Farklı Benzil Amino Pürin ve Gibberellik Asit Kombinasyonunun Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne’nın Mikroçoğaltımı Üzerine Etkisi

Yıl 2019, Cilt: 2 Sayı: 2, 72 - 79, 12.08.2019

Muhammet Doğan

https://doi.org/10.38001/ijlsb.562130

Cited By: 1

Öz

Rotala rotundifolia (Buch-Ham. ex Roxb)
Koehne geneneksel
tıp sisteminde hastalıkların tedavisinde kullanılan önemli akuatik tıbbi
bitkidir. Bu çalışmada, 6-benzil amino purin (BAP) ve gibberellik asit (GA₃)’in
farklı kombinasyonlarını içeren kültür ortamında R. rotundifolia’nın boğum eksplnatlarından in vitro sürgün rejenerasyonu araştırılmıştır. Kültür ortamlarında
ilk sürgün oluşumları 10 gün sonunda gözlenmiştir. Sekiz hafta sonunda deneme
sonlandırılmış ve rejenerasyon verileri alınmıştır. Sürgün rejenerasyon oranı %72,22-100,00 arasında sıralanmıştır. %100
sürgün rejenerasyon oranı 0,25-0,75 mg/L BAP + 0,25 mg/L GA₃ içeren
MS ortamında kaydedilmiştir. Eksplant başına maksimum sürgünler (18,38 adet) ve
en uzun sürgünler (2,36 cm) 0,25 mg/L BAP + 0,25 mg/L GA₃ eklenmiş
kültürlerde elde edilmiştir. Çoğaltım ortamında sürgünlerin köklenmesi
nedeniyle ayrıca köklendirme çalışması yürütülmemiştir. Rejenere sürgünlerin
akvaryum ortamına alıştırılması başarıyla sağlanmıştır. Bu rapor, tıbbi bitki R. rotundifolia’nın çoklu üretimini
sunması bakımından önemlidir. İleride bu bitki ile yürütülecek sekonder
metabolit üretimi ve gen aktarım çalışmalarına yardımcı olabilir.

Anahtar Kelimeler

Boğum eksplantı, Doku kültürü, Sürgün rejenerasyonu, BAP

Destekleyen Kurum

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Proje Numarası

2130190

Teşekkür

Bu çalışma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) tarafından 2130190 numaralı proje ile desteklenmiştir. Desteklerinden dolayı TÜBİTAK'a teşekkür ederim.

The Effect of Different Combinations of Benzyl Amino Purine and Gibberellic Acid on Micropropagation of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Öz

Rotala rotundifolia (Buch-Ham. ex Roxb)
Koehne is an important aquatic medicinal plant for the treatment of diseases in
the traditional medicine system. In this study, in vitro shoot regeneration from the
nodal explants of R. rotundifolia in
culture medium containing different combinations of 6-benzyl amino purine (BAP)
and gibberellic acid (GA₃) was investigated. First shoot formation
was observed after 10 days in culture medium. At the end of eight weeks, the
trial was completed and regeneration data were obtained. Shoot regeneration rate is listed as
72.22-100.00%. 100% shoot regeneration rate was recorded in MS medium including
0.25-0.75 mg/L BAP + 0.25 mg/L GA₃. The maximum shoots per explant (18.38) and the
longest shoots (2.36 cm) were obtained in cultures with 0.25 mg/L BAP + 0.25
mg/L GA₃. No rooting study was carried out due to the rooting of the
shoots in the multiplication environment. The acclimation of regenerated shoots
to the aquarium environment has been successfully achieved. This report is
important in that it offers multiple production of medicinal plant R. rotundifolia. In the future, it can
help with the production of secondary metabolites and gene transfer studies.

Anahtar Kelimeler

Nodal explant, Tissue culture, Shoot regeneration, BAP

Proje Numarası

2130190

Kaynakça

• 1. Bhowmik S, M. Saha, and B.K. Datta, Extended Distribution of Rotala rotundifolia (Buch.-Ham. ex Roxb.) Koehne (Lyhthraceae) from India. NeBIO, 2012. 3: p. 48-50.
• 2. Tan, Q.G., et al., Megastigmane-type Compounds from Rotala rotundifolia. Chinese Journal of Natural Medicines, 2009. 7: p. 187-189.
• 3. Ho, Y.L., et al., In vitro Antioxidant Properties and Total Phenolic Contents of Wetland Medicinal Plants in Taiwan. Bot Stud, 2012. 53: p. 55-66.
• 4. Cirik, Ş., S. Cirik, ve M. Conk-Dalay, Su Bitkileri II (İçsu Bitkilerinin Biyolojisi, Ekolojisi, Yetiştirme Teknikleri). E.Ü. Su Ürünleri Fakültesi Yayınları, No:61, 2011. 4-150 s, İzmir.
• 5. Sivanesan, I. et al., Micropropagation of Cotoneaster wilsonii Nakai-a Rare Endemic Ornamental Plant. Plant Cell, Tissue and Organ Culture (PCTOC), 2011. 105: p. 55-63.
• 6. Joshi, P., and V. Dhawan, Axillary Multiplication of Swertia chirayita (Roxb. Ex Fleming) H. Karst., a Critically Endangered Medicinal Herb of Temperate Himalayas. In Vitro Cellular & Developmental Biology-Plant, 2007. 43(6): p. 631-638.
• 7. Sivanesan, I., and B.R. Jeong, Direct Shoot Regeneration from Nodal Explants of Sida cordifolia Linn. In Vitro Cellular & Developmental Biology-Plant, 2007. 43(5): p. 436-441.
• 8. Offord, C.A., and J.L. Tyler, In vitro Propagation of Pimelea spicata R. Br (Thymelaeaceae), an Endangered Species of the Sydney Region, Australia. Plant Cell, Tissue and Organ Culture (PCTOC), 2009. 98(1): p. 19-23.
• 9. Gonçalves, S., L. Fernandes, and A. Romano, High-Frequency In Vitro Propagation of the Endangered Species Tuberaria Major. Plant Cell, Tissue and Organ Culture (PCTOC), 2010. 101(3): p. 359-363.
• 10. Murashige, T., and F. Skoog, A Revised Medium For Rapid Growth And Bioassays With Tobacco Tissue Cultures. Physiological Plantarum, 1962. 15: p. 473-497.
• 11. Snedecor, G.W., and W.G. Cochran, Statistical Methods. The Iowa State University Press, 1997. Iowa, USA.
• 12. Mishra, A.K., et. al., Factors Affecting the Efficiency of In Vitro Regeneration from Seedling-Derived Nodal Explants of Nyctanthes arbor-tristis L. and Evaluation of Genetic Fidelity. Plant Biosystems, 2019. p. 1-9.
• 13. Dogan, M., Multiple Shoot Regeneration Via Indirect Organogenesis from Shoot Tip and Nodal Meristem Explants of Ceratophyllum demersum L. Journal of Animal and Plant Sciences, 2019. 29(2): p. 568-577.
• 14. Reddy, R., M. Nakhooda, and S. Shaik, Germination Studies And In Vitro Propagation of Momordica balsamina L. Using nodal explants and zygotic embryos. South African Journal of Botany, 2018. 115: p. 328-328.
• 15. Subbaiyan, B., M. Visveshwari, and V. Thangapandian, An Efficient Indirect Regeneration And Multiple Shoots Formation from Nodal Explant of Ceropegia juncea Roxb. International Journal of Conservation Science, 2017. 8(3): p. 529-536.
• 16. Warakagoda, P. S., S. Subasinghe, and M.T.K Gunasekare,. In vitro Clonal Propagation of Coscinium fenestratum (Gertn.) Colebr.(Weniwel) Through Nodal Explants. Journal of the National Science Foundation of Sri Lanka, 2017. 45(2): p. 133-141.
• 17. Emsen, B. and M. Dogan, Evaluation of Antioxidant Activity of In Vitro Propagated Medicinal Ceratophyllum demersum L. Extracts. Acta Scientiarum Polonorum-Hortorum Cultus, 2018. 17(1): p. 23-33.
• 18. Dogan, M., In Vitro Shoot Regeneration of Limnophila aromatica (Lamk.) Merr. from Nodal and Internodal Explants. Iğdır Universiry Journal of the Institue of Science and Technology, 2018. 8(3): p. 77-84.
• 19. Acemi, A., Comparative Analysis Of The Effects of Chitosan And Common Plant Growth Regulators on In Vitro Propagation of Ipomoea purpurea (L.) Roth from Nodal Explants. In Vitro Cellular & Developmental Biology-Plant, 2018. 54(5): p. 537-544.
• 20. Dogan, M., In Vitro Micropropagation from Nodal Explants of the Medicinal Plant Lysimachia nummularia L.. KSU Journal of Agriculture and Nature, 2018. 21(6): p. 875-881.
• 21. Enyew, M., and T. Feyissa, In Vitro Shoot Regeneration from Leaf Explants of Echinops kebericho: an Endangered Endemic Medicinal Plant. Plant Biosystems, 2019. 153(2): p. 199-204.
• 22. Tan, S.N., C.S. Tee, and H.L. Wong, Multiple Shoot Bud İnduction and Plant Regeneration Studies of Pongamia pinnata. Plant Biotechnology, 2018. 35: p. 325-334.
• 23. Varis, S., K., Klimaszewska, and T. Aronen, Somatic Embryogenesis and Plant Regeneration from Primordial Shoot Explants of Picea abies (L.) H. Karst. Somatic Trees. Frontiers in plant science, 2018. 9: 1551.
• 24. Dogan, M., In Vitro Rapid Propagation Of An Aquatic Plant Pogostemon erectus (Dalzell) Kuntze. Anatolian Journal of Botany, 2019. 3(1): p. 1-6.
• 25. Dogan, M., Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Anatolian Journal of Botany, 2017. 1(1,2): p. 4-8.