Aktaş Yoncası (Melilotus alba Desr.) Genotiplerinin Sekonder Metabolit İçeriği Bakımından Değerlendirilmesi

Year 2021, Volume: 7 Issue: 3, 524 - 532, 30.12.2021

İlknur Yıldırım , Yasin Emre Öztürk , Yusuf Murat Kardeş , Erdem Gülümser , Hanife Mut

https://doi.org/10.24180/ijaws.936893

Cited By: 1

Abstract

Baklagiller familyasında yer alan taş yoncaları içermiş olduğu ikincil metabolitler ile hayvan besleme ve sağlığı açısından önem teşkil etmektedir. Diğer taraftan, kıraç ve fakir topraklarda kurulacak kısa süreli meraların kalitesinin artırılmasında kullanılan taş yoncalarının yem kalitesi yüksek olmakla birlikte, yonca ve üçgül türlerine göre şişme problemi daha azdır. Bu araştırma, Bilecik ili doğal florasından toplanan 17 adet aktaş yoncası genotipinin (Melilotus alba Desr.) toplam fenolik, toplam flavonoid, radikal kovucu aktivite (DPPH) ve kondanse tanen içeriklerinin belirlenmesi amacı ile yapılmıştır. Araştırma sonucuna göre, genotiplerin ortalama fenolik, flavonoid, DPPH ve kondanse tanen içerikleri sırasıyla 3.901 mg GA g-1, 17.962 mg QE g-1, %26.523 ve %0.611 olarak belirlenmiş olup, söz konusu bileşikler bakımından aktaş yoncası genotipleri arasında farklılıklar olmuştur. Ayrıca, çalışmada belirlenen bu bileşikler yem kalitesi ve hayvan sağlığı açısından çok önemli olup, sonuçlar ileride yapılacak çalışmalara ışık tutacak niteliktedir.

Keywords

Aktaş yoncası, genotip, yem bitkisi, antioksidan, kondanse tanen.

Supporting Institution

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Project Number

Başvuru numarası: 1919B012002821 ve 1919B011903472

Thanks

Bu çalışmaya maddi destek sağlayan, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)’na teşekkür ederiz. (2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı (Başvuru numarası: 1919B012002821 ve 1919B011903472).

Evaluation of White Sweet Clover (Melilotus alba Desr.) Genotypes in terms of Secondary Metabolite Contents.

Abstract

Sweet clovers are belonging to the fabaceae and are important for animal nutrition and health in terms of the secondary metabolites it contains. Besides, sweet clovers increase the quality of short-term pastures to be established in arid and poor soils, and their feed quality is high and the swelling problem is less than compared to the alfalfa and trefoil species. In this study, it was aimed to determine the total phenolic (TP), total flavonoid (TF), radical scavenging activity (DPPH) and condensed tannin contents (CT) of 17 white sweet clover (Melilotus alba Desr.) collected from the natural flora of Bilecik. As a result, the average TP, TF, DPPH, and CT contents of white sweet clover genotypes were determined as 3.901 mg GA g-1 17.962 mg QE g-1, 26.523%, and 0.611%, respectively, and there were differences between the white sweet clover genotypes in terms of these compounds. Besides, these compounds determined in the study are very important in terms of feed quality and animal health, and the results will shed light on future studies.

Keywords

White sweet clover, genotype, forage crop, antioxidant, condensed tannin.

Project Number

Başvuru numarası: 1919B012002821 ve 1919B011903472

References

• Ahmadipour, B, Hassanpour, H., Asadi, E., Khajali, F., Rafiei, F., & Khajali, F. (2015) Kelussia odoratissima Mozzaf– A promising medicinal herb to prevent pulmonary hypertension in broiler chickens reared at high altitude. Journal of Ethnopharmacology, 159, 49-54.
• Ahmadipour, B., Kalantar, M., Hosseini, S. M., Yang, L. G., Kalantar, M. H., Raza, S. H. A., & Schreus, N. M. (2017) Hawthorn (Crataegus oxyacantha) Extract in the Drinking Water of Broilers on Growth and Incidence of Pulmonary Hypertension Syndrome (PHS). Brazilian Journal of Poultry Science, 19(4), 639-644.
• Al-Snafi, A, E. (2020). Phenolics and flavonoids contents of medicinal plants, as natural ingredients for many therapeutic purposes- A review. The International Organization of Scientific Research Journal Of Pharmacy, 10(7), 42-81.
• Anonim. (2021). Bilecik ili haritası. https://tr.pinterest.com/pin/629941066623287479/. Erişim tarihi: 10 Mayıs 2021. Arvouet-Grand, A., Vennat, B., Pourrat, A., & Legret, P. (1994). Standardisation d`un extrait de propolis et identification des principaux constituants. Journal de pharmacie de Belgique, 49, 462-468.
• Bal, M. A., Ozturk, D., Aydin, R., Erol, A., Ozkan, C. O., Ata, M., Karakas, E., & Karabay, P. (2006). Nutritive value of sainfoin (Onobrychis viciaefolia) harvested at different maturity stages. Journal of Biological Science, 9, 205-209.
• Barry, T. N. (1987). Secondary compounds of forages. In J. B. Hacker, & J. H. Ternouth (Eds.), Nutrition of Herbivores (pp. 91-120). Sydney, Academic Press.
• Bate-Smith, E. C. (1975). Phytochemistry of proanthocyanidins. Phytochemistry, 14, 1107-1113.
• Dohi, H., Yamada, A., & Fukukawa, T. (1997). Intake stimulants in perennial ryegrass (Lolium perenne L.) fed to sheep. Journal of Dairy Science, 80, 2083–2086.
• Faller, A., & Fialho, E. (2009). The antioxidant capacity and polyphenol content of organic and conventional retail vegetables after domestic cooking. Food Research International, 42, 210-215.
• Frozza, C. O. S., Garcia, C. S. C., Gambato, G., De Souza, M. D., Salvador, M., Moura, S., Padilha, F. F., Seixas, F. K., Collares, T., Borsuk, S., Dellagostin, O. A., Henriques, J. A., & Roesch-Ely, M. (2013). Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis. Food and Chemical Toxicology, 52, 137-142.
• Heydari, H., Saltan, G., Bahadır Açıkara, Ö., Yılmaz, S., Çoban, T., & Tekin, M. (2015). Antioxidant activity of five Lathyrus L. species growing in Turkey. Turkish Journal of Pharmaceutical Sciences, 12(3), 369-376.
• Kuhnen, S., Moacyr, J. R., Mayer, J. K., Navarro, B. B., Trevisan, R., Honorato, L. A., Maraschin, M., & Pinheiro Machado Filho, L. C. (2014). Phenolic content and ferric reducing-antioxidant power of cow’s milk produced in different pasture-based production systems in southern Brazil. Journal of the Science of Food and Agriculture, 94, 3110–3117.
• Kökten, K., Kaplan, M., Turan, V., Kale, H., Çaçan, E., Kardeş, Y. M., Tutar, H., & Tal, E. (2017). Farklı meşe palamudu türlerinin (Quercus sp.) hayvan besleme özellikleri. 12. Tarla Bitkileri Kongresi, Kahramanmaraş.
• Kumar, R., & Singh, M. (1984). Tannins: Their adverse role in ruminant nutrition. Journal of Agricultural and Food Chemistry, 32, 447-453.
• Kumar, S., & Pandey, A. K. (2013) Chemistry and Biological Activities of Flavonoids: An Overview. The Scientific World Journal, 162750.
• Lee, S. H. Y., Humphries, D. J., Cockman, D. A., Givens, D. I., & Spencer, J. P. E. (2017). Accumulation of citrus flavanones in bovine milk following citrus pulp incorporation into the diet of dairy cows. EC Nutrition, 7(4), 143-154.
• Lascano, C. E., & Cárdenas, E. (2010). Alternatives for methane emission mitigation in livestock systems. Revista Brasileira de Zootecnia, 39, 175-182.
• Lüscher, A., Suter, M., & Finn, J. A. (2016). Legumes and grasses in mixtures complement each other ideally for sustainable forage production. The journal of the International Legume Society, 12, 8-10.
• Mammadov, R. (2014). Tohumlu Bitkilerde Sekonder Metabolitler. Nobel Akademik Yayıncılık ve Eğitim Danışmanlık Ticaret Limited Şirketi Yayınları, Yayın No: 20779, Ankara.
• Martin, C., Copani, G., & Niderkorn, V. (2016). Impacts of forage legumes on intake, digestion and methane emissions in ruminants. The journal of the International Legume Society, 12, 24-25.
• Mladenović, K. G., Muruzović., M. Ž., Stefanović, O. D., Vasic Ljiljana, S. M., & Čomić, R. (2016). Antımıcrobıal, Antioxidant and Antibiofilm Activity of Extracts of Melilotus Officinalis (L.) Pall. The Journal of Animal & Plant Sciences, 26(5), 1436-1444.
• Mueggler, W. F., & Stewart, W. L. (1978). Grassland and shrubland habitat types of western Montana. Gen. Tech. Rep. INT-66. Ogden, UT: U.S. Department of Agriculture, Forest Service, Intermountain Forest and Range Experiment Station.
• O’Connell, J. E., & Fox, P. F. (2001). Significanceandapplicationsofphenoliccompounds in the production and quality of milk and dairy products: areview. International Dairy Journal, 11, 103–120.
• Patra, A. K., Kamra, D. N., & Agarwal, N. (2006). Effect of plant extracts on in vitro methanogenesis, enzyme activities and fermentation of feed in rumen liquor of buffalo. Animal Feed Science and Technology, 128(3-4), 276–291.
• Paula, E. M., Samensari, R. B., Machado, E., Pereira, L. M., & Maia, F. J. (2016). Effects of phenolic compounds on ruminal protozoa population, ruminal fermentation, and digestion in water buffaloes. Livestock Science, 185, 136-41.
• Robbins, R. J. (2003). Phenolic acids in foods. Journal of Agricultural and Food Chemistry, 51, 2866–2887. Rochfort, S., Parker, A. J., & Dunshea, F. R. (2008). Plant bioactives for ruminant health and productivity. Phytochemistry, 69(2), 299–322.
• Santos Neto, T. M., Mota, R. A., Silva, L. B. G., Viana, D. A., Lima-Filho, J. L., Sarubbo, L. A., Converti, A., & Porto, A. L. F. (2009). Susceptibility of Staphylococcus spp. isolated from milk of goats with mastitis to antibiotics and green propolis extracts. Letters in Drug Design & Discovery, 6, 63-68.
• Seradj, A. R., Abecia, L., Crespo, J., Villalba, D., Fondevila, M., & Balcells, J. (2014). The effect of bioflavex and its pure flavonoid components on in vitro fermentation parameters and methane production in rumen fluid from steers given high concentrate diets. Animal Feed Science and Technology, 197, 85-91.
• Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
• Sonju, J. J., Islam, M. D. F., Sutradhar, K., & Akter, T. (2017). Analysıs of phytochemical, antioxidant and microbial property of various extracts of the plant melilotus Indica. World Journal of Pharmaceutical Research, 6(4), 29-47.
• Tan, A. (1992). Türkiye’deki bitkisel çeşitlilik ve bitki genetik kaynakları. Anadolu Dergisi, 2, 50–64.
• Wheeler, T., & Reynolds, C. (2013). Predicting the risks from climate change to forage and crop production for animal feed. Animal Frontiers, 1, 36–41.
• Xiao, J., Kai, G., Yamamoto, K., & Chen, X. (2013) Advance in dietary polyphenolsas α-glucosidases inhibitors: a review on structure-activity relationship aspect. Critical Reviews in Food Science and Nutrition, 53(8), 818-836.
• Xing-zhou, T., Paengkoum, P., Paengkoum, S., Thongpea, S., & Chao, B. (2018). Journal of Integrative Agriculture, 17(9), 2082–2095.
• Yıldız, B., Öztürk, Y. E., Kardeş, Y. M., Mut, H., & Gülümser, E. (2021). Kaba Yem olarak değerlendirilen ökse otunun antioksidan özellikleri ve kondanse tanen içeriklerinin belirlenmesi. Anadolu Tarım Bilimleri Dergisi, 36, 132-137.
• Zhan, J., Liu, M., Su, X., Zhan, K., Zhang, C., & Zhao, G. (2017). Effects of alfalfa flavonoids on the production performance, immune system, and ruminal fermentation of dairy cows. Archive of Asian-Australasian Journal of Animal Sciences, 30(10), 1416-1424.