Establishing an Oxidative Stress Model in the Human Mesencephalic Cell Line (LUHMES): an in vitro study

Year 2024, Volume: 52 Issue: 2, 77 - 83, 01.04.2024

Handan Sevim Akan , Özgü Zuğa Örsoğlu , Özer Aylin Gurpinar

https://doi.org/10.15671/hjbc.1374824

Abstract

Oxidative stress-caused neurodegenerative diseases, such as Alzheimer's, Parkinson's disease, and amyotrophic lateral sclerosis, are widely recognized as the most prevalent brain and central nervous system disorders. This is attributed to the vulnerability of neurons to oxidative stress within the body. Although substantial research has been performed on these diseases, it is extremely difficult to establish an oxidative stress model for brain tissues. In primary cultures, it is difficult to obtain neurons and the continuity of the culture is limited for in vitro cell line models. By providing valuable insights into the mechanisms of oxidative stress-induced neurodegenerative diseases, these in vitro models can aid in the development of effective treatment strategies. Here, we developed an in vitro oxidative stress model utilizing hydrogen peroxide on the LUHMES cell line. Our study evaluated the impact of this model on LUHMES cell viability and the equilibrium between oxidants and antioxidants by assaying total oxidant capacity (TOC) and total antioxidant capacity (TAC). Our results provided evidence of the oxidative effect of hydrogen peroxide in critical concentration and proved the efficacy of this model for further investigations.

Keywords

Oxidative stress, LUHMES cell line, total oxidant capacity, total antioxidant capacity

Ethical Statement

No ethical statement needed.

Supporting Institution

This work was supported by grants from Hacettepe University Scientific Research Projects Coordination Unit (Grand number: FHD.21.19514).

Project Number

FHD.21.19514

Thanks

This work was supported by grants from Hacettepe University Scientific Research Projects Coordination Unit (Grand number: FHD.21.19514).

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