Analysis of WRKY Transcription Factors in Barley Cultivars Infected with Fusarium culmorum

Year 2019, , 165 - 174, 28.12.2019

Ebru Uluhan Esra Nur Keleş Feyza Tufan

https://doi.org/10.38001/ijlsb.588730

Cited By: 13

Abstract

One of the most critical problems of cereal breeding
is Fusarium crown rot disease caused
by various Fusarium species. Fusarium culmorum is one of the
predominant pathogen in Turkey and causes serious product losses. In this
study, the early response of barley cultivars upon F. culmorum infection were analyzed by disease severity and gene
expression patterns of WRKY transcription factors. In that context, firstly,
disease severities of 9 barley cultivars (Hordeum
vulgare L. cvs. Epona, Escadre, Gazda, Oliver, Avcı 2002, Burakbey, Tarm
92, Manava, and Ramata) infected with F.
culmorum were determined with disease index percentages. After 7 days of
infection, Epona was more sensitive than the other cultivars while the lowest
disease index was seen in Gazda. Total RNA extractions were performed at 72 hai
from the root tissues of Epona and Gazda. Expression analysis of HvWRKY6, HvWRKY9, HvWRKY24, HvWRKY25, HvWRKY33, HvWRKY34, HvWRKY42, and HvWRKY46 genes were
conducted by qPCR. As a result of pathogen stress, it was observed that the
transcript levels of HvWRKY33 was
significantly upregulated in both cultivars. HvWRKY6, HvWRKY34 and HvWRKY46 genes were increased in Epona
while upregulation of HvWRKY25 and HvWRKY34 genes were detected in Gazda.
No significant decreases were detected in any cultivars. This study is
important in terms of providing an association between WRKY genes and pathogen stress response.

Keywords

Barley, Fusarium culmorum, Fusarium crown rot, WRKY

Supporting Institution

TUBITAK

Project Number

1919B011702097

Thanks

The authors thank TUBITAK for their financial support to conduct this research.

Fusarium culmorum ile Enfekte Edilen Arpa Çeşitlerinde WRKY Transkripsiyon Faktörlerinin Analizi

Abstract

Project Number

1919B011702097

References

• Durrant W.E., Dong X Systemic acquired resistance. Annu Rev Phytopathol, 2004. 42: p. 185–209
• Montesano, M., Brader, G., and Palva, E.T., Pathogen derived elicitors: searching for receptors in plants. Molecular Plant Pathology, 2003. 4(1): p.73-79.
• van Loon, L.C., M. Rep and C.M. Pieterse, Significance of inducible defense-related proteins in infected plants. Annual Review of Phytopathology., 2006. 44: p. 135-162.
• Nejat, N., N. Mantri, Plant immune system: Crosstalk between responses to biotic and abiotic stresses the missing link in understanding plant defence. Signal, 2017. 2: p. 2.
• Satapathy, L., D. Kumar, and K. Mukhopadhyay, WRKY transcription factors: Involvement in plant–pathogen interactions. In Recent advances in Applied Microbiology Springer, Singapore, 2017. p. 229-246.
• Moreno, J. E., G. Moreno-Piovano, and R. L. Chan, The antagonistic basic helix-loop-helix partners BEE and IBH1 contribute to control plant tolerance to abiotic stress. Plant Science, 2018. 271: p. 143-150.
• Welner, D. H., et al., NAC transcription factors: from structure to function in stress-associated networks. In Plant Transcription Factors, 2016. p. 199-212.
• Alves, M., et al., Plant bZIP transcription factors responsive to pathogens: a review. International Journal of Molecular Sciences, 2013. 14(4): p. 7815-7828.
• Jiang, J., et al., WRKY transcription factors in plant responses to stresses. Journal of İntegrative Plant Biology, 2017. 59(2): p. 86-101.
• Lehti-Shiu, Melissa D., et al., Diversity, expansion, and evolutionary novelty of plant DNA-binding transcription factor families. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 2017. 1860(1): p. 3-20.
• Chen, F., et al., The WRKY transcription factor family in model plants and crops. Critical Reviews in Plant Sciences, 2017. 36(5-6): p. 311-335.
• Phukan, U.J., G.S. Jeena, and R.K. Shukla, WRKY transcription factors: molecular regulation and stress responses in plants. Frontiers in Plant Science, 2016. 7: p. 760.
• Yörük, E., et al., Salinity and drought stress on barley and wheat cultivars planted in Turkey. Journal of Environmental Biology, 2018. 39(6): p. 943-950.
• Rushton P.J., I.E.Somssich, Transcriptional control of plant genes responsive to pathogens. Curr Opin Plant Biology. 1998; 1(4): p. 311–315.
• Zheng Z, et al., Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens. Plant Journal, 2006. 48: p.592–605.
• Eulgem T., I.E. Somssich, Networks of WRKY transcription factors in defense signaling. Curr Opin Plant Biology, 2007. 10(4): p.366–371
• van Verk, M.C., et al., A novel WRKY transcription factor is required for induction of PR-1a gene expression by salicylic acid and bacterial elicitors. Plant Physiology, 2008. 146(4): p. 1983-1995.
• Nakayama A. et al., Genome-wide identification of WRKY45-regulated genes that mediate benzothiadiazole-induced defense responses in rice. BMC plant biology, 2013. 13(1): p. 150.
• Mangelsen, E., et al., Phylogenetic and comparative gene expression analysis of barley (Hordeum vulgare) WRKY transcription factor family reveals putatively retained functions between monocots and dicots. BMC Genomics, 2008. 9(1): p. 194.
• Bentley, A.R., et al., A survey of Fusarium species associated with wheat and grass stem bases in northern Turkey, Sydowia, 2006. 58: p. 163-177.
• Tunalı, B., et al., Root and crown rot fungi associated with spring, facultative, and winter wheat in Turkey. Plant Disease, 2008. 92: p. 1299–1306.
• Matny, O.N., Fusarium head blight and crown rot on wheat & barley: losses and health risks. Advances in Plants & Agriculture Research, 2015. 2(1): p. 39.
• Wagacha, J.M., J.W. Muthomi, Fusarium culmorum: Infection process, mechanisms of mycotoxin production and their role in pathogenesis in wheat. Crop protection, 2007. 26(7): p. 877-885.
• Covarelli, L., et al., Fusarium Virulence Assay on Wheat and Barley Seedlings. Bio-protocol, 2013. 3(7): p. 446.
• Ferdous, J., et al., Identification of reference genes for quantitative expression analysis of MicroRNAs and mRNAs in barley under various stress conditions. PLoS One, 2015. 10(3), e0118503.
• Wang H., et al., Production and characterization of antifungal compounds produced by Lactobacillus plantarum IMAU10014. PLoS One, 2012. 7(1): p. 1-7.
• Liu, Y., et al., Genotypic differences to crown rot caused by Fusarium pseudograminearum in barley (Hordeum vulgare L.). Plant Breeding, 2012. 131. p. 728–732.
• Liu, C., F.C. Ogbonnaya, Resistance to Fusarium crown rot in wheat and barley: a review. Plant Breeding, 2015. 134: p. 365– 372.
• Beccari et al., Infection processes and soft wheat response to root rot and crown rot caused by Fusarium culmorum, Plant pathology, 2011. 60,: p. 671-684.
• Gardiner et al., An ABC pleiotropic drug resistance transporter of Fusarium graminearum with a role in crown and root diseases of wheat, FEMS microbiology letters, 2013. 348: p. 36-45.
• Warzecha, T., E. Skrzypek, and A. Sutkowska, Effect of Fusarium culmorum infection on selected physiological and biochemical parameters of barley (Hordeum vulgare L.) DH lines, Physiological and molecular plant pathology, 2015. 89: p. 62-69.
• Tufan, F., et al., Analysis of early events in barley (Hordeum vulgare L.) roots in response to Fusarium culmorum infection. European Journal of Plant Pathology, 2017. 148(2): p. 343-355.
• Birkenbihl, R. P., S. Liu, and I.E. Somssich, Transcrip- tional events defining plant immune responses. Curr Opin Plant Biology, 2017. 38: p. 1–9.
• Li, H., et al., Comparative expression analysis of five WRKY genes from Tibetan hulless barley under various abiotic stresses between drought-resistant and sensitive genotype. Acta Physiologiae Plantarum, 2014. 36(4): p. 963-973.
• Meng, Y., W.P. Roger, HvWRKY10, HvWRKY19, and HvWRKY28 regulate Mla-triggered immunity and basal defense to barley powdery mildew. Molecular Plant-Microbe Interactions, 2012. 25(11): p. 492-1505.
• Liu, D., et al., Phylogenetic analysis of barley WRKY proteins and characterization of HvWRKY1 and-2 as repressors of the pathogen-inducible gene HvGER4c. Molecular Genetics and Genomics, 2014. 289(6): p. 1331-1345.
• Karre, S., et al., HvWRKY23 regulates flavonoid glycoside and hydroxycinnamic acid amide biosynthetic genes in barley to combat Fusarium head blight. Plant Molecular Biology, 2019. p. 1-15.
• Gao J., et al., WRKY Transcription Factors Associated With NPR1-Mediated Acquired Resistance in Barley Are Potential Resources to Improve Wheat Resistance to Puccinia triticina. Frontiers in Plant Science, 2018. 9:1486.
• Peng, Xi-xu, et al., Isolation and expression patterns of rice WRKY82 transcription factor gene responsive to both biotic and abiotic stresses. Agricultural Sciences in China, 2011. 10(6): p. 893-901.