Biyofilm pozitif Staphylococcus epidermidis icaA gen ekspresyonuna, Lactobacillus acidophilus ATCC 4356 kökeninin etkisinin in-vitro incelenmesi

Year 2018, Volume: 15 Issue: 3, 186 - 189, 12.12.2018

Mehmet Demirci , Akın Yiğin

Abstract

Amaç: S.
epidermidis biyofilm
oluşumu, bu bakterilerin antibakteriyel ilaçlara ve bağışıklık sistemi
savunmasına karşı koruyan önemli bir bariyer görevi oluşturmaktadır. IcaA geni
bunun oluşumu için önemlidir. Lactobacillus türlerinin probiyotik etkileri
bilinmektedir. Bizde çalışmamızda, CRA yöntemi ile biyofilm pozitifliği
saptanan S. epidermidis kökenlerinin icaA gen ekspresyonuna, L. acidophilus ATCC 4356 kökeninin
in-vitro etkisinin incelenmesi amaçladık.

Materyal ve metod: Haziran – Aralık 2017 döneminde klinik örneklerden
izole edilmiş olan ve CRA metodu ile biyofilm üretimi pozitif olarak saptanan
yirmi (20) S. epidermidis kökeni
çalışmamıza dahil edildi. CRA’da siyah pozitif kolonilerden, 2 McFarland’lık
süspansiyonundan 250 mL
alınarak, L. acidophilus içeren ve içermeyen 2 tüpe ilave edildi ve %5CO₂’li
ortamda 37^oC’da inkübasyona bırakıldı. Inkübasyonun 6. saatinde,
tüplerden RNA izolasyonları gerçekleştirildi. cDNA sentezi sonrası, spesifik
primerler ile LightCycler480 sisteminde real-time PCR yöntemi ile çalışıldı.
Gruplara ait sonuçlar delta delta Ct yöntemi ile oluşturularak, Mann-Whitney U
testi ile istatistiksel olarak incelendi.

Bulgular: L.
acidophilus ATCC 4356
etkileşimi sonrasında S. epidermidis
kökenlerinde icaA gen ekspresyon
seviyesinde gözlenen upregulasyon, istatistiksel olarak anlamlı düzeyde bulundu
(p<0.0001).

Sonuç: Sonuç olarak, çalışmamızda, in-vitro olarak, biyofilm
yeteneği olan S. epidermidis
kökenlerinin probiyotik etkili L.
acidophilus ile etkileşimi sonrası icaA
gen ekspresyon düzeyinde upregulasyon gösterdiğini saptadık. Diğer
probiyotikleri de kapsayacak şekilde daha geniş kapsamlı bakteri-bakteri
etkileşimi çalışmalarının yapılarak yeni stratejilerin geliştirilebileceği
kanaatindeyiz.

Keywords

Lactobacillus acidophilus, biyofilm, Staphyloccoccus epidermidis, icaA gen ekspresyonu

Investigation of in-vitro effects of L. acidophilus ATCC 4356 strain on icaA gene expression of biofilm positive Staphylococcus epidermidis

Abstract

Background: The formation of S.
epidermidis biofilm is an important barrier to protecting these bacteria
against antibacterial drugs and defense of the immune system. IcaA gene is important for the formation
of this. The probiotic effects of Lactobacillus
species are known. Aim of this study was to investigate the in-vitro effect of L. acidophilus ATCC 4356 genotype on the
expression of the icaA gene in S. epidermidis strains, which were found to be
positively biofilm with CRA method.

Methods: Between June and December 2017, Twenty (20) S. epidermidis strains isolated from
clinical specimens and detected as positive for biofilm production by CRA
method were included the study. 250 μL of 2 McFarland suspension of
black-positive colonies from the CRA were added to 2 vials containing with and without
L. acidophilus and incubation was
carried out at 37°C in 5% CO2. At the 6^th hour of incubation, RNA
was isolated from these tubes. After cDNA synthesis, real-time PCR was
performed on the LightCycler480 system with specific primers. The results of
groups by delta delta Ct method were statistically analyzed by Mann-Whitney U
test.

Results: The upregulation at the level of icaA gene expression in S.
epidermidis strains after L.
acidophilus ATCC 4356 interaction was found statistically significant (p
<0.0001) according to without L.
acidophilus.

Conclusions: In conclusion, our study showed that in-vitro biofilm
strains of S. epidermidis showed
upregulation of icaA gene expression
ratio after interaction with probiotic L.
acidophilus. We believe that new strategies can be developed by conducting
broader, bacterial-bacterial interaction studies, including other probiotics.

Keywords

Lactobacillus acidophilus, biofilm, Staphyloccoccus epidermidis, icaA gene expression

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