Rhizoctonia species, anastomosis groups, and pathogenicity isolated from common bean in Lake Van Basin, Turkiye

Year 2024, , 359 - 368, 27.06.2024

Emre Demirer Durak , Çeknas Erdinç , Aytekin Ekincialp

https://doi.org/10.31015/jaefs.2024.2.11

Abstract

Common bean (Phaseolus vulgaris L.) is an important vegetable crop grown in Lake Van Basin. Local genotypes are widely grown in the region. Rhizoctonia root rot induced by Rhizoctonia solani Kühn is an important soilborne plant disease that leads to global economic losses as well as in Turkey. The present study was conducted to determine anastomosis groups and pathogenicity of Rhizoctonia spp. obtained from bean plants in Lake Van Basin in 2013 and 2014. A total of 236 Rhizoctonia isolates in 5 anastomosis groups were obtained from bean plant roots. It was observed that AG- 4 (112) was the most isolated group in beans, followed by AG- 2 (41), AG- 3 (28), AG- 5 (33), and binucleate AG- K (22) isolates. Pathogenicity test conducted in thirty isolates in 5 anastomosis groups was analyzed for A64 (Bitlis/ Adilcevaz), TR68557 genotypes, and Gina (cv.) under growth chamber conditions. The study findings demonstrated that all tested isolates could infect the bean plant with different degrees of severity; however, the most virulent group was AG- 4. It was determined that the most virulent isolate was Isolate No. 19 in the A64 genotype, Isolate No. 2 in TR68557, and Isolate No. 18 in Gina cv. in in vivo tests. The identification and pathogenicity determination of Rhizoctonia isolates are the first steps towards an efficient control strategy for bean diseases caused by Rhizoctonia species. In order to obtain quality and productive products in the Van Lake Basin, where intensive bean production is carried out, precautions should be taken by considering the damage caused by Rhizoctonia spp. on plants.

Keywords

Anastomosis group, Bean, Pathogenicity, Rhizoctonia spp.

Supporting Institution

The author would like to thank the Scientific Research Project (BAP) Commission of Van Yuzuncu Yil University for funding the study (Project no 2013- ZF- B033).

Project Number

2013- ZF- B033

References

• Akarca, Z., Demirci, E. (2022). Erzincan İlinde Fasulye Baklalarından İzole Edilen Rhizoctonia Türlerinin Anastomosis Grupları ve Patojenisitesi. The Journal of Turkish Phytopathology, 51, 39-48.
• Aydın, M.H., Ünal, F. (2021). Anastomosis Groups and Pathogenicity of Rhizoctonia solani Kühn Isolates Obtained from Pistachio (Pistacia vera L.) Saplings in Siirt Province, Turkey. Türkiye Tarımsal Araştırmalar Dergisi, 8(1), 18-26. doi: 10.19159/tutad.781041
• Bolkan, H.A., Ribeiro, W.R.C. (1985). Anastomosis groups and pathogenicity of Rhizoctonia solani isolates from Brazil. Plant Disease, 69, 599-601. https://doi.org/10.1094/pd-69-599
• Cankara, B. (2019). Determination of the reactions of some bean varieties to Rhizoctonia solani. Ankara University Graduate School of Natural and Applied Sciences Department of Plant Protection. Ankara, 40 p.
• Canpolat, S., Woodward, S., Kurbetli, İ. (2023). Molecular and pathological characterization of the isolates of Rhizoctonia spp. associated with dry bean (Phaseolus vulgaris) in Türkiye. Journal of Plant Pathology, 105(3), 837-848. doi.org/10.1007/s42161-023-01377-2
• Conner, R.L., Hou, A., Balasubramanian, P., McLaren, D.L., Henriquez, M.A., Chang, K.F., McRae, K.B. (2014). Reaction of dry bean cultivars grown in western Canada to root rot inoculation. Canadian Journal of Plant Science, 94, 1219-1230. doi:10.4141/CJPS2013-416
• Costa-Coelho, G.R., Café Filho, A.C., Lobo Jr, M. (2014). A comparison of web blight epidemics on common bean cultivars with different growth habits. Crop Protection, 55, 16-20. doi.org/10.1016/j.cropro.2013.10.006
• Demirci, E., Döken, M.T. (1992). Interactions between various cultivated plants and the anastomosis groups of Rhizoctonia solani Kuhn isolated from potatoes in Erzurum region. Kükem, 15, 33-38.
• Demirci, E., Döken, M.T. (1993). Anastomosis groups and pathogenicity of Rhizoctonia solani Kühn isolates from potatoes in Erzurum, Türkiye. Journal of Turkish Phytopathology, 22, 95-102.
• Demirci, E., Döken, M.T. (1995). Anastomosis groups of Rhizoctonia solani Kühn and binucleate Rhizoctonia isolates from various crops in Türkiye. Journal of Turkish Phytopathology, 24, 57-62.
• Dong, W., Li, Y., Duan, C., Li, X., Naito, S., Conner, R.L., Yang, G., Li, C. (2017). Identification of AG V, a new anastomosis group of binucleate Rhizoctonia spp. from taro and ginger in Yunnan province. European Journal of Plant Pathology, 148, 839- 851. doi:10.1007/s10658-016-1144-y
• Eken, C., Demirci, E. (2004). Anastomosis groups and pathogenicity of Rhizoctonia solani and Binücleate Rhizoctonia isolates from bean in Erzurum, Turkey. Journal of Plant Pathology, 86, 49-52.
• Ekincialp, A., Şensoy, S. (2018). Phenotypic and molecular determination of anthracnose disease resistance in Lake Van Basin's bean genotypes (Phaseolus vulgaris L.). Legume Research-An International Journal, 41, 135-142. doi:10.18805/lr.v0i0.7858
• Erper, İ. (2003). Anastomosis Groups, Characteristic Traits and Pathogenicities of Rhizoctonia Group Fungi Determined on Some Legume Species in Samsun Province. University of Ondokuz Mayıs, Samsun, Turkey.
• Erper, İ., Özkoç, İ., Karaca, G.H. (2011). Identification and pathogenicity of Rhizoctonia species isolated from bean and soybean plants in Samsun, Turkey. Archives of Phytopathology and Plant Protection, 44, 78–84. doi: https://doi.org/10.1080/03235400903395427
• Faostat, (2017). Food and Agriculture Organization of the United Nations. http://www.fao.org/faostat/en/ Erişim tarihi: 11.07.2017.
• Gavilanes, F.Z., Andrade, D.S., Figueiredo, A., Cedeño-García, G., Zucareli, C., Filho, J.T., Guimarães, M.F. (2020). Effect of physic nut seed cake on common bean development and clay dispersion of soil. Polish Journal of Environmental Studies, 29, 2177-2184. doi: https://doi.org/10.15244/pjoes/109726
• Genç Kesimci, T., Eken, C., Demirci, E. (2022). Bakla Bitkilerinde Kök Çürüklüğüne Neden Olan Rhizoctonia Türlerinin Anastomosis Grupları ve Patojeniteleri. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 12(2): 550-562. https://doi.org/10.21597/jist.1067642
• Gossen, B.D., Conner, R.L., Chang, K.F., Pasche, J.S., McLaren, D.L., Henriquez, M.A., Hwang, S.F. (2016). Identifying and managing root rot of pulses on the northern great plains. Plant Disease, 100, 1965-1978. doi:https://doi.org/10.1094/PDIS-02-16-0184-FE
• Jaiswal, A.K., Elad, Y., Graber, E.R., Frenkel, O. (2014). Rhizoctonia solani suppression and plant growth promotion in cucumber as affected by biochar pyrolysis temperature, feedstock and concentration. Soil Biology & Biochemistry, 69, 110-118. https://doi.org/10.1016/j.soilbio.2013.10.051
• Karaca, G.H., Özkoç, I., Erper, I. (2002). Determination of the anastomosis grouping and virulence of Rhizoctonia solani Kühn isolates associated with bean plants grown in Samsun/Turkey. Pakistan Journal of Biological Sciences 5, 434-437. https://doi.org/10.3923/pjbs.2002.434.437
• Kılıçoğlu, M.Ç., Özkoç, İ. (2013). Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on ITS-5.8 S rDNA. Turkish Journal of Biology, 37,18-24. doi:10.3906/biy-1202-17
• Marcou, S., Wikström, M., Ragnarsson, S., Persson, L., Höfte, M. (2021). Occurrence and Anastomosis Grouping of Rhizoctonia spp. Inducing Black Scurf and Greyish-White Felt-Like Mycelium on Carrot in Sweden. J. Fungi, 7, 396. doi: https://doi.org/10.3390/jof7050396
• Matloob, A.H., Juber, K.S. (2013). Biological control of bean root rot disease caused by Rhizoctonia solani under green house and field conditions. Agriculture and Biology Journal of North America, 4, 512-519.
• Misawa, T., Kurose, D. (2019). Anastomosis group and subgroup identification of Rhizoctonia solani strains deposited in NARO Genebank, Japan. J Gen Plant Pathol 85:282–294, https://doi.org/10.1007/s10327-019-00848-8
• Mora-Umaña, F., Barboza, N., Alvarado, R., Vásquez, M., Godoy-Lutz, G., Steadman, J.R., Ramírez, P. (2013). Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean. Tropical Plant Pathology, 38, 461-471. https://doi.org/10.1590/s1982-56762013000600001
• Muyolo, N.G., Lipps, P.E., Schmıtthenner, A.F. (1993). Reactions of dry bean, lima bean, and soybean cultivars to Rhizoctonia root and hypocotyl rot and web blight. Plant Disease, 77, 234-238. https://doi.org/10.1094/pd-77-0234
• Naik, T.S., Somasekhara, Y.M., Raja, Amaresh, Y.S., Zaheer Ahamed. (2016). In-vitro evaluation of fungal antagonists and plant extracts against French bean root rot caused by Rhizoctonia solani Khün. Plant Archives, 16, 159-163.
• Nandeesha, C.V., Nandeesha, K.L., Bhaliya, C.M. (2021). Evolutionary biology and interaction among the anastomosis groups of Rhizoctonia spp. Journal of Pharmacognosy and Phytochemistry, 10, 461-466. doi: https://doi.org/10.22271/phyto.2021.v10.i2f.13840
• Nerey, Y., Pannecoucque, J., Hernandez, H.P., Diaz, M., Espinosa, R., Stephanie, D.V., Sarah, V.B., Herrera, L., Höfte, M. (2010). Rhizoctonia spp. causing root and hypocotyl rot in Phaseolus vulgaris in Cuba. Journal of Phytopathology, 158, 236-243. doi:10.1111/j.1439-0434.2009.01609.x
• Ntatsi, G., Gutiérrez-Cortines, M., Karapanos, I., Barros, A., Weiss, J., Balliu, A. (2018). The quality of leguminous vegetables as influenced by preharvest factors. Sci. Hortic. 232, 191-205. doi: https://doi.org/10.1016/j.scienta.2017.12.058
• Ogoshi, A. (1975). Grouping of Rhizoctonia solani Kühn and their perfect stages. Review Plant Protection Research, 8, 93-103.
• Oladzad, A., Zitnick-Anderson, K., Jain, S., Simons, K., Osorno, J.M., McClean, P.E., Pasche, J.S. (2019). Genotypes and genomic regions associated with Rhizoctonia solani resistance in common bean. Front. Plant Science, 10, 956. doi: https://doi.org/10.3389/fpls.2019.00956
• Omar, A.I., Erkılıç, A., Mohammed, M.A. (2021). Determination of fungal pathogens causing root-rot on bean plants in bean production areas of Nevşehir province, Turkey. The Journal of Turkish Phytopathology, 50, 9-16.
• Palacıoglu, G., Cankara, B., Bayraktar, H., Ozer, G. (2019). Efficacy of seed treatments with some fungicides against bean root rot caused by Rhizoctonia solani. International Journal of Agriculture and Wildlife Science, 5, 96 – 102. https://doi.org/10.24180/ijaws.533240
• Palacıoğlu, G., Cankara, B., Bayraktar, H., Özer, G. (2024). Genetic and pathogenic characterization of Rhizoctonia solani AG-4 isolates obtained from common bean. Physiological and Molecular Plant Pathology, 131, 102277. DOI:10.1016/j.pmpp.2024.102277
• Papavizas, G.C., Adams, P.B., Lumsden, R.D., Lewis, J.A., Dow, R. L., Ayers, W.A., Kantzes, J.G. (1975). Ecology and Epidemiology of Rhizoctonia solani in field soil. Phytopathology, 65, 871–877. https://doi.org/10.1094/phyto-65-871
• Parmeter, J.R., Sherwood, J.R., Platt, W.D. (1969). Anastomosis grouping among isolates of Thanatephorus cucumeris. Phytopathology, 5, 1270-1278.
• Rovira A.D., Ogoshi A., Mcdonald H.J. (1986). Characterization of isolates of Rhizoctonia solani from cereal roots in South Australia and New South Wales. Phytopathology, 76, 1245-1248. https://doi.org/10.1094/phyto-76-1245
• Sharon, M., Kuninaga, S., Hyakumachi, M., Naito, S., Sneh, B. (2008). Classification of Rhizoctonia spp. using rDNA-ITS sequence analysis supports the genetic basis of the classical anastomosis grouping. Mycoscience, 49, 93-114. doi: 10.1007/s10267-007-0394-0
• Sneh, B., Burpee, L., Ogoshi, A. (1991). Identification of Rhizoctonia species. St. Paul, MN: APS Press. p. 133.
• Spedaletti, Y., Aparicio, M., Mercado Cárdenas, G., Rodriguero, M., Taboada, G., Aban, C., Galván, M. (2016). Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina. Journal of Phytopathology, 164, 1054-1063. doi: https://doi.org/10.1111/jph.12526
• Suárez-Martínez, S.E., Ferriz-Martínez, R.A., Campos-Vega, R., Elton-Puente, J.E., Carbot, K.T., García-Gasca, T. (2016). Bean seeds: leading nutraceutical source for human health. J. Food, 14, 131–137. doi:https://doi.org/10.1080/19476337.2015.1063548
• Temizel, M.M., Ertunç, F. (1992). Investigations on the detection of bean diseases of Van province. Journal of Turkish Phytopathology, 21, 25-31.
• Tuncer, G., Erdiller, G. (1990). The identification of Rhizoctonia solani Kühn anastomosis groups isolated from potato and some other crops in Central Anatolia. Journal of Turkish Phytopathology, 19, 89-93.
• Valentín Torres, S., Vargas, M.M., Godoy-Lutz, G., Porch, T.G., Beaver, J.S. (2016). Isolates of Rhizoctonia solani can produce both web blight and root rot symptoms in common bean (Phaseolus vulgaris L.). Plant Disease, 100, 1351-1357. doi:https://doi.org/10.1094/PDIS-11-15-1270-RE
• Yang, Y.G., Zhao, C., Guo, Z.J., Wu, X.H. (2015). Characterization of a new anastomosis group (AG W) of binucleate Rhizoctonia, causal agent for potato stem canker. Plant Diseases, 99, 1757- 1763. doi: https://doi.org/10.1094/PDIS-01-15-0036-RE
• Yıldırım, E., Erper, I. (2017). Characterization and pathogenicity of Rhizoctonia spp. isolated from vegetable crops grown in greenhouses in Samsun province, Turkey. Bioscience Journal, 33, 257-267. https://doi.org/10.14393/bj-v33n2-34580