Analysis of WRKY Transcription Factors in Barley Cultivars Infected with Fusarium culmorum

Year 2019, Volume: 2 Issue: 3, 165 - 174, 28.12.2019

Ebru Uluhan Esra Nur Keleş Feyza Tufan

https://doi.org/10.38001/ijlsb.588730

Cited By: 13

Abstract

One of the most critical problems of cereal breeding
is Fusarium crown rot disease caused
by various Fusarium species. Fusarium culmorum is one of the
predominant pathogen in Turkey and causes serious product losses. In this
study, the early response of barley cultivars upon F. culmorum infection were analyzed by disease severity and gene
expression patterns of WRKY transcription factors. In that context, firstly,
disease severities of 9 barley cultivars (Hordeum
vulgare L. cvs. Epona, Escadre, Gazda, Oliver, Avcı 2002, Burakbey, Tarm
92, Manava, and Ramata) infected with F.
culmorum were determined with disease index percentages. After 7 days of
infection, Epona was more sensitive than the other cultivars while the lowest
disease index was seen in Gazda. Total RNA extractions were performed at 72 hai
from the root tissues of Epona and Gazda. Expression analysis of HvWRKY6, HvWRKY9, HvWRKY24, HvWRKY25, HvWRKY33, HvWRKY34, HvWRKY42, and HvWRKY46 genes were
conducted by qPCR. As a result of pathogen stress, it was observed that the
transcript levels of HvWRKY33 was
significantly upregulated in both cultivars. HvWRKY6, HvWRKY34 and HvWRKY46 genes were increased in Epona
while upregulation of HvWRKY25 and HvWRKY34 genes were detected in Gazda.
No significant decreases were detected in any cultivars. This study is
important in terms of providing an association between WRKY genes and pathogen stress response.

Keywords

Barley, Fusarium culmorum, Fusarium crown rot, WRKY

Supporting Institution

TUBITAK

Project Number

1919B011702097

Thanks

The authors thank TUBITAK for their financial support to conduct this research.

Fusarium culmorum ile Enfekte Edilen Arpa Çeşitlerinde WRKY Transkripsiyon Faktörlerinin Analizi

Abstract

Project Number

1919B011702097

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