Antioxidant and Antimicrobial Properties of Cistus Ladanifer

Year 2017, Volume: 4 Issue: 3, Special Issue 2, 434 - 444, 20.12.2017

Merve Deniz Köse , Buse Nur Tekin Oğuz Bayraktar Emre Taylan Duman Yücel Başpınar

https://doi.org/10.21448/ijsm.376834

Cited By: 7

Abstract

Different parts of Cistus species are traditionally used in folk medicine as a remedy for several microbial disorders and infections. At the beginning of the study, crude extract of the Cistus leaves was obtained to determine its antioxidant and antimicrobial activities. Then volatile compounds were extracted using hydrodistillation and hexane. The Cistus oil was obtained by hydrodistillation of fresh material, using leaves in an altered Clevenger-type device. Aqueous extract of Cistus leaves was neutralized to precipitate gums. The yield of gum was determined as 1%. Resinoid was obtained after ethanol extraction of gum. Antibacterial activities of extract were determined using disc diffusion and micro-dilution assays against gram-positive and gram-negative bacteria. The extract of Cistus leaves tested and exhibited antibacterial activities by inhibiting one or more microorganisms. The tested plant extract was more active against gram-positive bacteria compared with gram-negative bacteria. Total phenol content of the extract was determined with Folin-ciocalteu method. Total phenolic content of Cistus extract was 520 ± 15 mg Gallic Acid Equivalent (GAE)/ g extract. The water soluble (ACW) and lipid soluble (ACL) antioxidant capacities of the extract were also determined. ACW and ACL antioxidant capacities of Cistus extract were found as 650±80 μg Ascorbic acid/ mg extract and 540 ±30 μg Trolox Equivalent/ mg extract, respectively. In this study, there is a clear relationship between the total antioxidant and phenol content analysis results and antibacterial activities. Hereby, bioactive natural compounds present in Cistus species can be used as natural raw material in some related industrial applications.

Keywords

Cistus genus, Antibacterial activity, Antioxidant capacity, Labdanum

Antioxidant and Antimicrobial Properties of Cistus Ladanifer

Abstract

Different parts of Cistus
species are traditionally used in folk medicine as a remedy for several microbial
disorders and infections. At the beginning of the study, crude extract of the Cistus leaves was obtained to determine its
antioxidant and antimicrobial activities. Then volatile compounds were extracted
using hydrodistillation and hexane. The Cistus
oil was obtained by hydrodistillation of fresh material, using leaves in an altered
Clevenger-type device. Aqueous extract of Cistus
leaves was neutralized to precipitate gums. The yield of gum was determined as 1%.
Resinoid was obtained after ethanol extraction of gum. Antibacterial activities
of extract were determined using disc diffusion and micro-dilution assays against
gram-positive and gram-negative bacteria. The extract of Cistus leaves tested and exhibited antibacterial activities by inhibiting
one or more microorganisms. The tested plant extract was more active against gram-positive
bacteria compared with gram-negative bacteria. Total phenol content of the extract
was determined with Folin-ciocalteu method. Total phenolic content of Cistus extract was 520 ± 15 mg Gallic Acid
Equivalent (GAE)/ g extract. The water soluble (ACW) and lipid soluble (ACL) antioxidant
capacities of the extract were also determined. ACW and ACL antioxidant capacities
of Cistus extract were found as 650±80
μg Ascorbic acid/ mg extract and 540 ±30 μg Trolox Equivalent/ mg extract, respectively.
In this study, there is a clear relationship between the total antioxidant and phenol
content analysis results and antibacterial activities. Hereby, bioactive natural
compounds present in Cistus species can
be used as natural raw material in some related industrial applications.

Keywords

Cistus genus, Antibacterial activity, Antioxidant capacity, Labdanum

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