Serum Levels of IL-21, IL-23 and 8-hydroxy-2′-deoxyguanosine in Pediatric Severe Pneumonia Cases

Abstract

Background: Pneumonia causes the majority of acute respiratory distress syndrome (ARDS) cases. The microbes that cause pneumonia are very diverse. In addition to DNA, RNA viruses, Gram-negative and Gram-positive bacteria cause two types of cytokine imbalances, anti-inflammatory and pro-inflammatory. It can also influence the progno-sis of sepsis and other infectious diseases. This study aims to search for 8-hydroxy-2'-deoxyguanosine (8-OHdG), IL-21, IL-23, and c-reactive protein (CRP) and compare cytokine levels. It is also to determine if Pediatric pneumonia patients CRP and cytokine levels correlate with results. Materials and Methods: In the study, blood was drawn from approximately 43 pediatric pneumonia patients and 43 healthy controls who came to the pediatric clinic to investigate serum IL-21, IL-23, 8-OHdG, and CRP levels. The levels of biomarkers were determined by ELISA method. Serum CRP levels were measured using the ATELLICA IM Analyzer. Results: Serum CRP, 8-OHdG, IL-21 and IL-23 levels were significantly higher in the pediatric pneumonia patient group than in the control group. Conclusions: Increased serum IL-21, IL-23, 8-OHdG and CRP expression in pediatric pneumonia patients is a poten-tial determinant suggesting that IL-21, IL-23-related cytokines may play a role in endothelial cell activation reported in patients. Increased 8-OHdG oxidative stress is more pronounced in patients without pediatric pneumonia while pro inflammatory cytokines are higher in pediatric pneumonia patients. However, it is used as a possible therapeu-tic target to reduce inflammation. Further study on the impact of these findings on comorbidities with larger num-ber test size is needed

Keywords

• Pneumonia
• IL-21
• IL-23
• 8-OHdG

Pediatrik Şiddetli Pnömoni Vakalarında IL-21, IL-23 ve 8-hidroksi-2'-deoksiguanozin Serum Düzeyleri

Abstract

Objective: Pneumonia causes the majority of acute respiratory distress syndrome (ARDS) cases. The microbes that cause pneumonia are very diverse. In addition, RNA viruses, DNA viruses, enveloped viruses, non-enveloped viruses, Gram positive and negative bacteria affect the imbalance in two types of cytokines, pro inflammatory and anti-inflammatory, and may affect the prognosis of sepsis and other infectious and inflammatory diseases. The aim of the study was to compare clinical features and cytokine levels in young patients with severe pneumonia who require investigation of IL-21, IL-23, 8-hydroxy-2′-deoxyguanosine (8-OHdG) and c-reactive protein (CRP) levels in pediatric pneumonia patients to determine whether cytokine levels are associated with outcome in pediatric cases of severe pneumonia. Method: In this study, blood was drawn to investigate serum IL-21, IL-23, 8-OHdG and CRP levels in approximately 43 with pediatric pneumonia patients and 43 healthy controls who came to the pediatric clinic. The levels of tested Biomarkers were studied by Elisa method and CRP levels of the serum were measured using Atellica IM Analyzer. Results: Serum IL-21, IL-23, 8-OHdG and CRP levels were significantly higher in pediatric pneumonia patients group compared to the control group. Statistically significant differences were observed between the groups. Conclusion: Our results showing increased serum IL-21, IL-23, 8-OHdG and CRP expression in pediatric pneumonia patients concluded that it is a potential determinant, suggesting that IL-21, IL-23-related cytokines may play a role in endothelial cell activation reported in patients. Increased 8-OHdG oxidative stress is more pronounced in patients without pediatric pneumonia while pro inflammatory cytokines are higher in pediatric pneumonia patients. Further studies on the impact of these findings on comorbidities. However, their true significance may serve as a possible therapeutic target for reducing inflammation. To test these concepts, additional preclinical trials and clinical trials with larger cohort sizes will be required.

Keywords

• Pneumonia
• IL-21
• IL-23
• 8-OHdG

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