Preeklampsi Plasentada Bax ve Fosfo-Tau Protein Ekspresyonunun Araştırılması

Abstract

Amaç: Preeklampsi, morbidite ve mortalite oranı yüksek bir gebelik komplikasyonudur. Bax proapoptotik bir proteindir ve son yıllarda 14-3-3 tau proteinleri, apoptoz gibi birçok sürecin anahtar düzenleyicileri olarak kabul edilmektedir. Bu çalışmada preeklampsi apoptozunda fosfo-tau ve Bax ekspresyonunu immünohistokimyasal olarak araştırdık. Materyal ve Metod: 25 sağlıklı ve 25 preeklamptik gebe plasenta çalışmaya dahil edildi. Plasental örnekler %10 nötr tamponlu formalinle sabitlendi. Rutin parafin mum doku protokolü kullanıldı. Bulgular: Histolojik sonuçlara göre kontrol grubunda villuslardaki trofoblastik hücreler normaldi. Ve kan damarlarında hafif genişleme görüldü. Preeklampsi kesitlerinde koryon villuslarında hyalinize ve nekrotik alanlar ve kök villuslarında fibrinoid doku artışı saptandı. İmmünohistokimyasal değerlendirme için fosfo-tau ve Bax primer antikorları kullanıldı. Preeklampsi grubundan alınan kesitler sinsityotrofoblastlar ve villöz bağ dokusu açısından oldukça pozitifti. Kontrol grubundan istatistiksel olarak da farklıydı (p<0.05). Sonuç: Bulguları incelediğimizde, Phospho-tau ve Bax'ın preeklampsinin apoptoz yolağında belirleyici proteinler olabilir

Keywords

• apoptoz
• bax
• fosfo-tau
• preeklampsi

Investigation of Bax and Phospho-Tau Protein Expression in Preeclampsia Placenta

Abstract

Background: Preeclampsia is a pregnancy complication with a high morbidity and mortality rate.BAX is a proapoptotic protein and in recent years, 14-3-3 tau proteins are considered as key regulators of many processes, such as apoptosis. In this study, we investigated the expression of phospho-tau and Bax in preeclampsia apoptosis immunohistochemically. Materials and Methods: 25 healthy and 25 preeclamptic pregnant placentas were included in the study. Placental samples were fixed with 10% neutral buffered formalin. Routine paraffin wax tissue protocol was used. Results: According to histological micrograf, the trophoblastic cells in the villi were normal in the control group. Mild dilatation in blood vessels was seen. Hyalinized and necrotic areas in chorionic villi and an increase in fibrinoid tissue in root villi were detected in preeclampsia sections. Phospho-tau and Bax primary antibodys were used for immunohistochemical evaluation. The sections from of the preeclampsia group were highly positive for syncytiotrophoblasts and villous connective tissue. It was also statistically different from the control group (p<0.05). Conclusions: When we evaluate the results shows that Phospho-tau and Bax may be determinant proteins in the apoptosis pathway of preeclampsia.

Keywords

• apoptosis
• bax
• phospho-tau
• preeclampsia

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