Research Article
BibTex RIS Cite

Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters

Year 2023, Volume: 12 Issue: 2, 134 - 140, 22.12.2023
https://doi.org/10.31196/huvfd.1301399

Abstract

This study aims to determine the morphological differences according to gender via radiographic images taken from the carpal bones of English setters. A total of 28 bone samples, 16 females and 12 males, were used in the study. The shape differences between males and females were evaluated by placing 27 landmarks on the X-ray images. Principal Component Analyses are used to measure the distribution of variance. In addition, these analyses take the mean shape variation and compare it to shape differences. As a result of this analysis, the value of PC1 explained 24.34% of the total variation. In addition, PC2 explained 16.24% of the total variation, and PC3 explained 10.45%. In addition, the results of PCA were also described in terms of the English setter samples used. Then, Discriminant Function analysis was performed using the same software program. With this analysis, it was tried to determine the differences between the genders. According to the discriminant function test, 13 of 16 female dogs were completely separated from male dogs. Also, all 12 male dogs were separated from female dogs according to the same test results. In short, because of this study, shape differences in the carpal bones of English setters were investigated according to gender.

References

  • Barnes AE, Case DT, Burnett SE, Mahakkanukrauh P, 2020: Sex estimation from the carpal bones in a Thai population. Aust J Forensic Sci, 52 (6), 665-680.
  • Bidmos MA, Steinberg N, Kuykendall KL, 2005: Patella measurements of South African whites as sex assessors. Homo, 56 (1), 69-74.
  • Bookstein FL, Grayson B, Cutting CB, Kim HC, McCarthy JG, 1991: Landmarks in three dimensions: reconstruction from cephalograms versus direct observation. Am J Orthod Dentofacial Orthop, 100 (2), 133-140.
  • Demiraslan Y, Dayan MO, 2021: Veteriner Sistemik Anatomi. 1st ed., Nobel Tıp Kitabevleri, Ankara, Türkiye.
  • Demircioglu I, Duro S, Gungoren G, Choudhary OP, Gundemir O, Demiraslan Y, Pazvant G, 2022: Digits angle and digits length ratio in japanese quail (Coturnix coturnix japonica). Indian J Anim Res, 56 (9), 1105-1109.
  • Duro S, Gundemir O, Sönmez B, Jashari T, Szara T, Pazvant G, Kambo A, 2021: A different perspective on sex dimorphism in the adult Hermann's tortoise: geometric morphometry. Zool Stud, 60: e9.
  • Dursun N, 2007: Veteriner Anatomi 1, Medisan Yayınevi, Ankara, Türkiye. Evans HE, De Lahunta A, 2012: Miller's anatomy of the dog-E-Book. 4th ed., Elsevier health sciences, Missouri, MO, USA.
  • Giurazza F, Schena E, Del Vescovo R, Cazzato RL, Mortato L, Saccomandi P, Zobel BB, 2013: Sex determination from scapular length measurements by CT scans images in a Caucasian population. In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, (EMBC) (pp. 1632-1635).
  • González-Rellán S, Barreiro A, Cifuentes JM, Fdz-de-Trocóniz P, 2022: Anatomy of the PaLar Region of the Carpus of the Dog. Animals, 12 (12), 1573.
  • Gundemir MG, Szara T, Spataru C, Demircioglu I, Turek B, Petrovas G, Spataru MC, 2023a: Shape differences of the Carina sterni in birds of various locomotion types. Anat Histol Embryol, 52 (2), 190-196.
  • Gundemir O, 2022: Skull of the Asian (Paradoxurus Hermaphroditus) and the golden (Paradoxurus Zeylonensis) paL civet: Geometric morphometric analysis using palate, tooth and frontal landmarks. Anat Histol Embryol, 51 (6), 718-727.
  • Gundemir O, Duro S, Jashari T, Kahvecioglu O, Demircioglu İ, Mehmeti H, 2020a: A study on morphology and morphometric parameters on skull of the Bardhoka autochthonous sheep breed in Kosovo. Anat Histol Embryol, 49 (3), 365-371.
  • Gundemir O, Hadžiomerović N, Pazvant G, Erdikmen DO, 2021: Radiometric and geometric morphometric analysis of the carpal joint area in 2-year-old Thoroughbred horses. Veterinaria, 70 (2), 209-217.
  • Gundemir O, Özkan E, Dayan MO, Aydogdu S, 2020b: Sexual analysis in turkey (Meleagris gallopavo) neurocranium usinggeometricmorphometric methods. Turk J Vet Anim Sci, 44 (3), 681-687.
  • Gundemir O, Szara T, Yalin EE, Karabagli M, Mutlu Z, Yilmaz O, Parés-Casanova PM, 2023b: Examination of Shape Variation of the Skull in British Shorthair, Scottish Fold, and Van Cats. Animals, 13 (4), 614.
  • Gurbuz İ, Demiraslan Y, Rajapakse C, Weerakoon DK, Fernando S, Spataru MC, Gundemir O, 2022: Skull of the Asian (Paradoxurus Hermaphroditus) and the golden (Paradoxurus Zeylonensis) palm civet: Geometric morphometric analysis using palate, tooth and frontal landmarks Anat Histol Embryol, 51 (6), 718-727.
  • Horst Erich König, Hans-Georg Liebich, 2007: Veterinary Anatomy of Domestic Mammals. 3rd ed., Schattauer, Stuttgart, Germany.
  • International Committee on Veterinary Gross Anatomical Nomenclature (I.C.V.G.A.N.), 2017: Nomina Anatomica Veterinaria, 6th ed., Published by the Editorial Committee, Hanover, Germany, Ghent, Belgium, Columbia, MO, USA, Rio de Janeiro, Brazil.
  • Introna Jr F, Di Vella G, Campobasso CP, 1998: Sex determination by discriminant analysis of patella measurements. Forensic Sci Int, 95 (1), 39-45.
  • Irion DN, Schaffer AL, Famula TR, Eggleston ML, Hughes SS, Pedersen NC, 2003: Analysis of genetic variation in 28 dog breed populations with 100 microsatellite markers. J Hered, 94 (1), 81-87.
  • Jashari T, Kahvecioglu O, Duro S, Gundemir O, 2022: Morphometric analysis for the sex determination of the skull of the Deltari Ilir dog (Canis lupus familiaris) of Kosovo. Anat Histol Embryol, 51 (4), 443-451.
  • Klingenberg CP: MorphoJ, 2011: an integrated software package for geometric morphometrics. Mol Ecol Resour, 11 (2), 353-357.
  • Li A, Bennett D, Gibbs C, Carmichael S, Gibson N, Owen M, Denny HR, 2000: Radial carpal bone fractures in 15 dogs. J Small Anim Pract, 41 (2), 74-79.
  • Mastrangelo P, De Luca S, Alemán I, Botella MC, 2011b: Sex assessment from the carpals bones: discriminant function analysis in a 20th century Spanish sample. Forensic Sci Int, 206 (1-3), 216-e1.
  • Mastrangelo P, De Luca S, Sanchez-Mejorada G, 2011a: Sex assessment from carpals bones: discriminant function analysis in a contemporary Mexican sample. Forensic Sci Int, 209 (1-3), 196-e1.
  • Myo, N. Z, Po, SP, Soe, HY, Myint, HH, Khaing, KT, 2016: Comparison on Development of Ossification Centres of Radius and Ulna in Male and Female Dogs by Radiographically. IJNRLS, 3 (5), 18-29.
  • Olusa TA, Akbar Z, Murray CM, Davies HM, 2021: Morphometric analysis of the intercarpal ligaments of the equine proximal carpal bones during simulated flexion and extension of cadaver limbs. Anat Histol Embryol, 50 (1), 151-160.
  • Pedersen N, Liu H, Theilen G, Sacks B, 2013: The effects of dog breed development on genetic diversity and the relative influences of performance and conformation breeding. J Anim Breed Genet, 130 (3), 236-248.
  • Rainbow MJ, Crisco JJ, Moore DC, Wolfe SW, 2008: Gender differences in capitate kinematics are eliminated after accounting for variation in carpal size. J Biomech Eng-t Asme, 130 (4), 041003.
  • Riggs BL, Melton III LJ, Robb RA, Camp JJ, Atkinson EJ, Peterson JM, Rouleau PA, McCollough CH, Bouxsein ML, Khosla S, 2004: Population‐based study of age and sex differences in bone volumetric density, size, geometry, and structure at different skeletal sites. J Bone Miner Res, 19 (12), 1945-1954.
  • Sarač-Hadžihalilović A, Ajanović Z, Hasanbegović I, Šljuka S, Rakanović-Todić M, Aganović I, Hadžiselimović R, 2022: Analysis of gender differences on pyriform aperture of human skulls using geometric morphometric method. Folia Morphol, 81 (3), 707-714.
  • Senol E, Gundemir O, Duro S, Szara T, Demiraslan Y, Karadag H, 2022.: A pilot study: Can calcaneus radiographic image be used to determine sex and breed in cats? Vet Med Sci, 8 (5), 1855-1861.
  • Szara T, Duro S, Gundemir O, Demircioglu İ, 2022: Sex determination in Japanese Quails (Coturnix japonica) using geometric morphometrics of the skull. Animals, 12(3), 302.
  • Vatne L, Dickson D, Tidholm A, Caivano D, Rishniw M, 2021: The effects of activity, body weight, sex and age on echocardiographic values in English setter dogs. J Vet Cardiol, 37, 26-41.

İngiliz Setter Köpeklerinin Karpal Eklem Bölgesinin Radyografik Görüntüleri Üzerinde Geometrik Morfometrik Analizler

Year 2023, Volume: 12 Issue: 2, 134 - 140, 22.12.2023
https://doi.org/10.31196/huvfd.1301399

Abstract

Bu çalışmada, İngiliz setter köpeklerinin karpal kemiklerinden alınan radyografik görüntüler aracılığıyla cinsiyete göre morfolojik farklılıkların belirlenmesi amaçlanmıştır. Çalışmada 16 dişi ve 12 erkek olmak üzere toplam 28 kemik örneği kullanılmıştır. Erkek ve dişiler arasındaki şekil farklılıkları, röntgen görüntüleri üzerinde toplam 27 adet işaretleme ile değerlendirilmiştir. Varyans dağılımını ölçmek için Principal Component Analysis (PCA) kullanılmıştır. Bu analizlerin kullanım amacı ortalama şekil varyasyonunu alıp şekil farklılıklarıyla karşılaştırmaktadır. Bu analiz sonucunda PC1 değeri toplam varyasyonun %24,34'ünü açıklamıştır. Ayrıca PC2 toplam varyasyonun %16,24'ünü, PC3 ise toplam varyasyonun %10,45'ini açıklamıştır. Daha sonra örnekler Diskriminant Fonksiyon analizi üzerinden de test edilmiştir. Bu analiz ile cinsiyetler arasındaki farklılıklar belirlenmeye çalışılmıştır. Diskriminant fonksiyon testine göre 16 dişi köpeğin 13'ü erkek köpeklerden tamamen ayrılmıştır. Yine aynı testin sonuçlarına göre 12 erkek köpeğin tamamı dişi köpeklerden ayrılmıştır. Kısacası, bu çalışma sonucunda İngiliz setter köpeklerinin karpal kemiklerindeki şekil farklılıkları ve cinsiyete göre karpal kemiklerde oluşan değişimler araştırılmıştır.

References

  • Barnes AE, Case DT, Burnett SE, Mahakkanukrauh P, 2020: Sex estimation from the carpal bones in a Thai population. Aust J Forensic Sci, 52 (6), 665-680.
  • Bidmos MA, Steinberg N, Kuykendall KL, 2005: Patella measurements of South African whites as sex assessors. Homo, 56 (1), 69-74.
  • Bookstein FL, Grayson B, Cutting CB, Kim HC, McCarthy JG, 1991: Landmarks in three dimensions: reconstruction from cephalograms versus direct observation. Am J Orthod Dentofacial Orthop, 100 (2), 133-140.
  • Demiraslan Y, Dayan MO, 2021: Veteriner Sistemik Anatomi. 1st ed., Nobel Tıp Kitabevleri, Ankara, Türkiye.
  • Demircioglu I, Duro S, Gungoren G, Choudhary OP, Gundemir O, Demiraslan Y, Pazvant G, 2022: Digits angle and digits length ratio in japanese quail (Coturnix coturnix japonica). Indian J Anim Res, 56 (9), 1105-1109.
  • Duro S, Gundemir O, Sönmez B, Jashari T, Szara T, Pazvant G, Kambo A, 2021: A different perspective on sex dimorphism in the adult Hermann's tortoise: geometric morphometry. Zool Stud, 60: e9.
  • Dursun N, 2007: Veteriner Anatomi 1, Medisan Yayınevi, Ankara, Türkiye. Evans HE, De Lahunta A, 2012: Miller's anatomy of the dog-E-Book. 4th ed., Elsevier health sciences, Missouri, MO, USA.
  • Giurazza F, Schena E, Del Vescovo R, Cazzato RL, Mortato L, Saccomandi P, Zobel BB, 2013: Sex determination from scapular length measurements by CT scans images in a Caucasian population. In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, (EMBC) (pp. 1632-1635).
  • González-Rellán S, Barreiro A, Cifuentes JM, Fdz-de-Trocóniz P, 2022: Anatomy of the PaLar Region of the Carpus of the Dog. Animals, 12 (12), 1573.
  • Gundemir MG, Szara T, Spataru C, Demircioglu I, Turek B, Petrovas G, Spataru MC, 2023a: Shape differences of the Carina sterni in birds of various locomotion types. Anat Histol Embryol, 52 (2), 190-196.
  • Gundemir O, 2022: Skull of the Asian (Paradoxurus Hermaphroditus) and the golden (Paradoxurus Zeylonensis) paL civet: Geometric morphometric analysis using palate, tooth and frontal landmarks. Anat Histol Embryol, 51 (6), 718-727.
  • Gundemir O, Duro S, Jashari T, Kahvecioglu O, Demircioglu İ, Mehmeti H, 2020a: A study on morphology and morphometric parameters on skull of the Bardhoka autochthonous sheep breed in Kosovo. Anat Histol Embryol, 49 (3), 365-371.
  • Gundemir O, Hadžiomerović N, Pazvant G, Erdikmen DO, 2021: Radiometric and geometric morphometric analysis of the carpal joint area in 2-year-old Thoroughbred horses. Veterinaria, 70 (2), 209-217.
  • Gundemir O, Özkan E, Dayan MO, Aydogdu S, 2020b: Sexual analysis in turkey (Meleagris gallopavo) neurocranium usinggeometricmorphometric methods. Turk J Vet Anim Sci, 44 (3), 681-687.
  • Gundemir O, Szara T, Yalin EE, Karabagli M, Mutlu Z, Yilmaz O, Parés-Casanova PM, 2023b: Examination of Shape Variation of the Skull in British Shorthair, Scottish Fold, and Van Cats. Animals, 13 (4), 614.
  • Gurbuz İ, Demiraslan Y, Rajapakse C, Weerakoon DK, Fernando S, Spataru MC, Gundemir O, 2022: Skull of the Asian (Paradoxurus Hermaphroditus) and the golden (Paradoxurus Zeylonensis) palm civet: Geometric morphometric analysis using palate, tooth and frontal landmarks Anat Histol Embryol, 51 (6), 718-727.
  • Horst Erich König, Hans-Georg Liebich, 2007: Veterinary Anatomy of Domestic Mammals. 3rd ed., Schattauer, Stuttgart, Germany.
  • International Committee on Veterinary Gross Anatomical Nomenclature (I.C.V.G.A.N.), 2017: Nomina Anatomica Veterinaria, 6th ed., Published by the Editorial Committee, Hanover, Germany, Ghent, Belgium, Columbia, MO, USA, Rio de Janeiro, Brazil.
  • Introna Jr F, Di Vella G, Campobasso CP, 1998: Sex determination by discriminant analysis of patella measurements. Forensic Sci Int, 95 (1), 39-45.
  • Irion DN, Schaffer AL, Famula TR, Eggleston ML, Hughes SS, Pedersen NC, 2003: Analysis of genetic variation in 28 dog breed populations with 100 microsatellite markers. J Hered, 94 (1), 81-87.
  • Jashari T, Kahvecioglu O, Duro S, Gundemir O, 2022: Morphometric analysis for the sex determination of the skull of the Deltari Ilir dog (Canis lupus familiaris) of Kosovo. Anat Histol Embryol, 51 (4), 443-451.
  • Klingenberg CP: MorphoJ, 2011: an integrated software package for geometric morphometrics. Mol Ecol Resour, 11 (2), 353-357.
  • Li A, Bennett D, Gibbs C, Carmichael S, Gibson N, Owen M, Denny HR, 2000: Radial carpal bone fractures in 15 dogs. J Small Anim Pract, 41 (2), 74-79.
  • Mastrangelo P, De Luca S, Alemán I, Botella MC, 2011b: Sex assessment from the carpals bones: discriminant function analysis in a 20th century Spanish sample. Forensic Sci Int, 206 (1-3), 216-e1.
  • Mastrangelo P, De Luca S, Sanchez-Mejorada G, 2011a: Sex assessment from carpals bones: discriminant function analysis in a contemporary Mexican sample. Forensic Sci Int, 209 (1-3), 196-e1.
  • Myo, N. Z, Po, SP, Soe, HY, Myint, HH, Khaing, KT, 2016: Comparison on Development of Ossification Centres of Radius and Ulna in Male and Female Dogs by Radiographically. IJNRLS, 3 (5), 18-29.
  • Olusa TA, Akbar Z, Murray CM, Davies HM, 2021: Morphometric analysis of the intercarpal ligaments of the equine proximal carpal bones during simulated flexion and extension of cadaver limbs. Anat Histol Embryol, 50 (1), 151-160.
  • Pedersen N, Liu H, Theilen G, Sacks B, 2013: The effects of dog breed development on genetic diversity and the relative influences of performance and conformation breeding. J Anim Breed Genet, 130 (3), 236-248.
  • Rainbow MJ, Crisco JJ, Moore DC, Wolfe SW, 2008: Gender differences in capitate kinematics are eliminated after accounting for variation in carpal size. J Biomech Eng-t Asme, 130 (4), 041003.
  • Riggs BL, Melton III LJ, Robb RA, Camp JJ, Atkinson EJ, Peterson JM, Rouleau PA, McCollough CH, Bouxsein ML, Khosla S, 2004: Population‐based study of age and sex differences in bone volumetric density, size, geometry, and structure at different skeletal sites. J Bone Miner Res, 19 (12), 1945-1954.
  • Sarač-Hadžihalilović A, Ajanović Z, Hasanbegović I, Šljuka S, Rakanović-Todić M, Aganović I, Hadžiselimović R, 2022: Analysis of gender differences on pyriform aperture of human skulls using geometric morphometric method. Folia Morphol, 81 (3), 707-714.
  • Senol E, Gundemir O, Duro S, Szara T, Demiraslan Y, Karadag H, 2022.: A pilot study: Can calcaneus radiographic image be used to determine sex and breed in cats? Vet Med Sci, 8 (5), 1855-1861.
  • Szara T, Duro S, Gundemir O, Demircioglu İ, 2022: Sex determination in Japanese Quails (Coturnix japonica) using geometric morphometrics of the skull. Animals, 12(3), 302.
  • Vatne L, Dickson D, Tidholm A, Caivano D, Rishniw M, 2021: The effects of activity, body weight, sex and age on echocardiographic values in English setter dogs. J Vet Cardiol, 37, 26-41.
There are 34 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research
Authors

Yusuf Altundağ This is me 0000-0001-6364-7512

Ece Oktay 0000-0002-3117-7875

Ermiş Özkan This is me 0000-0002-5000-5075

Gülsün Pazvant This is me 0000-0001-5986-3992

Early Pub Date December 4, 2023
Publication Date December 22, 2023
Submission Date May 24, 2023
Acceptance Date August 31, 2023
Published in Issue Year 2023 Volume: 12 Issue: 2

Cite

APA Altundağ, Y., Oktay, E., Özkan, E., Pazvant, G. (2023). Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters. Harran Üniversitesi Veteriner Fakültesi Dergisi, 12(2), 134-140. https://doi.org/10.31196/huvfd.1301399
AMA Altundağ Y, Oktay E, Özkan E, Pazvant G. Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters. Harran Univ Vet Fak Derg. December 2023;12(2):134-140. doi:10.31196/huvfd.1301399
Chicago Altundağ, Yusuf, Ece Oktay, Ermiş Özkan, and Gülsün Pazvant. “Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters”. Harran Üniversitesi Veteriner Fakültesi Dergisi 12, no. 2 (December 2023): 134-40. https://doi.org/10.31196/huvfd.1301399.
EndNote Altundağ Y, Oktay E, Özkan E, Pazvant G (December 1, 2023) Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters. Harran Üniversitesi Veteriner Fakültesi Dergisi 12 2 134–140.
IEEE Y. Altundağ, E. Oktay, E. Özkan, and G. Pazvant, “Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters”, Harran Univ Vet Fak Derg, vol. 12, no. 2, pp. 134–140, 2023, doi: 10.31196/huvfd.1301399.
ISNAD Altundağ, Yusuf et al. “Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters”. Harran Üniversitesi Veteriner Fakültesi Dergisi 12/2 (December 2023), 134-140. https://doi.org/10.31196/huvfd.1301399.
JAMA Altundağ Y, Oktay E, Özkan E, Pazvant G. Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters. Harran Univ Vet Fak Derg. 2023;12:134–140.
MLA Altundağ, Yusuf et al. “Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters”. Harran Üniversitesi Veteriner Fakültesi Dergisi, vol. 12, no. 2, 2023, pp. 134-40, doi:10.31196/huvfd.1301399.
Vancouver Altundağ Y, Oktay E, Özkan E, Pazvant G. Geometric Morphometric Analyses on Radiographic Images of The Carpal Joint Region of English Setters. Harran Univ Vet Fak Derg. 2023;12(2):134-40.