Protective Effects of Resveratrol Carbon Dots Against 6-OHDA-Induced Neurotoxicity in SH-SY5Y Cells

Abstract

Background: We aimed to investigate the ability of resveratrol carbon dots (RES C-Dots) to protect SH-SY5Y cells from oxido-inflammatory stress and apoptosis caused by 6-hydroxydopamine (6-OHDA). Materials and Methods: In vitro PD model was generated in SH-SY5Y cells by administering of 200 µM 6-OHDA for 24 hours. Different concentrations of RES C-Dots (12.5, 25, and 50 µg/mL) were applied to the cells 30 minutes before administration of 6-OHDA. Results: We observed that application of RES C-Dots prevented cell death induced by 6-OHDA and main-tained cell viability. As expected, RES C-Dots prevented oxidative damage induced by 6-OHDA - by strengthening the total amount of antioxidants and lowering the total amount of oxidants in SH-SY5Y cells. Similarly, RES C-Dots markedly alleviated the secretion of inflammatory factors (TNF-α and IL-1β) promot-ed by 6-OHDA. Furthermore, RES C-Dots prevented apoptosis induced by 6-OHDA by suppressing caspase-3 mRNA expression level. Conclusions: RES C-Dots rescued SH-SY5Y cells from 6-OHDA- induced damage by modulating the oxido-inflammatory and apoptotic response. This report indicates enounces that RES- synthesised C-Dots may have promising curative potential for PD.

Keywords

• Parkinson Disease
• 6-OHDA
• RES C-Dots
• SH-SY5Y cells

Resveratrol Karbon Noktalarının SH-SY5Y İnsan Nöroblastoma Hücrelerinde 6-OHDA Kaynaklı Nöronal Hücre Ölümü Üzerinde Koruyucu Etkileri

Abstract

Amaç: Bu çalışmada Resveratrol karbon noktalarının (RES-KN) 6-OHDA'nın SH-SY5Y insan nöroblastoma hücrelerininde neden olduğu oksidoinflamatuar stres ve apoptozdan koruma potansiyelini araştırmayı amaçladık. Materyal ve Metod: SH-SY5Y hücreleri, in vitro PH modelini indüklemek için 24 saat boyunca 200 μM 6-OHDA'ya maruz bırakıldı. Hücrelere, 6-OHDA uygulamasından 30 dakika önce farklı konsantrasyonlarda RES KN (12.5, 25, and 50 µg/mL) uygulandı. Bulgular: Özellikle, RES KN uygulaması sonucu 6-OHDA'nın neden olduğu hücre ölümü etkili bir şekilde engellendiğini ve SH-SY5Y hücrelerinde hücre canlılığı önemli ölçüde korunduğunu gözlemledik. RES-KN, SH-SY5Y hücrelerinde toplam antioksidanları güçlendirerek ve toplam oksidanları düşürerek 6-OHDA kaynaklı oksidatif hasarı önledi. Benzer şekilde, RES-KN, 6-OHDA kaynaklı inflamatuvar faktörlerin (TNF-a ve IL-1β) salınımını önemli ölçüde azalttı. Ayrıca RES-KN, kaspaz-3 mRNA ekspresyonunu baskılayarak 6-OHDA’nın neden olduğu apoptozu engelledi. Sonuç: RES-KN’lar, oksido-inflamatuar ve apoptotik yanıtı modüle ederek SH-SY5Y hücrelerini 6-OHDA kaynaklı nörotoksisiteden kurtardı. Bu çalışma, RES’den sentezlenen KN’lerin PH tedavisinde umut verici terapötik potansiyele sahip olabileceğini düşündürmektedir.

Keywords

• Parkinson's disease
• 6-OHDA
• RES C Dots
• SH-SY5Y cells

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