The Effect of Shaker Use on Embryo Yield in Shed-Microspore Cultures of Ornamental Peppers

Year 2019, Volume: 5 Issue: 2, 299 - 307, 13.12.2019

Selcen Doğan , Esin Arı

https://doi.org/10.24180/ijaws.627679

Cited By: 1

Abstract

Because of their morphological
diversity and various rich content, ornamental peppers (Capsicum annuum L.)
has become a favored plant in ornamental plant, food, cosmetics and medicinal
sectors in recent years. This interest increased the ornamental pepper breeding
studies. However, there are limited numbers of study based on haploid
technology in ornamental peppers. In this study, we aimed to determine the
effect of using shaker on embryo yield in the shed-microspore cultures of
ornamental peppers. We used a total of 29 genotypes and compared the shaken and
stationary cultures for each genotype. The shaken cultures gave more successful
androgenic performance in terms of total and globular embryo formations. The
most successful embryogenic performance was obtained from Genotype 754 in both
stationary and shaken cultures. However, the shaken cultures of Genotype 754
formed 4.5 times more embryos when compared to its stationary control group. As
the result, the use of shaker positively affected embryo yield in
shed-microspore cultures of ornamental pepper depending on genotype.

Keywords

Capsicum annuum L., doubled haploid, microspore embryogenesis, culture shaking

Supporting Institution

Scientific and Technological Research Council of Turkey

Project Number

5120019-TUBITAK-TEYDEB-1505

Çalkalayıcı Kullanımının Süs Biberi Shed-Mikrospor Kültürlerinde Embriyo Verimine Etkisi

Abstract

Morfolojik çeşitlilikleri ve
çeşitli zengin içerikleri nedeniyle süs biberleri (Capsicum annuum L.) son yıllarda süs bitkisi, gıda, kozmetik ve
ilaç sektörlerinde tercih edilen bitkiler haline gelmiştir. Süs biberine olan
bu ilgi süs biberinde yapılan ıslah çalışmalarını son yıllarda arttırmıştır.
Ancak süs biberlerinde haploid teknolojisine dayalı sınırlı sayıda ıslah
çalışması bulunmaktadır. Bu çalışmada, süs biberi shed-mikrospor kültürlerinde
çalkalayıcı kullanımının embriyo verimi üzerine etkisi belirlenmeye
çalışılmıştır. Toplam 29 süs biberi genotipi kullanılmış ve her genotipin
çalkalanan ve durağan kültürleri karşılaştırılmıştır. Çalkalanan kültürler,
toplam ve globular embriyo oluşturma açısından daha başarılı androjenik
performans göstermiştir. Çalışmada en başarılı embriyojenik performans, hem
durağan hem çalkalanan kültürlerde Genotip 754'ten elde edilmiştir. Genotip
754'ün çalkalanan kültürleri, durağan kontrol grubuna kıyasla 4.5 kat daha
fazla embriyo oluşturmuştur. Sonuç olarak, çalkalayıcı kullanımı, genotipe
bağlı olarak süs biberi shed-mikrospor kültürlerinde embriyo verimini olumlu
yönde etkilemiştir. 

Keywords

Anter, Capsicum annuum L., doubled haploid, mikrospor embriyogenesis, çalkalama

Project Number

5120019-TUBITAK-TEYDEB-1505

References

• Adelberg, J., Kroggel, M., & Toler, J. (2000). Physical environment in vitro affects laboratory and nursery growth of micropropageted hostas. Hort Technology, 10(4), 754-757.
• Adelberg, J., & Toler, J. (2004). Comparison of agar and an agitated, thin-film, liquid system for micropropagation of ornamental elephant ears. Hort Science, 39(5), 1088-1092.
• Adelberg, J. (2005). Efficiency in thin-film liquid system for Hosta micropropagation. Plant Cell, Tissue and Organ Culture, 81(3), 359-368.
• Ari, E., Adelberg, J., Delgado, M., & Kroggel, M. (2015). Selection of the best black mondi (Ophiopogon planiscapus ‘Nigrescens’) clone in tissue culture conditions for micropropagation. Acta Horticulturae, 1087, 423-428.
• Ari, E., Yildirim, T., Mutlu, N., Buyukalaca, S., Gokmen, U., & Akman, E. (2016a). Comparison of different androgenesis protocols for doubled haploid plant production in ornamental pepper (Capsicum annuum L.). Turkish Journal of Biology, 40, 944-954.
• Ari, E., Bedir, H., Yildirim, S., & Yildirim, T. (2016b). Androgenic responses of 64 ornamental pepper (Capsicum annuum L.) genotypes to shed-microspore culture in autumn season. Turkish Journal of Biology, 40, 706-717.
• Bajaj, Y. P. S. (1990). In vitro production of haploids and their use in cell genetics and plant breeding. In Y. P. S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry 12, Haploids in Crop Improvement I (pp.3-44). Berlin: Springer.
• Barroso, P.A., Rego, M.M., Rego, E.R., & Soares, W.S. (2015). Embryogenesis in the anthers of different ornamental pepper (Capsicum annuum L.) genotypes. Genetics and Molecular Research, 14(4), 13349-13363.
• Coleman, A.W., & Goff, L.J. (1985). Applications of fluorochromes to pollen biology. I. Mithramycin and 4′,6-diamidino-2-phenylindole (DAPI) as vital stains and for quantitation of nuclear DNA. Biotechnic and Histochemistry, 60(3), 145-154.
• Custers, J. B. M. (2003). Microspore culture in rapeseed (Brassica napus L.). In M. Maluszynski, K. J. Kasha, B.P. Forster, & I. Szarejko (Eds.), Doubled Haploid Production in Crop Plants A Manual (pp.185-193). Dodrecht: Kluwer Academic Publishers.
• Dias, J. S., & Correia, M. S. (2002). Effect of medium renovation and incubation temperature regimes on tronchuda cabbage microspore culture embryogenesis. Scientia Horticulturae, 93(3-4), 205-214.
• Dolcet-Sanjuan, R., Claveria, E., & Huerta, A. (1997). Androgenesis in Capsicum annuum L. effects of carbohydrate and carbon dioxide enrichment. Journal of the American Society for Horticultural Science, 122(4), 468–475.
• Dumas De Vaulx, R., Chambonnet, D., & Pochard, E. (1981). In vitro culture of pepper (Capsicum annuum L.) anthers: high rate plant production from different genotypes by +35°C treatments haploid and diploid plants, cultivars. Agronomy for Sustainable Development, 1(10), 859–864.
• Ellialtioglu, S., Sari, N., & Abak, K. (2001). Haploid Bitki Uretimi. In M. Babaoglu, E. Gurel, & S. Ozcan, S. (Eds), Bitki Biyoteknolojisi Cilt:I (pp. 138-189). Konya: Selçuk Üniversitesi Vakfı Yayınları.
• Ferrie, A. M. R., Palmer, C. E., & Keller, W.A. (1994). Biotechnological applications of haploids. In P. D. Shargool, & T. T. Ngo (Eds), Biotechnological Applications of Plant Cultures (pp. 77-110). Boca Raton, Florida: CRC Press.
• Ferrie, A. M. R., Palmer, C.E., & Keller, W.A. (1995). Haploid embryogenesis. In T. A. Thorpe (Ed), In vitro Embryogenesis in Plants (pp. 244-309). Dordrecht: Springer.
• Ferrie, A. M. R., & Caswell, K. L. (2011). Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue Organ Culture, 104(3), 301-309.
• George, E. F. (1993). Plant Propagation by Tissue Culture Part 1: The Technology. Exegetics Limited, Edition: 2, UK.
• Kim, M., & Jang, J.C. (2000). Rapid assessment of microspore development stage in pepper using DAPI and ferric chloride. Journal of Plant Biotechnology, 2, 129-134.
• Kim, M., Park, E-J., An, D., & Lee, Y. (2013). High-quality embryo production and plant regeneration using a two-step culture system in isolated microspore cultures of hot pepper (Capsicum annuum L.). Plant Cell Tissue and Organ Culture, 112(2), 191-201.
• Kyte, L., & Kleyn, J. (1996). Plant from test tubes an introduction to micropropagation. Timber Press, Edition: 3, Portland (OR), USA.
• Li, H., & Devaux, P. (2003). High frequency regeneration of barley doubled haploid plants from isolated microspore culture. Plant Science, 164, 379-386.
• Malik, M. R., Wang, F., Dirpaul, J. M., Zhou, N., Hammerlindl, J., Keller, W., Abrams, S. R., Ferrie, A. M. R., & Krochko, J.E. (2008). Isolation of an embryogenic line from non-embryogenic Brassica napus cv. Westar through microspore embryogenesis. Journal of Experimental Botany, 59(10), 2857-2873.
• Maluszynski, M., Kasha, K. J., & Szarejko, I. (2003). Published doubled haploid protocols in plant species. In M. Maluszynski, K. J. Kasha, B.P. Forster, & I. Szarejko (Eds.), Doubled Haploid Production in Crop Plants A Manual (pp. 309-335). Dodrecht: Kluwer Academic Publishers.
• Murovec, J., & Bohanec, B. (2012). Haploids and doubled haploids in plant breeding. In I. Abdurakhmonov (Ed.), Plant Breeding (pp. 87-106). InTech.
• Nitsch, J. P., & Nitsch, C. (1969). Haploid plants from pollen grains. Science, 163(3862), 85–87.
• Palmer, C. E., & Keller, W.A. (1999). Haploidy. In C. Gómez-Campo (Ed.), Biology of Brassica coenospecies (pp. 247-286). Amsterdam: Elsevier.
• Raghuvanshi, S. S., & Srivastava, A. (1995). Plant regeneration of Mangifera indica using liquid shaker culture to reduce phenolic exudation. Plant Cell, Tissue and Organ Culture, 41(1), 83-85.
• Segui-Simarro, J. M. (2010). Androgenesis revisited. The Botanical Review, 76(3), 377-404.
• Segui-Simarro, J. M., Corral-Martinez, P., Parra-Vega, V., & Gonzalez-Garcia, B. (2011). Androgenesis in recalcitrant solanaceous crops. Plant Cell Reports, 30(5), 765-778.
• Smith, M. A. L., & Spomer, L. A. (1995). Vessels, gels, liquid media and support systems. In J. Aitken-Christie, T. Kozai,& M. A. L. Smith (Eds.), Automation and Environmental Control in Plant Tissue Culture (pp. 371-405). Dordrecht: Kluwer Academic Publishers.
• Stommel, J. R., & Bosland, P. W. (2007). Ornamental pepper. In N. O. Anderson (Ed.), Flower Breeding and Genetics - Issues, Challenges and Opportunities for the 21st Century (pp. 561-599). Dordrecht: Springer.
• Supena, E. D. J. (2004). Innovations in microspore embryogenesis in Indonesian hotpepper (Capsicum annuum L.) and Brassica napus L. PhD Thesis, Wageningen University, Wageningen.
• Supena, E. D. J., Muswita, W., Suharsono, S., & Custers, J. B. (2006a). Evaluation of crucial factors for implementing shed-microspore culture of Indonesian hot pepper (Capsicum annuum L.) cultivars. Scientia Horticulturae, 107(3), 226-232.
• Supena, E. D .J., Suharsono, S., Jacobsen, E., & Custers, J. B. (2006b). Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.). Plant Cell Reports, 25(1), 1-10.
• Supena, E. D. J., & Custers, J. B. (2011). Refinement of shed-microspore culture protocol to increase normal embryos production in hot pepper (Capsicum annuum L.). Scientia Horticulturae, 130(4), 769-774.
• Takahashi, S., Matsubara, K., Yamagata, H., & Morimoto, T. (1992). Micropropagation of virus free bulblets of Lilium longiflorum by tank culture. 1. Development of liquid culture method and large scale propagation. Acta Horticulturae, 319(7), 83-88.
• Thomas, T. D. (2008). The role of activated charcoal in plant tissue culture. Biotechnology Advances, 26(6), 618-631.
• Touraev, A., Pfosser, M., & Heberle-Bors, E. (2001). The microspore: A haploid multipurpose cell. Advances in Botanical Research, 35, 53–109.
• Wei, Z., Qiang, F., Xigang, D., & Manzhu, B. (2008). The culture of isolated micropores of ornamental kale (Brassica oleracea var. acephala) and the importance of genotype to embryo regeneration. Scientia Horticulturae, 117(1), 69-72.
• Yang, S., Liu, X., Fu, Y., Zhang, X., Li, Y., Liu, Z., & Feng, H. (2013). The effect of culture shaking on microspore embryogenesis and embryonic development in Pakchoi (Brassica rapa L. ssp. chinensis). Scientia Horticulturae, 152, 70-73.