BÖBREK İSKEMİ-REPERFÜZYON HASARINDA KALPAİN İNHİBİTÖRÜ OLAN AK295 UYGULANAN SIÇANLARDA SERUM ÜRE VE KREATİN DEĞERLERİ

Year 2022, Volume: 10 Issue: 1, 335 - 344, 10.03.2022

Songül Aydemir Mahmut Binen

https://doi.org/10.33715/inonusaglik.1021152

Abstract

Nekroz ve apoptoz gibi hücre ölümü ile sonuçlanan olaylarda kalpainler, kaspazlarla birlikte çalışan proteazlardır. Sıçan omurilik travma modellerinde kalpain inhibitörlerinin (AK295) apoptozu yavaşlattığı veya durdurduğu bilinmektedir. Bu çalışmada 28 adet erkek wistar albino sıçan rastgele seçilerek dört gruba ayrıldı. Gruplar; kontrol, İskemi-Reperfüzyon (İ/R), İskemi-Reperfüzyon+AK295, İskemi-Reperfüzyon+DMSO (dimetil sülfoksit) olarak belirlendi. İskemi sonrasında böbreklerde oluşan hasarda kalpain inhibitörü olan AK295’in etkisi incelendi. Sıçanlara sağ böbrek nefrektomisi uygulanarak 30 dakika total iskemi yapıldı. 24 saatlik reperfüzyon periyodu tamamlandıktan sonra anestezi uygulandı. Bu çalışmamızda, böbrek hasarında önemli indikatörler olan serum kreatin ve üre değerleri tayin edildi. Kontrol, I/R, I/R + AK295, and I/R + DMSO gruplarında ortalama üre değerleri; sırasıyla 35.4 ± 22.3, 156.4 ± 9.01, 150.8 ± 5.8, ve 165.2 ± 6.1 mg/dL olarak bulundu. İ/R + AK295 ve İ/R + DMSO değerlerinin, kontrol grubuna göre anlamlı (p<0.05) derecede yüksek olduğu bulunmuştur. Böbrekte İ/R oluşturulan sıçanlarda, AK295’in oluşan böbrek hasarını kısmen azalttığı tespit edildi.

Keywords

AK295, Böbrek, İskemi-Reperfüzyon, Kalpain inhibitörü

Supporting Institution

İnönü Üniversitesi

Project Number

2009/48

Thanks

Bu projeyi destekleyen İnönü Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi'ne teşekkür ediyorum.

Serum Urea and Creatin Values in Rats Administered with AK295, a Calpain Inhibitor, in Renal Ischemia-Reperfusion Injury

Abstract

In events that result in cell death such as necrosis and apoptosis, calpains are proteases functioning with caspases. Calpain inhibitors (AK295) are known to slow down or stop apoptosis in spinal cord trauma models in rats. In this study, 28 male Wistar albino rats were randomly selected and divided into four groups. Groups were determined as; control, Ischemia-Reperfusion (I/R), Ischemia-Reperfusion+AK295, Ischemia-Reperfusion+DMSO (dimethyl sulfoxide). On kidney damage after ischemia, the effect of AK295, a calpain inhibitor, was investigated. 30 minutes total ischemia was performed following right kidney nephrectomy application to rats. Anesthesia was administered after the reperfusion for 24-hour was completed. The serum creatinine and urea values, which are important indicators in kidney damage, were measured. The mean urea values in the groups of control, I/R, I/R + AK295, and I/R + DMSO were measured as 35.4 ± 22.3, 156.4 ± 9.01, 150.8 ± 5.8, and 165.2 ± 6.1 mg/dL, respectively. It has been determined that the results of groups I/R+AK295 and I/R+DMSO were statistically significantly higher than those of the control group (p<0.05). It was determined that the AK295 partly reduced kidney injury in rats, which have been performed renal IR.

Keywords

AK295, Calpain inhibitor, Ischemia-Reperfusion, Kidney

Project Number

2009/48

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