Scanning of Some Herbal Tissues to be Used with Biosensors as Polyphenol Oxidase Enzyme Source

Year 2009, Volume: 37 Issue: 4, 303 - 312, 01.12.2009

Ayten Sağıroglu Hakkı Mevlüt Özcan Özhan Hasancebi

Abstract

The purpose of this study is to examine levels of polyphenol oxidase PPO activities in the crude extracts of seventeen different herbal tissues Banana peel, Jerusalem artichoke, black grape, buckthorn, fresh bean, pea, cactus apple, aloe vera, cabbage turnip graim, cabbage turnip vegetable, celeriac, quince, artichoke, aubergine, Trabzon palm, dry carob with spectrophotometric method. The three herbal tissues Anamur banana peel, Jerusalem artichoke and fresh broad bean were found to have higher PPO activities than other tissues, 638, 3360, 3677 U/min.mg protein, respectively. Biologic oxygen biosensors were prepared by cross-linked thin film immobilization method, in the presence of gelatin and glutaraldehyde, with three herbal tissues used as biomaterials and these biosensors were used for detection of phenolic compounds via determining the concentration of consumed oxygen throughout the reaction medium. The typical calibration curves for the Anamur banana peel, Jerusalem artichoke and fresh broad bean based sensors revealed a linear range of 5-40 μM, 2-10 μM, 10-100 μM, respectively. In reproducibility studies, variation coefficiets CV and standard deviations SD were calculated as 1.4%-0.3294 μM, 2%-0.8392 μM and 1%-0.0746 μM, respectively.

Keywords

biosensor, Herbal tissue, Phenolic compound, Consumption oxygen

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