Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L.

Murat Güler; Hatice Öğütcü

doi:10.31015/jaefs.2024.2.16

EN

Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L.

Abstract

Plant growth-promoting rhizobacteria (PGPR) support plant growth through direct and indirect mechanisms. To investigate PGPR strains that support plant growth, 21 bacterial isolates, mostly Bacillus ssp. and Pseudomonas ssp., were isolated from different rhizospheric soils of sunflowers in Kırşehir districts in 2020. All isolates were characterized morphologically, biochemically by screening under in vitro conditions for plant growth-promoting properties such as nitrogen fixation, IAA (indoleacetic acid) production, siderophore production, HCN (hydrogen cyanide) production, inorganic phosphate solubility. It was also screened for extracellular enzyme production and antifungal activity against Fusarium oxysporum. Among the 21 isolates, 3 isolates (MH-35-4, MH-49-4, MH-64-3) fixed nitrogen, 2 isolates (MH-59-6, MH-64-3), produced siderophores, 8 isolates (MH-35-4, MH-35-6, MH-54-3, MH-54-4, MH-59-1, MH-59-2, MH-59-4, MH-59-8) produced HCN, 6 isolates (MH-35-6, MH-54-4, MH-59-1, MH-59-2, MH-59-4, MH-59-8) produced IAA, and 7 isolates (MH-35-4, MH-35-6, MH-59-1, MH-59-2, MH-59-4, MH-59-8, MH-64-3) solubilized inorganic phosphate. Additionally, only 2 isolates (MH-54-3, MH-54-4) were positive amylase tests, 8 isolates (MH-35-6, MH-54-4, MH-59-1, MH-59-2, MH-59-4, MH-59-6, MH-59-7, MH-59-8) were positive citrate tests, 8 isolates (MH-35-1, MH-35-4, MH-35-7, MH-49-4, MH-54-4, MH-59-6, MH-59-7, MH-64-3) were positive protease tests, and 6 isolates (MH-35-1, MH-35-3, MH-35-7, MH-54-3, MH-54-4, MH-59-7) were positive gelatin hydrolysis tests. Among 21 isolates, 38% were determined as hydrogen cyanide producers, 10% as siderophore producers, 29% IAA producers, 33% as phosphate solubilizers and 14% as nitrogen fixers. Isolate MH-35-6 showed the highest antifungal activity against Fusarium oxysporum with an inhibition rate of 53.57%. This was followed by isolates MH-54-1 (51.19%), MH-54-3 (47.61%) and MH-59-2 (38.09%), respectively. Therefore, our study reveals that bacteria that promote plant growth in sunflowers can be used to increase crop yield and as a biocontrol agent.

Keywords

Helianthus annuus L., Plant growth-promoting rhizobacteria (PGPR), Fusarium oxysporum, MALDI-TOF MS

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Details

Primary Language

English

Subjects

Organic Agriculture, Soil Biology, Soil Microbiology

Journal Section

Research Article

Authors

Murat Güler ^*
0000-0002-3074-6458
Türkiye

Hatice Öğütcü
0000-0001-7100-9318
Türkiye

Early Pub Date

June 24, 2024

Publication Date

June 27, 2024

Submission Date

March 26, 2024

Acceptance Date

June 11, 2024

Published in Issue

Year 2024 Volume: 8 Number: 2

DOI

https://doi.org/10.31015/jaefs.2024.2.16

IZ

https://izlik.org/JA25FS55YB

APA

Güler, M., & Öğütcü, H. (2024). Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L. International Journal of Agriculture Environment and Food Sciences, 8(2), 412-429. https://doi.org/10.31015/jaefs.2024.2.16

AMA

1.Güler M, Öğütcü H. Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L. int. j. agric. environ. food sci. 2024;8(2):412-429. doi:10.31015/jaefs.2024.2.16

Chicago

Güler, Murat, and Hatice Öğütcü. 2024. “Isolation and Characterization of Plant Growth Promoting Rhizobacteria (PGPR) from Rhizosphere of Helianthus Annuus L”. International Journal of Agriculture Environment and Food Sciences 8 (2): 412-29. https://doi.org/10.31015/jaefs.2024.2.16.

EndNote

Güler M, Öğütcü H (June 1, 2024) Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L. International Journal of Agriculture Environment and Food Sciences 8 2 412–429.

IEEE

[1]M. Güler and H. Öğütcü, “Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L”., int. j. agric. environ. food sci., vol. 8, no. 2, pp. 412–429, June 2024, doi: 10.31015/jaefs.2024.2.16.

ISNAD

Güler, Murat - Öğütcü, Hatice. “Isolation and Characterization of Plant Growth Promoting Rhizobacteria (PGPR) from Rhizosphere of Helianthus Annuus L”. International Journal of Agriculture Environment and Food Sciences 8/2 (June 1, 2024): 412-429. https://doi.org/10.31015/jaefs.2024.2.16.

JAMA

1.Güler M, Öğütcü H. Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L. int. j. agric. environ. food sci. 2024;8:412–429.

MLA

Güler, Murat, and Hatice Öğütcü. “Isolation and Characterization of Plant Growth Promoting Rhizobacteria (PGPR) from Rhizosphere of Helianthus Annuus L”. International Journal of Agriculture Environment and Food Sciences, vol. 8, no. 2, June 2024, pp. 412-29, doi:10.31015/jaefs.2024.2.16.

Vancouver

1.Murat Güler, Hatice Öğütcü. Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L. int. j. agric. environ. food sci. 2024 Jun. 1;8(2):412-29. doi:10.31015/jaefs.2024.2.16

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Abstract

Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L.

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Cited By

Evolution of plant growth promoting rhizobacteria under global climate change

Bacterial allies in chromium hyperaccumulation: native rhizobacterial dynamics of profusely growing Dactyloctenium aegyptium in highly tainted tannery sludge

Endophytes and Plants Interaction: A Hidden Microbial World Inside the Plant

Isolation and Screening of Maize Growth-Promoting Rhizobacteria for the Production of Biofertilizer: A Case Study of the Hauts Bassins in Burkina Faso

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