Research Article
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Year 2020, Volume: 2 Issue: 2, 62 - 71, 26.08.2020
https://doi.org/10.38058/ijsl.764947

Abstract

References

  • 1. Ali,G., Hadi,F., Ali,Z., Tariq,M. and Khan,M.A.(2007). Callus induction and in vitro complete plant regeneration of different cultivars of tobacco (Nicotiana tobacum L.) on media of different hormonal concentration. Journal of Biotechnology Vol.6 (4), 561-566
  • 2. Andrey S. Marchev and Milen I. Georgiev(2020). Plant In Vitro Systems as a Sustainable Source of Active Ingredients for Cosmeceutical Application. Molecules , 25, 2006; doi:10.3390/molecules25082006
  • 3. Arvas, Y.E., H.M. Aksoy, and Y. Kaya, Patates Bitkisinde Biyoteknolojik Çalışmalar. International Journal of Life Sciences and Biotechnology, 2018. 1(1): p. 37-47.
  • 4. Deo, P.C., Tyagi, A.P., Taylor, M., Harding, R. And Becker, D. 2010. Factors affecting somatic embryogenesis and transformation in modern plant breeding. South Pac. J. Nat. App. Sci, 28:27–40.
  • 5. Ganasan K. and Fahrul Huyop (2010) In vitro regeneration of Citrullus lanatus cv. Round Dragon. Journal of Biological Sciences. 10(2):131-137. ISSN 1727-3048.
  • 6. Ganapathi TR, Suprasanna P, Rao PS, Bapat VA. Tobacco (Nicotiana tabacum L.) - A model system for tissue culture interventions and genetic engineering. Indian J Biotechnol 2004;3:171-84.
  • 7. Jawad, Z.A., Turker, M. and Ozdemir, F.A. 2020. Effect of different plant growth regulator on in vitro propagation of endangered plant; yellow tomato (Lycopersicon esculentum Mill.). Int. J. Agric. For. Life Sci., 4(1): 92-98.
  • 8. Kaya, Y., in vitro Plant Regeneration of Tobacco. , in Faculty of Biosciences and Bioengineering. 2010, Universiti Teknologi Malaysia: Malaysia.
  • 9. Kaya Y, S. Yilmaz, S. Marakli, N. Gozukirmizi and F. Huyop, “Transformation of Nicotiana tabacum with DehE Gene,” Journal of Food Agriculture and Environment, Vol. 11, No. 3-4, 2013
  • 10. Kaya, Y. and S. Karakütük, Farklı büyüme düzenleyicilerin Türk kır çeltiği rejenerasyonuna etkisi. Anadolu Tarım Bilimleri Dergisi, 2018. 33(3): p. 226-231.
  • 11. Kocacaliskan, İ., T. Akgul, and S. Erisen, Effect of Juglone on Seed Germination and Seedling Growth of Four Common Vegetables. International Journal of Life Sciences and Biotechnology, 2019. 2(1): p. 43-49.
  • 12. Kutty P.C., G.K.A. Parveez and F. Huyop (2011) Agrobacterium tumefaciens –infection strategies for greater transgenic recovery in Nicotiana tabacum cv. TAPM26. International Journal of Agricultural Research 6(2):119-113. ISSN 1816-4897
  • 13. Kutty P.C., G.K.A. Parveez and F. Huyop (2010) An easy method for Agrobacterium tumefaciens –mediated gene transfer to Nicotiana tabacum cv. TAPM26. Journal of Biological Sciences 10(6):480-489. ISSN 1727-3048.
  • 14. Kumar, N. and M.P. Reddy (2010). Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Annals of Applied Biology. 156: 367-375
  • 15. Latifian, E., Y.E. Arvas, and Y. Kaya, Tagetes minuta Bitkisinin Mikro Üretimi Üzerine Farklı BAP ve IAA Konsantrasyonlarının Etkileri. International Journal of Life Sciences and Biotechnology, 2018.1(2): p. 96-104.
  • 16. Mohammed S. Effects and Quantity Ranges Of Some Auxins On Embryogenic Callus Induction From Upland Rice Cultivars: An Overview. International Journal of Life Sciences and Biotechnology. 2020. DOI: https://doi.org/10.38001/ijlsb.669914
  • 17. Ozyigit ıı, M. V. Kahraman, O. Ercan, (2007), Relation between explant age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.), Afr J Biotechnol, 6(1), 3-8,
  • 18. Ozyigit i ibrahim, (2008), Phenolic changes during in vitro organogenesis of cotton (Gossypium hirsutum L.) shoot tips, Afr J Biotechnol, 7(8), 1145-1150
  • 19. Steyn Anita E. , J. A. Brink & L. A. Coetzer (1997) A comparison of culture methods for tobacco (Nicotianatabacum) anthers, South African Journal of Plant and Soil, 14:1, 27-30, DOI: 10.1080/02571862.1997.10635076
  • 20. Taha A.M., A. Wagiran, H. Ghazali, F. Huyop and G.K.A. Parveez (2009). In Vitro Regeneration of Garden Balsam, Impatiens balsamina using cotyledons derived from seedlings. Biotechnology. 8(1):44-52. ISSN 1682-296X.

An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26

Year 2020, Volume: 2 Issue: 2, 62 - 71, 26.08.2020
https://doi.org/10.38058/ijsl.764947

Abstract

In vitro regeneration of Nicotiana tabacum was efficiently achieved using growth regulators combination supplemented into semi-solid MS medium using leaves from 1-month-old plant as explants. The growth regulators used were NAA for auxin (0.0, 1.0, 2.0, and 3.0 mgL-1) and BAP (0.0, 0.5, 1.0, 2.5, and 5.0 mgL-1). The effects of growth regulator combination were assessed based on a number of callus formation, shoots formation and fresh callus weight. The maximum number of callus formation was 100 % at five hormone combination, observed at the fourth week after culture. Maximum number of shoots produced per explant was 21.4 shoots at 1.0 mgL-1 BAP + 3.0 mgL-1 NAA, after 6 weeks of culture. The maximum callus fresh weights were obtained at 0.5 mgL-1 BAP + 1.0 mgL-1 NAA after 6 weeks (9.92 g). The best combination for shoots regeneration of Nicotiana tabacum was 1.0 mgL-1 BAP + 3.0 mgL-1 NAA. And the best combination for heaviest callus production was 0.5 mgL-1 BAP + 1.0 mgL-1 NAA.

References

  • 1. Ali,G., Hadi,F., Ali,Z., Tariq,M. and Khan,M.A.(2007). Callus induction and in vitro complete plant regeneration of different cultivars of tobacco (Nicotiana tobacum L.) on media of different hormonal concentration. Journal of Biotechnology Vol.6 (4), 561-566
  • 2. Andrey S. Marchev and Milen I. Georgiev(2020). Plant In Vitro Systems as a Sustainable Source of Active Ingredients for Cosmeceutical Application. Molecules , 25, 2006; doi:10.3390/molecules25082006
  • 3. Arvas, Y.E., H.M. Aksoy, and Y. Kaya, Patates Bitkisinde Biyoteknolojik Çalışmalar. International Journal of Life Sciences and Biotechnology, 2018. 1(1): p. 37-47.
  • 4. Deo, P.C., Tyagi, A.P., Taylor, M., Harding, R. And Becker, D. 2010. Factors affecting somatic embryogenesis and transformation in modern plant breeding. South Pac. J. Nat. App. Sci, 28:27–40.
  • 5. Ganasan K. and Fahrul Huyop (2010) In vitro regeneration of Citrullus lanatus cv. Round Dragon. Journal of Biological Sciences. 10(2):131-137. ISSN 1727-3048.
  • 6. Ganapathi TR, Suprasanna P, Rao PS, Bapat VA. Tobacco (Nicotiana tabacum L.) - A model system for tissue culture interventions and genetic engineering. Indian J Biotechnol 2004;3:171-84.
  • 7. Jawad, Z.A., Turker, M. and Ozdemir, F.A. 2020. Effect of different plant growth regulator on in vitro propagation of endangered plant; yellow tomato (Lycopersicon esculentum Mill.). Int. J. Agric. For. Life Sci., 4(1): 92-98.
  • 8. Kaya, Y., in vitro Plant Regeneration of Tobacco. , in Faculty of Biosciences and Bioengineering. 2010, Universiti Teknologi Malaysia: Malaysia.
  • 9. Kaya Y, S. Yilmaz, S. Marakli, N. Gozukirmizi and F. Huyop, “Transformation of Nicotiana tabacum with DehE Gene,” Journal of Food Agriculture and Environment, Vol. 11, No. 3-4, 2013
  • 10. Kaya, Y. and S. Karakütük, Farklı büyüme düzenleyicilerin Türk kır çeltiği rejenerasyonuna etkisi. Anadolu Tarım Bilimleri Dergisi, 2018. 33(3): p. 226-231.
  • 11. Kocacaliskan, İ., T. Akgul, and S. Erisen, Effect of Juglone on Seed Germination and Seedling Growth of Four Common Vegetables. International Journal of Life Sciences and Biotechnology, 2019. 2(1): p. 43-49.
  • 12. Kutty P.C., G.K.A. Parveez and F. Huyop (2011) Agrobacterium tumefaciens –infection strategies for greater transgenic recovery in Nicotiana tabacum cv. TAPM26. International Journal of Agricultural Research 6(2):119-113. ISSN 1816-4897
  • 13. Kutty P.C., G.K.A. Parveez and F. Huyop (2010) An easy method for Agrobacterium tumefaciens –mediated gene transfer to Nicotiana tabacum cv. TAPM26. Journal of Biological Sciences 10(6):480-489. ISSN 1727-3048.
  • 14. Kumar, N. and M.P. Reddy (2010). Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Annals of Applied Biology. 156: 367-375
  • 15. Latifian, E., Y.E. Arvas, and Y. Kaya, Tagetes minuta Bitkisinin Mikro Üretimi Üzerine Farklı BAP ve IAA Konsantrasyonlarının Etkileri. International Journal of Life Sciences and Biotechnology, 2018.1(2): p. 96-104.
  • 16. Mohammed S. Effects and Quantity Ranges Of Some Auxins On Embryogenic Callus Induction From Upland Rice Cultivars: An Overview. International Journal of Life Sciences and Biotechnology. 2020. DOI: https://doi.org/10.38001/ijlsb.669914
  • 17. Ozyigit ıı, M. V. Kahraman, O. Ercan, (2007), Relation between explant age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.), Afr J Biotechnol, 6(1), 3-8,
  • 18. Ozyigit i ibrahim, (2008), Phenolic changes during in vitro organogenesis of cotton (Gossypium hirsutum L.) shoot tips, Afr J Biotechnol, 7(8), 1145-1150
  • 19. Steyn Anita E. , J. A. Brink & L. A. Coetzer (1997) A comparison of culture methods for tobacco (Nicotianatabacum) anthers, South African Journal of Plant and Soil, 14:1, 27-30, DOI: 10.1080/02571862.1997.10635076
  • 20. Taha A.M., A. Wagiran, H. Ghazali, F. Huyop and G.K.A. Parveez (2009). In Vitro Regeneration of Garden Balsam, Impatiens balsamina using cotyledons derived from seedlings. Biotechnology. 8(1):44-52. ISSN 1682-296X.
There are 20 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Yilmaz Kaya 0000-0003-1506-7913

Fahrul Zaman Huyop 0000-0003-3978-4087

Publication Date August 26, 2020
Published in Issue Year 2020 Volume: 2 Issue: 2

Cite

APA Kaya, Y., & Huyop, F. Z. (2020). An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26. International Journal of Science Letters, 2(2), 62-71. https://doi.org/10.38058/ijsl.764947
AMA Kaya Y, Huyop FZ. An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26. IJSL. August 2020;2(2):62-71. doi:10.38058/ijsl.764947
Chicago Kaya, Yilmaz, and Fahrul Zaman Huyop. “An Easy and Reliable Method for Establishment and Maintenance of Tissue Cultures of Nicotiana Tabacum Cv TAPM 26”. International Journal of Science Letters 2, no. 2 (August 2020): 62-71. https://doi.org/10.38058/ijsl.764947.
EndNote Kaya Y, Huyop FZ (August 1, 2020) An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26. International Journal of Science Letters 2 2 62–71.
IEEE Y. Kaya and F. Z. Huyop, “An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26”, IJSL, vol. 2, no. 2, pp. 62–71, 2020, doi: 10.38058/ijsl.764947.
ISNAD Kaya, Yilmaz - Huyop, Fahrul Zaman. “An Easy and Reliable Method for Establishment and Maintenance of Tissue Cultures of Nicotiana Tabacum Cv TAPM 26”. International Journal of Science Letters 2/2 (August 2020), 62-71. https://doi.org/10.38058/ijsl.764947.
JAMA Kaya Y, Huyop FZ. An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26. IJSL. 2020;2:62–71.
MLA Kaya, Yilmaz and Fahrul Zaman Huyop. “An Easy and Reliable Method for Establishment and Maintenance of Tissue Cultures of Nicotiana Tabacum Cv TAPM 26”. International Journal of Science Letters, vol. 2, no. 2, 2020, pp. 62-71, doi:10.38058/ijsl.764947.
Vancouver Kaya Y, Huyop FZ. An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26. IJSL. 2020;2(2):62-71.