Ege Üniversitesi Tıp Fakültesi Hastanesinde Toksoplazmozis Sıklığının Real-Time PZR ile Belirlenmesi

Year 2022, Volume: 8 Issue: 1, 217 - 223, 30.06.2022

Muhammet Karakavuk Hüseyin Can Aysu Değirmenci Döşkaya Adnan Gürüz Mert Döşkaya

https://doi.org/10.29132/ijpas.1099082

Abstract

Toxoplasma gondii insan ve sıcakkanlı hayvanları enfekte edebilen zoonotik protozoon bir parazittir. Bu çalışmada çeşitli hasta gruplarından alınmış klinik örneklerde toksoplazmozis sıklığının araştırılması amaçlanmıştır. Çalışma kapsamında 2009-2019 yılları arasında Ege Üniversitesi Tıp Fakültesi Parazitoloji Anabilim Dalı Moleküler Parazitoloji laboratuvarına gönderilen ağırlıklı olarak amniyon sıvısı, kan ve beyin omurilik sıvısı örneklerinin dahil olduğu toplam 535 klinik örnek değerlendirilmiştir. Bu örneklerde DNA izolasyonu sonrası T. gondii RE geni varlığı Real-Time PZR ile araştırılmıştır.
Belirtilen zaman aralığında toksoplazmozis sıklığı %2,61 (14/535) olarak tespit edilmiştir. Hastalığın direkt
etkilediği merkezi organlardan alınan örneklerde pozitiflik oranı %5,40 (8/148) iken periferden alınan örneklerde pozitiflik oranı %1,74 (5/286) olarak tespit edilmiş ve bu fark istatistiksel olarak anlamlı bulunmuştur (P<0,05). İmmunsüpresif hastalarda ise hastalığın pozitiflik oranı daha yüksek olmasına rağmen istatistiksel olarak anlamlı bir fark saptanmamıştır (P>0,05).
Elde edilen bulgular toksoplazmozis tanısında Real-Time PZR yönteminin oldukça önemli bir yeri olduğunu
göstermektedir. Ayrıca, örnek alım yöntemlerinin hastalığın tanısında oldukça önemli olduğu sonucuna varılmıştır.

Keywords

Toxoplasma gondii, toksoplazmozis, RT-PZR, RE geni

Supporting Institution

Ege Üniversitesi

Project Number

13-TIP-050

Thanks

Bu çalışma Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 13-TIP-050 numaralı proje ile desteklenmiştir

Determination of Toxoplasmosis Frequency by Real-Time PCR in Ege University Medical Faculty Hospital

Abstract

Toxoplasma gondii is a zoonotic protozoan parasite that can infect humans and warm-blooded animals. In this study, it was aimed to investigate the frequency of toxoplasmosis in clinical samples taken from various patient groups. Within the scope of the study, a total of 535 clinical samples, including mainly amniotic fluid, blood and cerebrospinal fluid samples, which were sent to the Molecular Parasitology laboratory of Ege University Faculty of Medicine, Department of Parasitology between 2009 and 2019, were evaluated.
The frequency of toxoplasmosis was determined as 2.61% (14/535) in the specified time period. While the positivity rate was 5.40% (8/148) in the samples taken from the central organs directly affected by the disease, the positivity rate was 1.74% (5/286) in the samples taken from the periphery, and this difference was statistically significant (P<0.05). Although the positivity rate of the disease was higher in immunosuppressive patients, no statistically significant difference was found (P>0.05).
The findings show that Real-Time PCR method has a very important place in the diagnosis of active toxoplasmosis. In addition, it was concluded that sampling methods are very important in the diagnosis of the disease.

Keywords

RT-PCR, Toxoplasma gondii, toxoplasmosis, RT-PCR, RE gene

Project Number

13-TIP-050

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