Bilgisayarlı Tomografinin oksidatif stres parametreleri üzerine etkisi

Year 2012, Volume: 9 Issue: 3, 99 - 103, 01.12.2012

Sema Yıldız Hasan Çeçe Zeynep Aktı İhsan Kaya Hakim Çelik Hale Çakır Abdullah Taşkın Nurten Aksoy

Abstract

Amaç: Bu çalışmanın amacı, kontrastsız bilgisayarlı tomografi BT sonrası erken dönem serum oksidatif stres belirteçlerinden olan seruloplazmin, lipit hidroperoksit LOOH ve total serbest sülfidril gruplarındaki -SH değişikliklerin saptanmasıdır.Materyal ve metod: Çalışmaya spiral BT cihazı Toshiba X-vision ,Tokyo, Japonya ile kontrastsız kraniyal tomografi çekilen 28 hasta dahil edildi. Venöz kan örneklemleri tetkik öncesi grup I ve kontrastsız BT tetkikinden hemen sonra grup II elde edildi. Serum seruloplazmin seviyeleri otomatik kolorimetrik metotla, serum LOOH seviyeleri ferröz iyon oksidasyonu 'xylenol orange' FOX-2 yöntemiyle çalışıldı. Sülfidril gruplarının konsantrasyonu, serbest sülfidril grubu olarak redükte glutatyonun spektrofotometrik olarak hesaplanmasıyla elde edildi. Her olguya ait iki grubu içeren serum oksidatif stress parametrelerinin kıyaslanmasında bağımlı örneklem t- testi kullanıldı.Bulgular: Serum LOOH 4,71±1,43'e karşı 5,13±1,53 μmol tBLOOH/L, p=0,179 ve –SH seviyeleri 0,311±,029'e karşı 0,305±0,025 mmol/L, p=0,084 yönünden grup I ve grup II arasında anlamlı fark saptanmadı. Grup II'nin serum seruloplazmin seviyesi grup I'e göre anlamlı olarak düşük saptanmıştır. 741,7±70,7'e karşı 757,4±63,6 U/L, p=0,015 . Sonuç: BT'nin bilinen diğer etkilerinin yanında, bu çalışma ile kontrastsız BT sonrası erken dönemde serum LOOH ve –SH düzeyleri değişmese de serum seruloplazmin düzeylerinde belirgin azalma saptanmıştır ve bu konuda daha uzun takip sürelerini içeren ileri çalışmalara ihtiyaç vardır

Keywords

Bilgisayarlı tomografi, oksidatif stres, lipit hidroperoksit, seruloplazmin, total serbest sülfidril grupları

Impact of Computed Tomography on oxidative stress

Abstract

Background: We aimed to assess the impact of computed tomography CT on serum oxidative stress markers of ceruloplasmin, lipid hydroperoxide LOOH and total free sulfhydryl groups –SH in patients undergoing non contrastenhanced CT.Methods: The study included 28 patients undergoing non contrast-enhanced cranial CT with spiral CT Toshiba Xvision , Tokyo, Japan . Venous blood samples were withdrawn at baseline Group I , and after non contrast-enhanced CT Group II . Serum ceruloplasmin levels were determined with an automatic colorimetric method, whereas serum LOOH levels were determined by the ferrous ion oxidation-xylenol orange FOX-2 method based on a known principle of the oxidation of Fe II to Fe III by lipid hydroperoxides, under acidic conditions. The concentration of sulfhydryl groups was calculated spectrophotometrically using reduced glutathione as the free sulfhydryl group. Comparison of serum oxidative stress markers among two groups of samples from each case were performed with paired samples t-test.Results: Serum LOOH 4.71±1.43 vs. 5.13±1.53 μmol tBLOOH/L, respectively, p=0.179 and –SH levels .311±.029 and .305±.025 mmol/L respectively, p=0.084 were not significantly different between Groups I and II. Serum ceruloplasmin was significantly decreased in Group II compared to Group I 741.7±70.7 vs.757.4±63.6 U/L respectively, p=0.015 . Conclusions: Besides the previous studies revealing deleterious effects of CT, the present study has shown decreased serum ceruloplasmin levels [besides unchanged serum LOOH and –SH levels] after performing non contrast-enhanced CT compared to baseline that might reveal a novel untoward effect of CT which should be reinforced with further researches

Keywords

Ceruloplasmin, computed tomography, lipid hydroperoxide, oxidative stress, total free sulfhydryl groups

References

• ) Neal R, Matthews RH, Lutz P, Ercal N. Antioxidant role of N-acetyl cysteine isomers following high dose irradiation. Free Radic Biol Med 2003; 34: 689–95.
• ) Weiss JF. Pharmacologic approaches to protection against radiation-induced lethality and other damage. Environ Health Perspect 1997; 105: 1473–8.
• ) Cochrane CG. Cellular injury by oxidants. Am J Med ; 91: 23S–30S. ) Swierczynski J, Kochan Z, Mayer D. Dietary -tocopherol prevents dehydropiandrosterone-induced lipid peroxidation in rat liver microsomes and mitochondria. Toxicol Lett 1997; : 129–36.
• ) Sies H. Oxidative stress: from basic research to clinical application. Am J Med 1991; 91: 31S–8S.
• ) Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress- induced cancer. Chem Biol Interact 2006; 160: 1–40.
• ) Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39: 44–84.
• ) Steel GG. Basic clinical radiobiology. 3rd ed. New York: Edward Arnold Ltd. 2002; 2–4.
• ) Healy J, Tipton K. Ceruloplasmin and what it might do. J Neural Transm 2007; 114: 777–81.
• ) Memişoğulları R, Bakan E. Levels of ceruloplasmin, transferrin, and lipid peroxidation in the serum of patients with Type 2 diabetes mellitus. Journal of Diabetes and Its Complications 2004; 18: 193-7.
• ) Daimon M, Hama K, Susa S, Kimura M, Yamatani K, Ohnuma H, Manaka H, Kato T. Hyperglycemia is a factor for an increase in serum ceruloplasmin in type 2 diabetes. Diabetes Care 1998; 21: 1525–8.
• ) Ryrfeldt A, Bannenberg G, Moldeus P. Free radicals and lung disease. British Med Bull 1993; 49: 588–603.
• ) Brix G, Lechel U, Veit R, Truckenbrodt R, Stamm G, Coppenrath EM, Griebel J, Nagel HD. Assessment of a theoretical formalism for dose estimation in CT: an anthropomorphic phantom study. Eur Radiol 2004; 14: –84.
• ) Erel O. Automated measurement of serum ferroxidase activity. Clin Chem 1998; 44: 2313-9.
• ) Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Biochem 2004; 37: 112–9.
• ) Arab K, Steghens JP. Plasma lipid hydroperoxides measurement by an automated xylenol orange method. Anal Biochem 2004; 325: 158–63.
• ) Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82: 70–7.
• ) Hu ML, Louie S, Cross CE, Motchnik P, Halliwell B. Antioxidant protection against hyochlorous acid in human plasma. J Lab Clin Med 1993; 121: 257–62.
• ) Halliwell B, Gutteridge JMC. An introduction to oxygen toxicity and free radicals. In: Free Radic Biol Med. Clarendon Press, Oxford 1999: 18–24.
• )Gajdusek CM, Tian H, London S, Zhou D, Rasey J, Mayberg MR. Gamma radiation effect vascular smooth muscle cells in culture. Int J Radiat Oncol Biol Phys 1996; 36: –8.
• )Bonnefont-Rousselot D, Gardès-Albert M, Delattre J, Ferradini C. Oxidation of low-density lipoproteins by OH and OH/O-2 free radicals produced by gamma radiolysis. Radiat Res 1993; 134: 271–82.
• ) Cooke MS, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNA damage: mechanisms, mutation, and disease. Faseb J ; 17: 1195–214.
• ) Barker S, Weinfeld M, Zheng J, Li L, Murray D. Identification of mammalian proteins cross-linked to DNA by ionizing radiation. J Biol Chem 2005; 280: 3326–38.
• ) De Flora S, Izzotti A, D'Agostini F, Balansky RM. Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking- related end-points. Carcinogenesis 2001; 22: 999–1013.
• ) Hofer M, Mazur L, Pospísil M, Weiterová L, Znojil V. Radioprotective action of extracellular adenosine on bone marrow cells in mice exposed to gamma rays as assayed by the micronucleus test. Radiat Res 2000; 154: 217–21.
• ) Lloyd DC, Edwards AA, Prosser JS, Corp MJ. The dose response relationship obtained at constant irradiation times for the induction of chromosome aberrations in human lymphocytes by cobalt-60 gamma rays. Radiat Environ Biophys 1984; 23: 179–89.
• ) Edwards AA, Lloyd DC, Purrott RJ. Dicentric chromosome aberration yield in human lymphocytes and radiation quality; A resume including recent results using alpha-particles. In: Booz J, Ebert HG, Hartfiel HD, editors. Seventh Symposium on Microdosimetry, Report EUR7147. Chur: Harwood Academic Publishers 1980: 1263–73.
• ) Tawn EJ, Whitehouse CA, Martin FA. Sequential chromosome aberration analysis following radiotherapy - no evidence for enhanced genomic instability. Mutat Res 2000; : 45–51.
• ) Sinha M, Das DK, Bhattacharjee S, Majumdar S, Dey S. Leaf extract of Moringa oleifera prevents ionizing radiation- induced oxidative stress in mice. J Med Food. 2011; 14: –72.
• ) Yoshida T, Goto S, Kawakatsu M, Urata Y, Li TS. Mitochondrial dysfunction, a probable cause of persistent oxidative stress after exposure to ionizing radiation. Free Radic Res 2012; 46: 147–53.
• ) Simone NL, Soule BP, Ly D, Saleh AD, Savage JE, Degraff W, et al. Ionizing radiation-induced oxidative stress alters miRNA expression. Plos One 2009; 27; 4: e6377.
• ) Winyard PG, Hider RC, Brailsford S, Drake AF, Lunec J, Blake DR. Effects of oxidative stress on some physiochemical properties of caeruloplasmin. Biochem J 1989; 258: 435–45.