, the preparation of polymeric membranes using bioaffinity technology for application in blood filtration devices is described. Low molecular weight heparin LMWH attached poly hydroxyethyl methacrylate PHEMA based microporous affinity membranes were used for selective removal of cholesterol from hypercholesterolemic human plasma in-vitro. In order to further increase blood-compatibility of affinity membranes, aminoacid based comonomer N-methacryloyl-L-alanine MAAL was included in the polymerization recipe. PHEMAAL membranes were produced by a photopolymerization technique and then characterized by swelling test and scanning electron microscope SEM studies. In vitro blood-compatibility tests were assessed using modified activated partial thromboplastin time APTT , prothrombin time PT and coagulation time CT . The water swelling ratio of PHEMAAL increased significantly 133.2% compared with PHEMA 58% . PHEMAAL membranes have large pores around in the range of 5-10 µm. All the clotting times increased when compared with PHEMA membranes. Loss of platelets and leukocytes was very low. LMWH loading amount was in the range of 87.5-147.2 mg/g. There was a very low non-specific cholesterol adsorption onto the PHEMA membranes, about 0.69 mg/g. LMWH attached PHEMAAL membranes adsorbed cholesterol in the range of 12.7-22.6 mg/g from hypercholesterolemic human plasma.

Keywords

PHEMA, Cholesterol removal, Affinity membranes, Hypercholesterolemia, Apheresis, Heparin

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