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In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata) Liver Glucose 6-Phosphate Dehydrogenase

Year 2016, Volume: 10 Issue: 2, 46 - 50, 01.07.2016

Mahinur Kırıcı Muammer Kırıcı Şükrü Beydemir Metin Bülbül

Abstract

In this study, effects of some insecticides (cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin) on glucose 6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ oksidoreductase, EC 1.1.1.49; G6PD) from gilthead sea bream (Sparus aurata) have been investigated in vitro. In this purpose, G6PD enzyme was purified 1864.3-fold with a yield of 54.6% from fish liver using 2’,5’-ADP Sepharose 4B affinity chromatography. Activity of the enzyme was determined by Beutler method spectrophotometrically. The purify of the enzyme was checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and the purified enzyme showed a single band on the gel. Additionally, inhibitory effects of some insecticides (cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin) on G6PD were investigated. For this aim, IC50 values for cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin were determined by plotting activity % vs. [I]. IC50 values of cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin were 0.237, 0.016, 0.071, 0.021 and 0.187 mM, respectively. From these results, we showed that deltamethrin is the most potent inhibitor of fish liver G6PD enzyme

Keywords

Glucose 6-phosphate dehydrogenase Gilthead sea bream Insecticides Inhibition

Year 2016, Volume: 10 Issue: 2, 46 - 50, 01.07.2016

Mahinur Kırıcı Muammer Kırıcı Şükrü Beydemir Metin Bülbül

Abstract

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Details

Other ID JA42JD53HM
Journal Section Research Article
Authors

Mahinur Kırıcı This is me

Muammer Kırıcı This is me

Şükrü Beydemir This is me

Metin Bülbül This is me

Publication Date July 1, 2016
Published in Issue Year 2016 Volume: 10 Issue: 2

Cite

APA Kırıcı, M., Kırıcı, M., Beydemir, Ş., Bülbül, M. (2016). In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata) Liver Glucose 6-Phosphate Dehydrogenase. Journal of Applied Biological Sciences, 10(2), 46-50.
AMA Kırıcı M, Kırıcı M, Beydemir Ş, Bülbül M. In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata) Liver Glucose 6-Phosphate Dehydrogenase. J.appl.biol.sci. July 2016;10(2):46-50.
Chicago Kırıcı, Mahinur, Muammer Kırıcı, Şükrü Beydemir, and Metin Bülbül. “In Vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus Aurata) Liver Glucose 6-Phosphate Dehydrogenase”. Journal of Applied Biological Sciences 10, no. 2 (July 2016): 46-50.
EndNote Kırıcı M, Kırıcı M, Beydemir Ş, Bülbül M (July 1, 2016) In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata) Liver Glucose 6-Phosphate Dehydrogenase. Journal of Applied Biological Sciences 10 2 46–50.
IEEE M. Kırıcı, M. Kırıcı, Ş. Beydemir, and M. Bülbül, “In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata) Liver Glucose 6-Phosphate Dehydrogenase”, J.appl.biol.sci., vol. 10, no. 2, pp. 46–50, 2016.
ISNAD Kırıcı, Mahinur et al. “In Vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus Aurata) Liver Glucose 6-Phosphate Dehydrogenase”. Journal of Applied Biological Sciences 10/2 (July 2016), 46-50.
JAMA Kırıcı M, Kırıcı M, Beydemir Ş, Bülbül M. In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata) Liver Glucose 6-Phosphate Dehydrogenase. J.appl.biol.sci. 2016;10:46–50.
MLA Kırıcı, Mahinur et al. “In Vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus Aurata) Liver Glucose 6-Phosphate Dehydrogenase”. Journal of Applied Biological Sciences, vol. 10, no. 2, 2016, pp. 46-50.
Vancouver Kırıcı M, Kırıcı M, Beydemir Ş, Bülbül M. In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata) Liver Glucose 6-Phosphate Dehydrogenase. J.appl.biol.sci. 2016;10(2):46-50.