Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers

Abstract

The Mediterranaen cereal cyst nematode, Heterodera latipons, causing serious yield losses in protected cultivation cereal cropping areas in Turkey. An accurate and reliable identification is primarily needed to establish effective, sustainable and environmentally safe control measures to any cyst nematode species. In this study, twenty-five populations of Heterodera latipons collected from cereal areas in East-Mediterranean and South-East Anatolian regions were identified using species-specific primers (SCAR). The SCAR primer pair, H-latac F and H-latact R, defined species-specific primers were used to identify Heterodera populations. In this study stated that H-latac F and H-latact R primers for identifying of H. latipons can be efficient tools to identify the Turkish Mediterranean cereal cyst nematode populations. Moreover, the results indicated that H. latipons was the prominent cereal cyst nematode species in the East Mediterranean and South East Anatolian regions of Turkey. This clarified approach may supply a quick basis for identification of H. latipons populations for their future management and facilitate checking its distribution in the agricultural areas.

Keywords

• Heterodera latipons,identification,SCAR primer,SCAR primer

Heterodera latipons’un SCAR (Sequence Characterised Amplified Region) Primer Kullanılarak Tanımlanması

Öz

Akdeniz Tahıl kist nematodu Heterodera latipons, mücadelesinin yapılmadığı buğday üretim alanlarında önemli derece ürün kayıplarına neden olabilebilmektedir. Nematodlar ile mücadelede sürüdürülebilir ve etkili bir mücadele için nematodun türünün bilinmesi oldukça önemlidir. Bu çalışmada, Doğu Akdeniz ve Güney Doğu Anadolu bölgeleri tahıl alanlarından elde edilen 25 adet Heterodera latipons popülasyonu, türe spesifik primer çifti (SCAR) H-latacF ve H-latactR kullanılarak tanımlanmıştır. Çalışma sonucunda, Heterodera latipons popülasyonlarının tanımlanmasında H-latacF ve H-latactR primerleri etmeni başarılı bir şekilde tanımlamış olup, nematodun Doğu Akdeniz ve Güney Doğu Anadolu bölgelerindeki en önemli Tahıl kisti nematod türü olduğu ortaya konulmuştur. Ayrıca, çalışmada kullanılan türe özgü primerler Heterodera latipons popülasyonlarının kolay ve hızlı tanımlanmasında kullanılabileceği sonucuna varılmıştır. 

Anahtar Kelimeler

• Heterodera latipons,teşhis,SCAR primer

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