The Effect of Shaker Use on Embryo Yield in Shed-Microspore Cultures of Ornamental Peppers

Yıl 2019, Cilt: 5 Sayı: 2, 299 - 307, 13.12.2019

Selcen Doğan , Esin Arı

https://doi.org/10.24180/ijaws.627679

Cited By: 1

Öz

Because of their morphological
diversity and various rich content, ornamental peppers (Capsicum annuum L.)
has become a favored plant in ornamental plant, food, cosmetics and medicinal
sectors in recent years. This interest increased the ornamental pepper breeding
studies. However, there are limited numbers of study based on haploid
technology in ornamental peppers. In this study, we aimed to determine the
effect of using shaker on embryo yield in the shed-microspore cultures of
ornamental peppers. We used a total of 29 genotypes and compared the shaken and
stationary cultures for each genotype. The shaken cultures gave more successful
androgenic performance in terms of total and globular embryo formations. The
most successful embryogenic performance was obtained from Genotype 754 in both
stationary and shaken cultures. However, the shaken cultures of Genotype 754
formed 4.5 times more embryos when compared to its stationary control group. As
the result, the use of shaker positively affected embryo yield in
shed-microspore cultures of ornamental pepper depending on genotype.

Anahtar Kelimeler

Capsicum annuum L., doubled haploid, microspore embryogenesis, culture shaking

Destekleyen Kurum

Scientific and Technological Research Council of Turkey

Proje Numarası

5120019-TUBITAK-TEYDEB-1505

Çalkalayıcı Kullanımının Süs Biberi Shed-Mikrospor Kültürlerinde Embriyo Verimine Etkisi

Öz

Morfolojik çeşitlilikleri ve
çeşitli zengin içerikleri nedeniyle süs biberleri (Capsicum annuum L.) son yıllarda süs bitkisi, gıda, kozmetik ve
ilaç sektörlerinde tercih edilen bitkiler haline gelmiştir. Süs biberine olan
bu ilgi süs biberinde yapılan ıslah çalışmalarını son yıllarda arttırmıştır.
Ancak süs biberlerinde haploid teknolojisine dayalı sınırlı sayıda ıslah
çalışması bulunmaktadır. Bu çalışmada, süs biberi shed-mikrospor kültürlerinde
çalkalayıcı kullanımının embriyo verimi üzerine etkisi belirlenmeye
çalışılmıştır. Toplam 29 süs biberi genotipi kullanılmış ve her genotipin
çalkalanan ve durağan kültürleri karşılaştırılmıştır. Çalkalanan kültürler,
toplam ve globular embriyo oluşturma açısından daha başarılı androjenik
performans göstermiştir. Çalışmada en başarılı embriyojenik performans, hem
durağan hem çalkalanan kültürlerde Genotip 754'ten elde edilmiştir. Genotip
754'ün çalkalanan kültürleri, durağan kontrol grubuna kıyasla 4.5 kat daha
fazla embriyo oluşturmuştur. Sonuç olarak, çalkalayıcı kullanımı, genotipe
bağlı olarak süs biberi shed-mikrospor kültürlerinde embriyo verimini olumlu
yönde etkilemiştir. 

Anahtar Kelimeler

Anter, Capsicum annuum L., doubled haploid, mikrospor embriyogenesis, çalkalama

Proje Numarası

5120019-TUBITAK-TEYDEB-1505

Kaynakça

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