Insecticidal Effects of Some Bacillus thuringiensis Commercial Biopreparats on the Larvae of the Tomato Leaf Miner, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae)

Year 2022, , 632 - 636, 30.12.2022

Alime Bayındır Erol , Oktay Erdoğan

https://doi.org/10.31015/jaefs.2022.4.17

Abstract

Effects of 5 commercial biopreparats named; Dacron®, Florbac®, Dipel®, Delfin® and Rebound® containing different Bacillus thuringiensis (Bt) strains on the 3rd instar larvae of the tomato leaf miner, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) were determined under laboratory conditions. T. absoluta, tomato (Gusto F1) variety and some commercial biopreparats were used as materials in the study. Production of T. absoluta larvae were performed under the conditions of the growth chambers where the tomato variety was grown (25±1°C, 60±5% RH and 16:8 photoperiod). Under laboratory conditions, 1, 3 and 5% concentrations of commercial biopreparats were applied on the 3rd instar larvae of the pest, and the experiment was carried out with 5 replications according to a randomized parcels design. As a result of the application of 1% IU/mg concentrations of Dacron® and Delfin® commercial biopreparats, 100% mortality rate was determined in the 3rd instar larvae of T. absoluta on the 7th day counts. When 3 and 5% IU/mg concentrations of all commercial biopreparats were applied, a mortality rate of 100% was recorded on the 3rd instar larvae of T. absoluta on the 5th and 7th day counts. In-vitro, as the concentration of commercial biopreparats increased, mortality rate of the 3rd instar larvae of T. absoluta increased. However, it would be appropriate to conduct field trials with the same commercial biopreparats to recommend their use of them commercially.

Keywords

Bacillus thuringiensis, Tuta absoluta, Mortality rate, Biopesticide

References

• Abbott, W.S. (1925). A method of computing the effectiveness of an insecticide. J Econ Entomol 18(2):265–267.
• Alsaedi, G., Ashouri, A., Talaei-Hassanloui, R. (2017). Evaluation of Bacillus thuringiensis to control Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) under laboratory conditions. Agric Sci 8: 591-599.
• Anonymous, (2016).Combating tomato diseases and pests. Ministry of Food, Agriculture and Livestock, 64p.
• Cabello, T., Gallego, J.R., Vila, E., Soler, A., del Pino, M., Carnero, A., Hernandez, S., Polaszek, A. (2009). Biological control of the South American tomato pinworm, Tuta absoluta (Lepidoptera: Gelechiidae), with releases of Trichogramma achaeae (Hymenoptera: Trichogrammatidae) on tomato greenhouse of Spain. IOBC-WPRS Bulletin, 49, 225-230.
• Catalbudak, E.S., Aydın, A,, Durmusoglu, E. (2018), Determination of efficacy of some biopreparats on Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) larvae. Turk Bull Entomol 8(4): 113-122.
• Duman, İ. (2016). Industrial tomato cultivation. Journal of Turkish Seed Association 17:18-21.
• Durmusoglu, E., Tiryaki, O., Canhilal, R. (2010). Pesticide use, residue and durability problems in Turkey. Turkey Agricultural Engineering 7th Technical Congress, Ankara, Proceedings 2, 589-607.
• Doganlar, M, Yıldırım, A.E., Yigit, A. (2015). Efficacy of Bacillus thuringiensis var. kurstaki and some environmentally friendly pesticides on the Tomato moth, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Turk J Biol Control, 6(1): 13-24. Feitelson, J.S., Payne, J., Kim, L. (1992). Bacillus thuringiensis: insects and beyond. BioTechnology 10: 271–275.
• Giustolin, T.A., Vendramim, J.D., Alves, S.B., Vieira, S.A., Pereira, R.M. (2001). Susceptibility of Tuta absoluta (Meyrick) (Lep., Gelechiidae) reared on two species of Lycopersicon to Bacillus thuringiensis var. kurstaki. J Appl Entomol 125: 551-556. Gonzalez-Cabrera, J., Mollà, O., Montan, H., Urbaneja, A. (2011). Efficacy of Bacillus thuringiensis (Berliner) in controlling the tomato borer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). BioControl 56: 71–80.
• Jallow, M.F.A., Dahab, A.A., Albaho, M.S., Devi, V.Y. (2018). Efficacy of some biorational insecticides against Tuta absoluta (Meyrick) (Lepidoptera Gelechiidae) under laboratory and greenhouse conditions in Kuwait. J. Appl. Entomol 143: 187–195.
• Karabörklü, S., Azizoğlu, U., Azizoglu, Z.B. (2018). Recombinant entomopathogenic agents: a review of biotechnological approaches to pest insect control. W J Microbiol Biotech, 34 (1): 14.
• Kılıc, T. (2010). First record of Tuta absoluta in Turkey. Phytoparasitica, 38 (3): 243-244.
• Moussa, S., Baiomy, F., Sharma, A., El-Adl, F.E. (2013). The status of tomato leafminer Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in Egypt and potential effective pesticides. Academic J Entomol 6(3): 110-115.
• Pazos, L., Salamanca, C. (2007). Three-dimensional structure of Bacillus thuringiensis toxins. Acta Biol Colomb 12: 19-32.
• Rezaei, A.A., Talaei-Hassanlous, R. (2016). The use of Bacillus thuringiensis-based products in biocontrol of tomato leaf miner, Tuta absoluta (Lepidoptera Gelechiidae). Int. J. Agric. Innov. Res. 4: 814–817.
• Siqueira, H.A., Guedes, R.N., Picanco, M.C. (2000). Insecticide resistance in populations of Tuta absoluta (Lepidoptera: Gelechiidae). Agricultural and Forest Entomology, 2, 147-153.
• Satıs, Ü. (2013). Effect of Bacillus thuringiensis var. kurstaki and Chlorantraniliprole+Abamectin on larve of the tomato leafminer Tuta absoluta (Meyrick, 1917) (Lepidoptera:Gelechiidae) on tomato (Lycopersicon esculentum Mill.). Harran University Institute of Science and Technology, Master Thesis, 46 p.
• Tsoulnara, D., Port, G. (2016). Efficacy of a Beauveria bassiana strain, Bacillus thuringiensis and their combination against the tomato leafminer Tuta absoluta. Entomol Hellenica 25: 23-30. Youssef, N.A., Hassan, G.M. (2013). Bioinsecticide activity of Bacillus thuringiensis isolates on tomato borer, Tuta absoluta (Meyrick) and their molecular identification. African J Biotech 12: 3699-3709.
• Zappala, L,, Biondi, A., Alma, A., Al-Jboory, I.J., Arno, J., Bayram, A., Chailleux, A., El-Arnaouty, A., Gerling, D., Guenaoui, Y., Shaltiel-Harpaz, L., Siscaro, G., Stavrinides, M., Tavella, L., Aznar., R.V., Urbaneja, A., Desneux N. (2013) Natural enemies of the South American moth, Tuta absoluta, in Europe, North Africa and Middle East, and their potential use in pest control strategies. J Pest Sci 86:635–647.