Year 2018, Volume 4 , Issue 1, Pages 16 - 21 2018-04-18

Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population
Türk popülasyonunda bronkopulmoner displazi insidansı ile ilişkisinin araştırılması için rs3771150 polimorfizminin genotiplenmesi ve analizi

Ayberk AKAT [1] , Seda YILMAZ SEMERCİ [2] , Osman Mutluhan UĞUREL [3] , Ayşegül ERDEMİR [4] , Merih ÇETİNKAYA [5] , Dilek TURGUT-BALIK [6]


Objectives: rs3771150 polymorphism on IL-18RAP was chosen to be investigated in terms of determining a relationship with BPD incidence seen in preterm infants born in Turkey. 

Materials and Method: Allele and genotype frequencies of this polymorphism was analyzed from genotyping results obtained by multiplex reactions and MALDI-TOF mass spectrometry method. In addition, clinical and demographic data obtained from the patients were also inquired in this study to determine any relationship with BPD incidence. 

Results: GG genotype has been determined as the wild type genotype of rs3771150 polymorphism for the Turkish population (n=49 in control group, n=46 in BPD group). When other genotypes (AG and AA) were analyzed no significant relationship was found with BPD incidence (p>0.05).

Conclusion: No significant relationship could be determined between rs3771150 polymorphism and BPD incidence in 192 preterm Turkish infants.

Amaç: Bu çalışmada Türkiye’de erken doğan bebeklerde görülen BPD insidansı ile IL-18RAP üzerinde bulunan rs3771150 polimorfizmi arasındaki ilişki araştırılmıştır. 

Materyal ve Metod: Bu polimorfizmin alel ve genotip frekansları multipleks reaksiyonlar ve MALDI-TOF kütle spektrometresi yöntemi ile elde edilen genotipleme sonuçları kullanılarak analiz edilmiştir. Buna ek olarak hastalardan elde edilen klinik ve demografik veriler de BPD insidansı ile ilişkileri açısından değerlendirilmiştir. 

Bulgular: Türk popülasyonu için rs3771150 polimorfizminin yabanıl genotipi GG olarak belirlenmiştir (n=49 kontrol, n=46 BPD hastası). Diğer genotipler (AG ve AA) analiz edildiğinde BPD insidansı ile anlamlı bir ilişki kurulamamıştır (p>0.05).

Sonuç: Erken doğan 192 Türk bebekte rs3771150 polimorfizmi ile BPD insidansı arasında anlamlı bir ilişki bulunamamıştır.

Bronchopulmonary dysplasia, multiplex polymerase chain reaction, single nucleotide polymorphism, SNP genotyping
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Primary Language en
Subjects Health Care Sciences and Services
Journal Section Articles
Authors

Author: Ayberk AKAT

Author: Seda YILMAZ SEMERCİ

Author: Osman Mutluhan UĞUREL

Author: Ayşegül ERDEMİR

Author: Merih ÇETİNKAYA

Author: Dilek TURGUT-BALIK (Primary Author)

Dates

Publication Date : April 18, 2018

Bibtex @research article { ibufntd448922, journal = {İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi}, issn = {2149-4401}, eissn = {2547-9466}, address = {}, publisher = {Bayçınar Tıbbi Yayıncılık ve Reklam Hiz. Tic. Ltd. Şti.}, year = {2018}, volume = {4}, pages = {16 - 21}, doi = {}, title = {Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population}, key = {cite}, author = {Akat, Ayberk and Yılmaz Semerci̇, Seda and Uğurel, Osman Mutluhan and Erdemi̇r, Ayşegül and Çeti̇nkaya, Merih and Turgut-balık, Dilek} }
APA Akat, A , Yılmaz Semerci̇, S , Uğurel, O , Erdemi̇r, A , Çeti̇nkaya, M , Turgut-balık, D . (2018). Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population . İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi , 4 (1) , 16-21 . Retrieved from https://dergipark.org.tr/en/pub/ibufntd/issue/38652/448922
MLA Akat, A , Yılmaz Semerci̇, S , Uğurel, O , Erdemi̇r, A , Çeti̇nkaya, M , Turgut-balık, D . "Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population" . İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi 4 (2018 ): 16-21 <https://dergipark.org.tr/en/pub/ibufntd/issue/38652/448922>
Chicago Akat, A , Yılmaz Semerci̇, S , Uğurel, O , Erdemi̇r, A , Çeti̇nkaya, M , Turgut-balık, D . "Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population". İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi 4 (2018 ): 16-21
RIS TY - JOUR T1 - Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population AU - Ayberk Akat , Seda Yılmaz Semerci̇ , Osman Mutluhan Uğurel , Ayşegül Erdemi̇r , Merih Çeti̇nkaya , Dilek Turgut-balık Y1 - 2018 PY - 2018 N1 - DO - T2 - İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi JF - Journal JO - JOR SP - 16 EP - 21 VL - 4 IS - 1 SN - 2149-4401-2547-9466 M3 - UR - Y2 - 2020 ER -
EndNote %0 İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population %A Ayberk Akat , Seda Yılmaz Semerci̇ , Osman Mutluhan Uğurel , Ayşegül Erdemi̇r , Merih Çeti̇nkaya , Dilek Turgut-balık %T Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population %D 2018 %J İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi %P 2149-4401-2547-9466 %V 4 %N 1 %R %U
ISNAD Akat, Ayberk , Yılmaz Semerci̇, Seda , Uğurel, Osman Mutluhan , Erdemi̇r, Ayşegül , Çeti̇nkaya, Merih , Turgut-balık, Dilek . "Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population". İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi 4 / 1 (April 2018): 16-21 .
AMA Akat A , Yılmaz Semerci̇ S , Uğurel O , Erdemi̇r A , Çeti̇nkaya M , Turgut-balık D . Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population. İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi. 2018; 4(1): 16-21.
Vancouver Akat A , Yılmaz Semerci̇ S , Uğurel O , Erdemi̇r A , Çeti̇nkaya M , Turgut-balık D . Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population. İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi. 2018; 4(1): 16-21.
IEEE A. Akat , S. Yılmaz Semerci̇ , O. Uğurel , A. Erdemi̇r , M. Çeti̇nkaya and D. Turgut-balık , "Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population", İstanbul Bilim Üniversitesi Florence Nightingale Tıp Dergisi, vol. 4, no. 1, pp. 16-21, Apr. 2018