Year 2018,
Volume: 4 Issue: 2, 47 - 52, 01.10.2018
Gizem Bayatbalay
Z. Dilek Heperkan
References
- [1] TGK, (2009). Türk Gıda Kodeksi, Fermente
Süt Ürünleri Tebliği, Madde 4. Tarım ve Köy İşleri
Bakanlğı, Tebliğ No:2009/25)
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Süt Ürünleri Tebliği, Madde 4. Tarım ve Köy İşleri
Bakanlğı, Tebliğ No:Taslak/2017.
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& Heperkan, D. (2013). A review on traditional
Turkish fermented non-alcoholic beverages:
Microbiota, fermentation process and quality
characteristics. International Journal of Food
Microbiology. 167: 44–56.
- [4] Sabir, F., Beyatli, Y., Cokmus, C. & Onal-Darilmaz,
D., (2010). Assessment of potential probiotic
properties of Lactobacillus spp., Lactococcus
spp., and Pediococcus spp. Strains isolated from
kefir. Journal of Food Science. 75: 568-573.
- [5] Kesmen, Z & Kacmaz, N. (2011). Determination
of lactic microflora of kefir grains and kefir
beverage by using culture‐dependent and cultureindependent
methods. Food Science. 76 (5):
M276-M283
- [6] Leroy, F. & De Vuyst, L. (2004). Lactic acid
bacteria as functional starter cultures for the food
fermentation industry. Trends in Food Science and
Technology. 15: 67-78.
- [7] Simova, E., Beshkova, D., Angelov, A.,
Hristozova, Ts, Frengova, G. & Spasov, Z., (2002).
Lactic acid bacteria and yeasts in kefir grains
and kefir made from them. Journal of Industrial
Microbiology and Biotechnology. 28: 1-6.
- [8] Powell, J. E., Witthuhn, R. C., Todorov, S.
D., & Dicks, L. M. T. (2007). Characterization
of bacteriocin ST8KF produced by a kefir isolate
Lactobacillus plantarum ST8KF. International
Dairy Journal, 17(3), 190-198.
- [9] Wang, J., Zhao, X., Tian, X. Z., He, C., Yang, Y.
& Yang, Z. (2015). isolation and characterization
of exopolysaccharide-producing Lactobacillus
plantarum SKT109 from Tibet kefir. Polish Journal
of Food and Nutritional Sciences. 65: 269-279.
- [10] Witthuhn, R. C., Schoeman, T., & Britz,
T. J. (2005). Characterisation of the microbial
population at different stages of kefir production
and kefir grain mass cultivation. International
Dairy Journal, 15(4), 383-389.
- [11] Magalhaes, K.T., Pereira, G.V.M., Campos,
C.R., Dragone, G. & Schwan, R.F. (2011).
Brazilian kefir: structure, microbial communities
and chemical composition. Brazilian Journal of
Microbiology. 42: 693-702.
- [12] Wang, S., Chen, K., Lo, Y., Chiang, M.,
Chen, H. & Liu, J. (2012). Investigation of microorganisms
involved in biosynthesis of the kefir
grain. Food Microbiology. 32: 274-285.
- [13] Renner, E. & Renz, S. (1986). Nahrewettabellen
fur milch und milchprodukte. Verlag B. Renner,
Köhler K. G. Gieben, Germany.
- [14] Alpkent Z. & Küçükçetin A. (2003). Farklı
sıcaklıklarda muhafaza edilen kefirlerin duyusal,
fiziksel, kimyasal ve mikrobiyolojik özelliklerinde
meydana gelen değişmeler. VI. Süt ve Süt Ürünleri
Sempozyumu, Tekirdağ, Türkiye, 22-23 Mayıs,
p.363-373
- [15] Karagözlü C. (2003). Kefir-probiotic fermented
milk product, 50th Anniversary of The University
of Food Teknology HIFFI 15-17 Oct. Collection of
Scientific Works of The HIFFI Plovdiv, Bulgaria.
50: 404-409.
- [16] Chen H. C., Wang S. Y. & Chen M. J.
(2008). Microbiological study of lactic acid bacteria
in kefir grains by culture-dependent and cultureindependent
methods. Food Microbiology. 25:
492–501.
DETERMINATION OF THE PRESENCE OF LIVING MICROORGANISMS IN KEFIR GRAIN
Year 2018,
Volume: 4 Issue: 2, 47 - 52, 01.10.2018
Gizem Bayatbalay
Z. Dilek Heperkan
Abstract
Kefir, defined as fermented milk product, is a drink that lactic acid bacteria and yeasts formed together with kefir grain obtained by adding milk and waiting at room temperature for one day. Kefir is being investigated with increasing interest in recent years as it contains micro-organisms useful for health. Although kefir is produced on an industrial scale, consumers have traditionally been producing kefir in homes. This study aimed to determine the presence of living lactic acid bacteria and yeast in kefir grain. Man Rogosa and Sharp (MRS) media were used for isolation and counting of lactic acid bacteria and Yeast extract Glucose Chloramphenicol agar (YGC) was used for yeasts. Kefir grain was found to contain 5x104 CFU/g of living lactic acid bacteria and yeast.
References
- [1] TGK, (2009). Türk Gıda Kodeksi, Fermente
Süt Ürünleri Tebliği, Madde 4. Tarım ve Köy İşleri
Bakanlğı, Tebliğ No:2009/25)
- [2] TGK, (2017). Türk Gıda Kodeksi, Fermente
Süt Ürünleri Tebliği, Madde 4. Tarım ve Köy İşleri
Bakanlğı, Tebliğ No:Taslak/2017.
- [3] Altay, F., Karbancıoglu-Güler, F., Daskaya, C.
& Heperkan, D. (2013). A review on traditional
Turkish fermented non-alcoholic beverages:
Microbiota, fermentation process and quality
characteristics. International Journal of Food
Microbiology. 167: 44–56.
- [4] Sabir, F., Beyatli, Y., Cokmus, C. & Onal-Darilmaz,
D., (2010). Assessment of potential probiotic
properties of Lactobacillus spp., Lactococcus
spp., and Pediococcus spp. Strains isolated from
kefir. Journal of Food Science. 75: 568-573.
- [5] Kesmen, Z & Kacmaz, N. (2011). Determination
of lactic microflora of kefir grains and kefir
beverage by using culture‐dependent and cultureindependent
methods. Food Science. 76 (5):
M276-M283
- [6] Leroy, F. & De Vuyst, L. (2004). Lactic acid
bacteria as functional starter cultures for the food
fermentation industry. Trends in Food Science and
Technology. 15: 67-78.
- [7] Simova, E., Beshkova, D., Angelov, A.,
Hristozova, Ts, Frengova, G. & Spasov, Z., (2002).
Lactic acid bacteria and yeasts in kefir grains
and kefir made from them. Journal of Industrial
Microbiology and Biotechnology. 28: 1-6.
- [8] Powell, J. E., Witthuhn, R. C., Todorov, S.
D., & Dicks, L. M. T. (2007). Characterization
of bacteriocin ST8KF produced by a kefir isolate
Lactobacillus plantarum ST8KF. International
Dairy Journal, 17(3), 190-198.
- [9] Wang, J., Zhao, X., Tian, X. Z., He, C., Yang, Y.
& Yang, Z. (2015). isolation and characterization
of exopolysaccharide-producing Lactobacillus
plantarum SKT109 from Tibet kefir. Polish Journal
of Food and Nutritional Sciences. 65: 269-279.
- [10] Witthuhn, R. C., Schoeman, T., & Britz,
T. J. (2005). Characterisation of the microbial
population at different stages of kefir production
and kefir grain mass cultivation. International
Dairy Journal, 15(4), 383-389.
- [11] Magalhaes, K.T., Pereira, G.V.M., Campos,
C.R., Dragone, G. & Schwan, R.F. (2011).
Brazilian kefir: structure, microbial communities
and chemical composition. Brazilian Journal of
Microbiology. 42: 693-702.
- [12] Wang, S., Chen, K., Lo, Y., Chiang, M.,
Chen, H. & Liu, J. (2012). Investigation of microorganisms
involved in biosynthesis of the kefir
grain. Food Microbiology. 32: 274-285.
- [13] Renner, E. & Renz, S. (1986). Nahrewettabellen
fur milch und milchprodukte. Verlag B. Renner,
Köhler K. G. Gieben, Germany.
- [14] Alpkent Z. & Küçükçetin A. (2003). Farklı
sıcaklıklarda muhafaza edilen kefirlerin duyusal,
fiziksel, kimyasal ve mikrobiyolojik özelliklerinde
meydana gelen değişmeler. VI. Süt ve Süt Ürünleri
Sempozyumu, Tekirdağ, Türkiye, 22-23 Mayıs,
p.363-373
- [15] Karagözlü C. (2003). Kefir-probiotic fermented
milk product, 50th Anniversary of The University
of Food Teknology HIFFI 15-17 Oct. Collection of
Scientific Works of The HIFFI Plovdiv, Bulgaria.
50: 404-409.
- [16] Chen H. C., Wang S. Y. & Chen M. J.
(2008). Microbiological study of lactic acid bacteria
in kefir grains by culture-dependent and cultureindependent
methods. Food Microbiology. 25:
492–501.