Investigation of Methylglioxal, Ischemia-Modified Albumin Levels and Prolidase Activity in Behcet's Patients

Abstract

Background: Behçet's disease is an autoimmune, chronic, inflammatory, multisystemic disease. Although oral aphthae, genital ulcers, skin lesions and uveitis are commonly seen in the disease, vasculitis is the main pathological finding. There is no laboratory finding specific to Behçet's disease. The levels of various routine parameters such as erythrocyte sedimentation rate and C-reactive protein have been shown to be high in Behçet's disease, however, the change in these parameters is not always consistent with clinical activity. The aim of this study was to investigate the relationship between Behçet's disease and ischemia-modified albumin, methylglyoxal and serum prolidase activity.
Materials and Methods: 35 Behçet patients and 35 controls were included in to the study. Serum ischemia-modified albumin levels and prolidase activity were measured spectrophotometrically (Perkin Elmer Lambda 25 UV/Vis, US) and serum methylglyoxal levels were measured by Thermo Ultimate 3000 Ultra-High Performance Liquid Chromatography.
Results: In the Behcet group, serum ischemia-modified albumin (p <0.001), prolidase, methylglyoxal (p <0.001), neutrophil lymphocyte ratio (p = 0.011), platelet lymphocyte ratio (0.015), erythrocyte sedimentation rate (p = 0.047), erythrocyte distribution width (p=0.021), neutrophil (p=0.007) levels were found to be significantly higher than the control group, whereas mean corpuscular hemoglobin concentration value was found to be significantly lower than the control group (p = 0010).
Conclusions: Serum ischemia modified albumin, methylglioxal levels and prolidase activity were statistically significantly different in the Behçet group compared to the control group.

Behçet Hastalarında Metilglioksal, İskemi Modifiye Albumin Düzeyleri ve Prolidaz Aktivitesinin Araştırılması

Abstract

Amaç: Behçet hastalığı otoimmün, kronik, enflamatuvar, multisistemik bir hastalıktır. Hastalıkta yaygın olarak oral aft, genital ülserler, cilt lezyonları, üveit görülmekle birlikte temel patolojik bulgu vaskülittir. Behçet hastalığının spesifik bir laboratuvar bulgusu yoktur. Behçet hastalığında eritrosit sedimentasyon hızı, C-reaktif protein gibi çeşitli rutin parametrelerin düzeylerinde yükseklikler belirtilmekle birlikte bu parametrelerin düzeylerindeki değişiklikler klinik aktiviteyle her zaman paralellik göstermemektedir. Bizim bu çalışmadaki amacımız Behçet hastalığı ile iskemi modifiye albumin, metilglioksal ve serum prolidaz aktivitesi arasındaki ilişkiyi ortaya koymaktır.
Materyal ve metod: Çalışmaya 35 Behçet hastası, 35 kontrol dahil edilmiştir. Tüm bireylerin metilglioksal düzeyleri kromatografik yöntemle Thermo Ultimate 3000 Ultra-Yüksek Performanslı Likit Kromatografisi cihazında, serum iskemi modifiye albumin düzeyleri ve prolidaz aktivitesi spektrofotometrik (Perkin Elmer Lambda 25 UV/Vis, US ) yöntemle ölçülmüştür.
Bulgular: Behçet grubunda serum iskemi modifiye albumin (p<0.001), prolidaz, metilglioksal (p<0.001), nötrofil lenfosit oranı (p=0.011), platelet lenfosit oranı (0.015), eritrosit sedimentasyon hızı (p=0.047), eritrosit dağılım genişliği (p=0.021), nötrofil(p=0.007) düzeyleri kontrol grubuna göre istatistiksel olarak belirgin düzeyde yüksek bulunurken, ortalama eritrosit hemoglobin konsantrasyonu değeri ise kontrol grubuna göre istatistiksel olarak belirgin düzeyde düşük bulunmuştur (p=0.010).
Sonuç: Serum iskemi modifiye albumin, metilglioksal düzeyleri ve prolidaz aktivitesi Behçet grubunda kontrol grubuna göre istatistiksel olarak belirgin düzeyde farklı bulunmuştur.

Keywords

• Behçet
• metilglioksal
• prolidaz

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