PROPOLİS VE PERGANIN ANTİMİKROBİYAL ETKİLERİNİN İN-VİTRO OLARAK ARAŞTIRILMASI

Year 2021, , 1084 - 1093, 29.11.2021

Feyzi Doğru , Hakan Parlakpınar Yücel Duman , Onural Özhan Merve Keskin , Alaadin Polat

https://doi.org/10.33715/inonusaglik.942345

Cited By: 1

Abstract

Propolis, arıların bitkilerin çeşitli kısımlarından topladığı reçine benzeri doğal bir maddedir. Arılar propolisi çeşitli patojenlere karşı koruyucu olarak kullanırlar. Propolis zengin içeriği sayesinde birçok biyolojik etkiye sahiptir. Perga, arıların yavrularını beslemek için kullandığı polen ve baldan oluşan fermente bir üründür. Bu çalışmanın amacı fenolik bileşiklerce zengin olan perganın, antimikrobiyal aktivitesinin propolisle karşılaştırılmasıdır. Propolis ve perganın etanolik ekstraktları hazırlandı ve Folin-Ciocalteu yöntemi ile toplam fenolik bileşik miktar tayini yapıldı. Ekstraktların gram-negatif patojenler olan Escherichia coli ve Pseudomonas aeruginosa, gram-pozitif bir patojen olan Staphylococcus aureus ve bir mantar türü olan Candida albicans üzerinde minimum inhibitör konsantrasyon (MİK) değerleri mikrodilüsyon yöntemi ile ölçüldü. Propolis ve perga için MİK test aralığı fenolik bileşik miktarına göre 0,06µg/mL-125µg/mL olarak belirlendi. Propolis, Escherichia coli ve Pseudomonas aeruginosa’ya göre Candida albicans ve Staphylococcus aureus’a karşı düşük konsantrasyonlarda inhibe edici etki göstermiştir. Perganın Escherichia coli, Pseudomonas aeruginosa ve Candida albicans’a karşı yine düşük konsantrasyonda inhibitör etki gösterdiği; ancak, perganın Staphylococcus aureus’a karşı inhibitör etkinlik gösterebilmesi için çok daha yüksek konsantrasyonlara ihtiyaç olduğu saptanmıştır. Sonuç olarak propolis ve perganın çalışmamızda antimikrobiyal etki gösterdiği izlenmiştir.

Keywords

Anti-enfektif ajanlar, Apiterapi, Mikrobiyal duyarlılık testleri, Propolis

Investigation of In-vitro Antimicrobial Effects of Propolis and Perga

Abstract

Propolis is a resin like natural material that bees collect from various parts of plants. Bees use propolis as protective against various pathogens. Propolis has many biological effects due to its rich content. Perga is a fermented product of pollen and honey that bees use to feed their offspring. The aim of this study is to compare the perga, which is rich by phenolic compounds, to the propolis for its antimicrobial activity.Ethanolic extracts of the propolis and perga were prepared and total phenolic compound quantity determination were made according to the Folin-Ciocalteu method. The minimum inhibitory concentration (MIC) values of the extracts on Escherichia coli and Pseudomonas aeruginosa which are gram-negative pathogens, Staphylococcus aureus which is a gram-positive pathogen, and Candida albicans which is a fungus were measured by microdilution method. The MIC test range for propolis and perga was determined as 0.06µg/mL-125µg/mL, depending on the amount of phenolic compounds.Propolis showed an inhibitory effect at low concentrations against Candida albicans and Staphylococcus aureus compared to Escherichia coli and Pseudomonas aeruginosa. It has been determined that perga has an inhibitory effect at low concentrations against Escherichia coli, Pseudomonas aeruginosa and Candida albicans; although, it was determined that more higher concentrations are required for perga to show inhibitory activity against Staphylococcus aureus.As a result, it was observed that propolis and perga exhibited antimicrobial effects in our study.

Keywords

Anti-infective agents, Apitherapy, Microbial sensitivity tests, Propolis

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