Farklı Benzil Amino Pürin ve Gibberellik Asit Kombinasyonunun Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne’nın Mikroçoğaltımı Üzerine Etkisi

Year 2019, Volume: 2 Issue: 2, 72 - 79, 12.08.2019

Muhammet Doğan

https://doi.org/10.38001/ijlsb.562130

Cited By: 1

Abstract

Rotala rotundifolia (Buch-Ham. ex Roxb)
Koehne geneneksel
tıp sisteminde hastalıkların tedavisinde kullanılan önemli akuatik tıbbi
bitkidir. Bu çalışmada, 6-benzil amino purin (BAP) ve gibberellik asit (GA₃)’in
farklı kombinasyonlarını içeren kültür ortamında R. rotundifolia’nın boğum eksplnatlarından in vitro sürgün rejenerasyonu araştırılmıştır. Kültür ortamlarında
ilk sürgün oluşumları 10 gün sonunda gözlenmiştir. Sekiz hafta sonunda deneme
sonlandırılmış ve rejenerasyon verileri alınmıştır. Sürgün rejenerasyon oranı %72,22-100,00 arasında sıralanmıştır. %100
sürgün rejenerasyon oranı 0,25-0,75 mg/L BAP + 0,25 mg/L GA₃ içeren
MS ortamında kaydedilmiştir. Eksplant başına maksimum sürgünler (18,38 adet) ve
en uzun sürgünler (2,36 cm) 0,25 mg/L BAP + 0,25 mg/L GA₃ eklenmiş
kültürlerde elde edilmiştir. Çoğaltım ortamında sürgünlerin köklenmesi
nedeniyle ayrıca köklendirme çalışması yürütülmemiştir. Rejenere sürgünlerin
akvaryum ortamına alıştırılması başarıyla sağlanmıştır. Bu rapor, tıbbi bitki R. rotundifolia’nın çoklu üretimini
sunması bakımından önemlidir. İleride bu bitki ile yürütülecek sekonder
metabolit üretimi ve gen aktarım çalışmalarına yardımcı olabilir.

Keywords

Boğum eksplantı, Doku kültürü, Sürgün rejenerasyonu, BAP

Supporting Institution

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Project Number

2130190

Thanks

Bu çalışma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) tarafından 2130190 numaralı proje ile desteklenmiştir. Desteklerinden dolayı TÜBİTAK'a teşekkür ederim.

The Effect of Different Combinations of Benzyl Amino Purine and Gibberellic Acid on Micropropagation of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Abstract

Rotala rotundifolia (Buch-Ham. ex Roxb)
Koehne is an important aquatic medicinal plant for the treatment of diseases in
the traditional medicine system. In this study, in vitro shoot regeneration from the
nodal explants of R. rotundifolia in
culture medium containing different combinations of 6-benzyl amino purine (BAP)
and gibberellic acid (GA₃) was investigated. First shoot formation
was observed after 10 days in culture medium. At the end of eight weeks, the
trial was completed and regeneration data were obtained. Shoot regeneration rate is listed as
72.22-100.00%. 100% shoot regeneration rate was recorded in MS medium including
0.25-0.75 mg/L BAP + 0.25 mg/L GA₃. The maximum shoots per explant (18.38) and the
longest shoots (2.36 cm) were obtained in cultures with 0.25 mg/L BAP + 0.25
mg/L GA₃. No rooting study was carried out due to the rooting of the
shoots in the multiplication environment. The acclimation of regenerated shoots
to the aquarium environment has been successfully achieved. This report is
important in that it offers multiple production of medicinal plant R. rotundifolia. In the future, it can
help with the production of secondary metabolites and gene transfer studies.

Keywords

Nodal explant, Tissue culture, Shoot regeneration, BAP

Project Number

2130190

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