Anticholinergic, Antioxidant Activity and LC-MS/MS analysis of Ethanol Extract from Salvia officinalis L.

Abstract

Medicinal plants have many biological activities because they contain important phenolic and flavonoid compounds that can be used in new drug development. Therefore, it is important to know the contribution of phenolic content of plants to bioactivities. In this study, the phenolic content of Salvia officinalis L. ethanol (SOLE) extract was determined with LC-MS/MS. The anticholinergic and antioxidant (metal reduction, radical and lipid peroxidation removal) activities of the extract in vitro were determined by methods specified in the material method. The SOLE extract showed an inhibition effect on AChE enzyme (IC50: 0.136 mg / ml). Moreover, the plant extract showed 17 % DPPH radical removal activity, while 22 % ABTS showed radical removal activity. The SOLE extract (10 μg / mL) inhibited lineloic acid oxidation by 24 %. The results showed that this plant contributes to antioxidant defense systems against oxidative stress. As a result, the medicinal plant, which has significant bioactivity due to its acetylcholinesterase inhibitory effect and antioxidant activities, can be used in the treatment of many diseases such as atherosclerosis, Alzheimer's

Keywords

• Sage,lipid peroxidation,antioxidants,acetylcholinesterase,phytochemical analysis

Salvia officinalis L. Etanol Ekstraktının Antikolinerjik ve Antioksidan Aktivitesi ve LC-MS/MS Analizi

Abstract

Tıbbi bitkiler, yeni ilaç gelişiminde kullanılabilecek önemli fenolik ve flavonoid bileşikleri içerdiklerinden dolayı birçok biyolojik aktiviteye sahiptir. Bu yüzden bitkilerin fenolik içeriğinin biyoaktivitelere katkısının bilinmesi önem arzetmektedir. Bu çalışmada, Salvia officinalis L. etanol (SOLE) ekstresinin fenolik içeriği LC-MS/MS ile belirlendi. Bu ektrenin in vitro olarak, antikolinerjik ve antioksidan (metal indirgeme, radikal ve lipit peroksidasyon giderme) aktiviteleri materyal metotta belirtilen yöntemlerle belirlendi. The SOLE ektresi AChE enzimi üzerine (IC50: 0.136 mg / ml) inhibisyon etkisi göstermiştir. Dahası, bu bitki ektresi % 17 oranında DPPH radikal giderme aktivitesi gösterirken, % 22 oranında ABTS radikal giderme aktivitesi göstermiştir. The SOLE (10 μg/mL) ektresi lineloik asit oksidasyonunu % 24 inhibe etmiştir. Bu çıktılar oksidatif strese karşı bu bitkinin antioksidan savunma sistemlerine katkıda bulunduğunu göstermektedir. Sonuç olarak, asetilkolinesteraz inhibitör etkisi ve antioksidan aktiviteleri nedeniyle önemli biyoaktiviteye sahip olan bu tıbbi bitki ateroskleroz, Alzheimer gibi birçok hastalıkların tedavisinde kullanılabilir.

Keywords

• Adaçayı,Fitokimyasal analiz,Antioksidan,Asetilkolinesteraz,Lipit peroksidasyonu

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