Antioxidant activity, total phenolic compounds and ld50 bioassay (toxicity) activity of the fermented wood “Nikhra” fractions of combretum hartmannianum, terminalia laxiflora and acacia seyal

Year 2020, Volume: 4 Issue: 2, 298 - 302, 30.12.2020

Abdalbasit Marıod , Noha Fadl , Hiba Abdel Rahman Ali Alfatih Ahmed Hassan

Abstract

This study characterizes antioxidant activity, total phenolic compounds and LD50 bioassay (Toxicity) of the fermented wood “Nikhra” fractions of Combretum hartmannianum, Acacia seyal and Terminalia laxiflora. Antioxidant potential using DPPH assay, the content of total phenolic compounds in the fractions was determined spectrometrically according to the Folin−Ciocalteu procedure and calculated as gallic acid equivalents (GAE) and toxicity using the brine shrimp Artemia salina standard method. These trees exhibited far stronger antioxidant activity and contained significantly higher levels of phenolics. All fractions proved to be non toxic against A. salina expect ethyl acetate and chloroform fractions of A. seyal and chloroform fractions of C. hartmannianum which possessed slight toxicity.

Keywords

Antioxidant activity;, Phenolic compounds;, Phenolic acids;, Flavonoids;, Tannins;, Free radical;, Toxicity activity

References

• Abdel-Farid, I., Sheded, M., and Mohamed, E. (2014). Metabolomic profiling and antioxidant activity of some Acacia species. Saudi Journal of Biological Sciences 21, 400-408. Aberoumand, A., and Deokule, S. (2008). Comparison of phenolic compounds of some edible plants of Iran and India. Pakistan Journal of Nutrition 7, 582-585.
• Al-Fartosy, A. J. (2011). Antioxidant properties of methanolic extract of Inula graveolens L. Turkish Journal of Agriculture and Forestry 35, 591-596.
• Boersma, B. J., Patel, R. P., Kirk, M., Jackson, P. L., Muccio, D., Darley-Usmar, V. M., and Barnes, S. (1999). Chlorination and nitration of soy isoflavones. Archives of biochemistry and biophysics 368, 265-275.
• Chang, S.-T., and Tung, Y.-T. (2009). Acacia extracts and their compounds on inhibition of xanthine oxidase. Google Patents.
• Chun, S.-S., Vattem, D. A., Lin, Y.-T., and Shetty, K. (2005). Phenolic antioxidants from Clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry 40, 809-816.
• Dudonne, S., Vitrac, X., Coutiere, P., Woillez, M., and Mérillon, J.-M. (2009). Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. Journal of Agricultural and Food Chemistry 57, 1768-1774. DOI: 10.1021/jf803011r
• Estevinho, L., Pereira, A. P., Moreira, L., Dias, L. G., and Pereira, E. (2008). Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food and Chemical Toxicology 46, 3774-3779. doi.org/10.1016/j.fct.2008.09.062
• Fyhrquist, P., Mwasumbi, L., Hæggström, C.-A., Vuorela, H., Hiltunen, R., and Vuorela, P. (2002). Ethnobotanical and antimicrobial investigation on some species of Terminalia and Combretum (Combretaceae) growing in Tanzania. Journal of Ethnopharmacology 79, 169-177. DOI: 10.1016/s0378-8741(01)00375-0
• Halliwell, B., and Gutteridge, J. M. (1995). The definition and measurement of antioxidants in biological systems. Free Radical Biology and Medicine 18, 125-126.
• Hassan, L. E., Ahamed, M. B., Majid, A. S., Baharetha, H., Muslim, N. S., Nassar, Z. D., and Majid, A. M. (2014). Correlation of antiangiogenic, antioxidant and cytotoxic activities of some Sudanese medicinal plants with phenolic and flavonoid contents. BMC Complementary and Alternative Medicine 14, 406. https://doi.org/10.1186/1472-6882-14-406
• John B Hall. (1994). NFTA 94-07. School of Agriculture and Forest Sciences, University of Wales, Bangor, Gwynedd LL57 2UV, UK.
• Kamali, H., and Mohammed, M. (2007). Antibacterial activity of Hibiscus sabdariffa, Acacia seyal var. seyal and Sphaeranthus suaveolens var. suaveolens against upper respiratory tract pathogens. Sudan Journal of Medical Sciences 1, 121-126.
• Khalaf, N. A., Shakya, A. K., Al-Othman, A., El-Agbar, Z., and Farah, H. (2008). Antioxidant activity of some common plants. Turkish Journal of Biology 32: 51-55
• Luís, Â., Domingues, F., Gil, C., and Duarte, A. P. (2009). Antioxidant activity of extracts of Portuguese shrubs: Pterospartum tridentatum, Cytisus scoparius and Erica spp. Journal of Medicinal Plants Research 3, 886-893.
• Manach, C., Scalbert, A., Morand, C., Rémésy, C., and Jiménez, L. (2004). Polyphenols: food sources and bioavailability. The American Journal of Clinical Nutrition 79, 727-747. https://doi.org/10.1093/ajcn/79.5.727.
• Mariod, A.A., Matthäus, B. (2006). Antioxidant activities of extracts from Combretum hartmannianum and Guiera senegalensis on the oxidative stability of sunflower oil. Emirates Journal of Food and Agriculture 18 (2): 20-28. https://doi.org/10.9755/ejfa.v12i1.5136
• Masoko, P., Picard, J., and Eloff, J. (2007). The antifungal activity of twenty-four southern African Combretum species (Combretaceae). South African Journal of Botany 73, 173-183. https://doi.org/10.1016/j.sajb.2006.09.010
• Mbwambo, Z. H., Moshi, M. J., Masimba, P. J., Kapingu, M. C., and Nondo, R. S. (2007). Antimicrobial activity and brine shrimp toxicity of extracts of Terminalia brownii roots and stem. BMC Complementary and Alternative Medicine 7, 9. doi: 10.1186/1472-6882-7-9
• Meyer, B., Ferrigni, N., Putnam, J., Jacobsen, L., Nichols, D. j., and McLaughlin, J. (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica, 31, 4.
• Miller, A. L. (1996). Antioxidant flavonoids: structure, function and clinical usage. Alternative Medicine Review 1, 103-11.
• Nguta, J., and Mbaria, J. (2013). Brine shrimp toxicity and antimalarial activity of some plants traditionally used in treatment of malaria in Msambweni district of Kenya. Journal of Ethnopharmacology 148, 988-992. DOI: 10.1016/j.jep.2013.05.053.
• Singleton, V., Orthofer, R., and Lamuela-Raventos, R. (1999). Analysis of total phenols and other oxidation substrates. Journal of Methods in Enzymology 299, 152-178.