Resveratrol Karbon Noktalarının SH-SY5Y İnsan Nöroblastoma Hücrelerinde 6-OHDA Kaynaklı Nöronal Hücre Ölümü Üzerinde Koruyucu Etkileri

Year 2023, Volume: 20 Issue: 3, 508 - 512, 31.12.2023

Betul Cicek , Betül Danışman

https://doi.org/10.35440/hutfd.1318802

Abstract

Amaç: Bu çalışmada Resveratrol karbon noktalarının (RES-KN) 6-OHDA'nın SH-SY5Y insan nöroblastoma hücrelerininde neden olduğu oksidoinflamatuar stres ve apoptozdan koruma potansiyelini araştırmayı amaçladık.
Materyal ve Metod: SH-SY5Y hücreleri, in vitro PH modelini indüklemek için 24 saat boyunca 200 μM 6-OHDA'ya maruz bırakıldı. Hücrelere, 6-OHDA uygulamasından 30 dakika önce farklı konsantrasyonlarda RES KN (12.5, 25, and 50 µg/mL) uygulandı.
Bulgular: Özellikle, RES KN uygulaması sonucu 6-OHDA'nın neden olduğu hücre ölümü etkili bir şekilde engellendiğini ve SH-SY5Y hücrelerinde hücre canlılığı önemli ölçüde korunduğunu gözlemledik. RES-KN, SH-SY5Y hücrelerinde toplam antioksidanları güçlendirerek ve toplam oksidanları düşürerek 6-OHDA kaynaklı oksidatif hasarı önledi. Benzer şekilde, RES-KN, 6-OHDA kaynaklı inflamatuvar faktörlerin (TNF-a ve IL-1β) salınımını önemli ölçüde azalttı. Ayrıca RES-KN, kaspaz-3 mRNA ekspresyonunu baskılayarak 6-OHDA’nın neden olduğu apoptozu engelledi.
Sonuç: RES-KN’lar, oksido-inflamatuar ve apoptotik yanıtı modüle ederek SH-SY5Y hücrelerini 6-OHDA kaynaklı nörotoksisiteden kurtardı. Bu çalışma, RES’den sentezlenen KN’lerin PH tedavisinde umut verici terapötik potansiyele sahip olabileceğini düşündürmektedir.

Keywords

Parkinson's disease, 6-OHDA, RES C Dots, SH-SY5Y cells

Protective Effects of Resveratrol Carbon Dots Against 6-OHDA-Induced Neurotoxicity in SH-SY5Y Cells

Abstract

Background: We aimed to investigate the ability of resveratrol carbon dots (RES C-Dots) to protect SH-SY5Y cells from oxido-inflammatory stress and apoptosis caused by 6-hydroxydopamine (6-OHDA).
Materials and Methods: In vitro PD model was generated in SH-SY5Y cells by administering of 200 µM 6-OHDA for 24 hours. Different concentrations of RES C-Dots (12.5, 25, and 50 µg/mL) were applied to the cells 30 minutes before administration of 6-OHDA.
Results: We observed that application of RES C-Dots prevented cell death induced by 6-OHDA and main-tained cell viability. As expected, RES C-Dots prevented oxidative damage induced by 6-OHDA - by strengthening the total amount of antioxidants and lowering the total amount of oxidants in SH-SY5Y cells. Similarly, RES C-Dots markedly alleviated the secretion of inflammatory factors (TNF-α and IL-1β) promot-ed by 6-OHDA. Furthermore, RES C-Dots prevented apoptosis induced by 6-OHDA by suppressing caspase-3 mRNA expression level.
Conclusions: RES C-Dots rescued SH-SY5Y cells from 6-OHDA- induced damage by modulating the oxido-inflammatory and apoptotic response. This report indicates enounces that RES- synthesised C-Dots may have promising curative potential for PD.

Keywords

Parkinson Disease, 6-OHDA, RES C-Dots, SH-SY5Y cells

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