Chemical Vapor Deposition of Poly(hydroxyethyl methacrylate-glycidyl methacrylate) Thin Film Coatings for Immobilization of Human Serum Albumin

Year 2019, Volume: 3 Issue: 2, 96 - 119, 16.12.2019

Fatma Sarıipek Esra Çağıl Mustafa Karaman

Abstract

In this study, poly(2-hydroxyethyl methacrylate-glycidyl
methacrylate) (P(HEMA-GMA) thin films were deposited on crosslinked polyvinyl
alcohol (PVA) supports by initiated chemical vapor deposition (iCVD). Use of
the tert-butyl peroxide as an initiator allowed very high deposition rates up
to 125 nm/min at a filament temperature of 280 ^oC. FTIR and XPS
analyses of the deposited films confirmed that the epoxide functionality of the
copolymers increased with increasing glycidyl methacrylate fraction in the reactor
inlet. The potential of the glycidyl methacrylate containing copolymer films to
act as substrates for protein immobilization was revealed. The immobilization
of Human Serum Albumin (HSA), which is the main protein to carry fatty acids
and metals to target tissues, was carried out via solid phase extraction. The
effects of film composition and thickness on binding capacities of the protein
to the polymers were studied. The maximum protein binding for the iCVD
synthesized copolymer films was found to be 223 µg.cm^-2. Protein
binding was also clarified by FTIR, AFM, and SEM analyses. The mild
immobilization conditions, easy and rapid membrane preparation, one-step
protein binding at substantially higher levels and membrane reusability make
iCVD deposited   P(HEMA-GMA) films useful
for biomolecules immobilization and for several biochemical processes.

Keywords

iCVD, Thin film hydrogel membrane, Protein immobilization, P(HEMA-GMA), Crosslinked PVA

Project Number

BAP-12101019).

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