Preparation of bacterial cellulose/vinyl imidazole-based membranes for selective purification of Hemoglobin

Year 2021, Issue: 4, 423 - 431, 25.05.2021

Monireh Bakhshpour , Adil Denizli

https://doi.org/10.15671/hjbc.921540

Cited By: 1

Abstract

The development of new biomaterials is needed to use in many areas such as protein purification, removal and drug release system. In this study, we fabricated hemoglobin (Hb) surface imprinting onto the bacterial cellulose nanofibers. The metal ions coordination was obtained with vinyl imidazole and Ni2+ ions. The selective purification of Hb was obtained using Hb-imprinted bacterial cellulose. The Scanning Electron Microscopy, Surface area, Transformed Infrared Spectroscopy, swelling tests and contact angle measurements were used for the characterization of Hb-imprinted and non-imprinted bacterial cellulose membranes. In addition, the adsorption studies were experimented with using the Hb aqueous solution in the batch systems at 2 h. In this study, the prepared bacterial cellulose was reported unique biomaterials for selective and sensitive purification of Hb with high adsorption capacity. The reusability of bacterial celluloses was demonstrated during the experiment.

Keywords

Purification, ions coordination, bacterial cellulose, hemoglobin.

References

• 1. Z. Liu,Y.Wang, F. Xu, X. Wei, J. Chen, H. Li, X. He, Y. Zhou, A new magnetic molecularly imprinted polymer based on deep eutectic solvents as functional monomer and cross-linker for specific recognition of bovine hemoglobin, Anal. Chim. Acta. 1129 (2020) 49-59.
• 2. S. Diken Gür, M. Bakhshpour, N. Bereli, A. Denizli, Antibacterial effect against both Gram-positive and Gram-negative bacteria via lysozyme imprinted cryogel membranes, J. Biomater. Sci. Polym. Ed. 0 (2021) 1–16.
• 3. N. Bereli, H. Yavuz, A. Denizli, Protein chromatography by molecular imprinted cryogels, J. Liq. Chromatogr. Relat. Technol. 43 (2020) 657-670.
• 4. J. Kalecki, Z. Iskierko, M. Cieplak, P.S. Sharma, Oriented Immobilization of Protein Templates: A New Trend in Surface Imprinting, ACS Sensors. 5 (2020) 3710–3720.
• 5. R. Xing, Y. Ma, Y. Wang, Y. Wen, Z. Liu, Specific recognition of proteins and peptides via controllable oriented surface imprinting of boronate affinity-anchored epitopes, Chem. Sci. 10 (2019) 1831–1835.
• 6. S. Gorgieva, J. Trček, Bacterial cellulose: Production, modification and perspectives in biomedical applications, Nanomaterials. 9 (2019) 1–20.
• 7. G.F. Picheth, C.L. Pirich, M.R. Sierakowski, M.A. Woehl, C.N. Sakakibara, C.F. de Souza, A.A. Martin, R. da Silva, R.A. de Freitas, Bacterial cellulose in biomedical applications: A review, Int. J. Biol. Macromol. 104 (2017) 97–106.
• 8. F.G. Torres, J.J. Arroyo, O.P. Troncoso, Bacterial cellulose nanocomposites: An all-nano type of material, Mater. Sci. Eng. C. 98 (2019) 1277–1293.
• 9. M. Moniri, A.B. Moghaddam, S. Azizi, R.A. Rahim, A. Bin Ariff, W.Z. Saad, M. Navaderi, R. Mohamad, Production and status of bacterial cellulose in biomedical engineering, Nanomaterials. 7 (2017) 1–26.
• 10. B. V. Mohite, S. V. Patil, A novel biomaterial: Bacterial cellulose and its new era applications, Biotechnol. Appl. Biochem. 61 (2014) 101–110.
• 11. C., Buruaga-Ramiro, S.V., Valenzuela, C. Valls, et al. Bacterial cellulose matrices to develop enzymatically active paper. Cellulose 27 (2020) 3413–3426.
• 12. Y. Saylan, E. Tamahkar, A. Denizli, Recognition of lysozyme using surface imprinted bacterial cellulose nanofibers, J. Biomater. Sci. Polym. Ed. 28 (2017) 1950-1965.
• 13. E. Tamahkar, M. Bakhshpour, A. Denizli, Molecularly imprinted composite bacterial cellulose nanofibers for antibiotic release, J. Biomater. Sci. Polym. Ed. 30 (2019) 450–461.
• 14. Y. Saylan, llgım Göktürk, K. Pospiskova, I. Safarik, A. Denizli, Magnetic bacterial cellulose nanofibers for nucleoside recognition, Cellulose. 27 (2020) 9479–9492.
• 15. M. Bakhshpour, E. Tamahkar, M. Andaç, A. Denizli, Surface imprinted bacterial cellulose nanofibers for hemoglobin purification, Colloids Surfaces B Biointerfaces. 158 (2017) 453–459.
• 16. M. Liu, S. Li, Y. Xie, S. Jia, Y. Hou, Y. Zou, C. Zhong, Enhanced bacterial cellulose production by Gluconacetobacter xylinus via expression of Vitreoscilla hemoglobin and oxygen tension regulation, Appl. Microbiol. Biotechnol. 102 (2018) 1155–1165.
• 17. H. Bagán, T. Zhou, N.L. Eriksson, L. Bülow, L. Ye, Synthesis and characterization of epitope-imprinted polymers for purification of human hemoglobin, RSC Adv. 7 (2017) 41705–41712.
• 18. K. Zhang, T. Zhou, K. Kettisen, L. Ye, L. Bülow, Chromatographic separation of hemoglobin variants using robust molecularly imprinted polymers, Talanta. 199 (2019) 27–31.
• 19. J. Elmer, D. Harris, A.F. Palmer, Purification of hemoglobin from red blood cells using tangential flow filtration and immobilized metal ion affinity chromatography, J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 879 (2011) 131–138.
• 20. H. Yavuz, V. Karakoç, D. Türkmen, R. Say, A. Denizli, Synthesis of cholesterol imprinted polymeric particles, Int. J. Biol. Macromol. 41 (2007) 8–15.
• 21. N.F., Vasconcelos, F.K., Andrade, L.d.A.P. Vieira, et al. Oxidized bacterial cellulose membrane as support for enzyme immobilization: properties and morphological features. Cellulose 27 (2020) 3055–3083.
• 22. B. Demirci, N. Bereli, S. Aslıyüce, G. Baydemir, A. Denizli, Protein C recognition by ion-coordinated imprinted monolithic cryogels, J. Sep. Sci. 40 (2017) 1610–1620.
• 23. S. Mallik, R.D. Johnson, F.H. Arnold, Selective Recognition of Bis-Imidazoles by Complementary Bis-Metal Ion Complexes, J. Am. Chem. Soc. 115 (1993) 2518–2520.
• 24. M. Bakhshpour, E. Tamahkar, M. Andaç, A. Denizli, Affinity binding of proteins to the modified bacterial cellulose nanofibers, J. Chromatogr. B. 1052 2017 (2017) 121-127.
• 25. S. Akgönüllü, M. Bakhshpour, N. İdil, M. Andaç, H. Yavuz, A. Denizli, Versatile polymeric cryogels and their biomedical applications, Hacettepe J. Biol. Chem. 48 (2020) 99-118.