The Investigation of Oxidative Stress Parameters in Patients with Lipoid Proteinosis

Abstract

Objective: This study aims to investigate the role of oxidative stress in pathogenesis of Lipoid Proteinosis (LP) by measuring the parameters of serum oxidative stress such as total antioxidant status (TAS), total oxidant status (TOS), advanced oxidation protein products (AOPP), oxidative stress index (OSI), ferric reducing antioxidant power (FRAP), lipid hydroperoxide (LOOH) and total free sulfhydryl groups (Total-SH).

Materials and Methods: Fourteen patients with LP diagnosed clinically, radiologically and histopathologically and 14 sex-, age- and body mass index- matched healthy volunteers were included in this prospective case-control study. Serum TAS, TOS, AOPP, FRAP, LOOH and Total-SH were measured spectrophotometrically and OSI were calculated.

Results: Cases with LP had significantly lower serum levels of TAS, FRAP and Total –SH (p< 0.001, for each) and higher levels of OSI, AOPP and LOOH (p= 0.001, p= 0.018, and p= 0.047, respectively). Serum TOS levels were higher in the LP group when compared to that of the control group, but it was not statistically significant (p= 0.081). ROC analysis demonstrated that TAS, FRAP, Total-SH and OSI levels independently predicted LP with strong sensitivity and specificity.

Conclusion: Our results demonstrate that decreased levels of antioxidant parameters and increased levels of oxidant parameters might be used as an ancillary laboratory tests in the diagnosis of LP disease.

Keywords

• Lipoid Proteinosis,Oxidative Stress,Antioxidant

Lipoid Proteinozis Hastalarında Oksidatif Stres Parametrelerinin Araştırılması

Abstract

Amaç: Bu çalışmadaki amacımız, total antioksidan seviye (TAS), ferrik iyon indirgeyici antioksidan güç (FRAP) ve toplam serbest sülfhidril grupları (Total-SH) gibi antioksidan parametreler ile total oksidan seviye (TOS), ileri oksidasyon protein ürünleri (AOPP), oksidatif stres indeksi (OSI) ve lipid hidroperoksit (LOOH) gibi oksidasyon parametrelerini ölçerek Lipoid Proteinozlu (LP) hastalarda oksidatif stresin rolünü araştırmaktır.

Materyal ve Metod: Bu prospektif vaka kontrol çalışmasına klinik, radyolojik ve histopatolojik olarak tanı konulan 14 LP hastası ve cinsiyet, yaş ve vücut kitle indeksi benzer 14 sağlıklı gönüllü katılımcı dahil edildi. Serum TAS, TOS, AOPP, FRAP, LOOH ve Total-SH seviyeleri spektrofotometrik olarak ölçüldü ve OSI değeri hesaplandı.

Bulgular: LP'li hastalarda serum TAS, FRAP ve Total –SH (p< 0,001; her biri için) seviyeleri daha yüksek bulunurken OSI, AOPP ve LOOH düzeyleri daha düşük (sırasıyla, p= 0,001, p= 0,018 ve p= 0,047) bulundu. LP hastalarında serum TOS seviyeleri kontrol grubuna göre daha yüksekti, ancak bu yükseklik istatistiksel olarak anlamlı değildi (p= 0,081). Yapılan ROC analizinde TAS, FRAP, Total-SH ve OSI seviyelerinin LP hastalığını güçlü bir duyarlılık ve yüksek bir özgüllük ile öngördüğünü gösterdi.

Sonuç: LP hastalığının tanısında, antioksidan parametrelerindeki azalma ve oksidan parametrelerindeki artmanın yardımcı laboratuvar testleri olarak kullanılabileceğini göstermektedir.

Keywords

• Lipoid Proteinozis,Oksidatif Stres,Antioksidan

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