Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin

Volume: 6 Number: 3 November 1, 2012
  • M. M. Abu Teır
  • J. Ghıthan
  • S. Darwısh
  • M. M. Abu-hadıd
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Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin

Abstract

The interaction between cholesterol and human serum albumin has been investigated. The basic binding interaction was studied by UVabsorption and fluorescence spectroscopy. From spectral analysis cholesterol showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching mechanism. The binding constant (k) is estimated to be K=0.214 × 104 M-1 at 293 K. FTIR spectroscopy with Fourier self-deconvolution technique was used to determine the protein secondary structure and cholesterol binding mechanisms. The observed spectral changes indicate a higher percentage of H-bonding between cholesterol and a-helix (secondary structure motif in HSA) compared to the percentage of H-bonding between cholesterol and b-sheets (secondary structure motif in HSA)

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Primary Language

English

Subjects

-

Journal Section

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Authors

M. M. Abu Teır This is me

J. Ghıthan This is me

S. Darwısh This is me

M. M. Abu-hadıd This is me

Publication Date

November 1, 2012

Submission Date

November 1, 2012

Acceptance Date

-

Published in Issue

Year 2012 Volume: 6 Number: 3

IZ

https://izlik.org/JA54DS85DL

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RIS / Bibtex
APA
Teır, M. M. A., Ghıthan, J., Darwısh, S., & Abu-hadıd, M. M. (2012). Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. Journal of Applied Biological Sciences, 6(3), 45-55. https://izlik.org/JA54DS85DL
AMA
1.Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM. Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. J.appl.biol.sci. 2012;6(3):45-55. https://izlik.org/JA54DS85DL
Chicago
Teır, M. M. Abu, J. Ghıthan, S. Darwısh, and M. M. Abu-hadıd. 2012. “Multi-Spectroscopic Investigation of the Interactions Between Cholesterol and Human Serum Albumin”. Journal of Applied Biological Sciences 6 (3): 45-55. https://izlik.org/JA54DS85DL.
EndNote
Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM (November 1, 2012) Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. Journal of Applied Biological Sciences 6 3 45–55.
IEEE
[1]M. M. A. Teır, J. Ghıthan, S. Darwısh, and M. M. Abu-hadıd, “Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin”, J.appl.biol.sci., vol. 6, no. 3, pp. 45–55, Nov. 2012, [Online]. Available: https://izlik.org/JA54DS85DL
ISNAD
Teır, M. M. Abu - Ghıthan, J. - Darwısh, S. - Abu-hadıd, M. M. “Multi-Spectroscopic Investigation of the Interactions Between Cholesterol and Human Serum Albumin”. Journal of Applied Biological Sciences 6/3 (November 1, 2012): 45-55. https://izlik.org/JA54DS85DL.
JAMA
1.Teır MMA, Ghıthan J, Darwısh S, Abu-hadıd MM. Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. J.appl.biol.sci. 2012;6:45–55.
MLA
Teır, M. M. Abu, et al. “Multi-Spectroscopic Investigation of the Interactions Between Cholesterol and Human Serum Albumin”. Journal of Applied Biological Sciences, vol. 6, no. 3, Nov. 2012, pp. 45-55, https://izlik.org/JA54DS85DL.
Vancouver
1.M. M. Abu Teır, J. Ghıthan, S. Darwısh, M. M. Abu-hadıd. Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin. J.appl.biol.sci. [Internet]. 2012 Nov. 1;6(3):45-5. Available from: https://izlik.org/JA54DS85DL