Nematicidal effect of powder extractions of different coloured radish seeds against Meloidogyne incognita on tomato

Year 2024, , 674 - 680, 29.09.2024

Fatma Gül Göze Özdemir , Fadimana Maril , Harun Çimenkaya , Bekir Tosun

https://doi.org/10.31015/jaefs.2024.3.21

Abstract

The study aimed to evaluate the nematicidal effect of powder extracts of different coloured radish seeds against Meloidogyne incognita on tomato (Gülizar F1, susceptibile to root-knot nematode) under controlled conditions. Extractions were obtained from radish seeds of different colours (white, black, red, yellow) by using ethanol and acetone solvents. The experiment was carried out using 2, 4 and 6 g powder/plant application of the extracts in the pot. The experiment was set up in a random plot design with 5 replication for each radish seed extract and concentration. Radish seed powder was applied one week after nematode inoculation (1000 M. incognita eggs). After 50 days, the number of galls and egg masses on the roots were counted. It was determined that radish colour, extraction solvents and concentrations of extracts differed significantly for their nematicidal effects. The mean number of galls and egg masses was found to be 56 units in the negative control. Compared to the negative control, all treatments and concentrations decreased the number of galls and egg masses. The number of galls and egg masses was lower in acetone extract than in ethanole extract. The nematicidal effect was higher in yellow and red radish seeds powder application. The highest nematicidal effect was determined at 6 g powder/plant application. While the mean number of galls was 1.4 unit in the yellow seed powder application at a concentration of 6 g/plant of the extract prepared with acetone solvent, it was found to be 3.0 units in the red seed powder application at 6 g/plant of the acetone extract. The number of egg masses was 1.0 unit in the yellow seed powder application, while it was 2.8 units in the red seed powder application at 6 g/plant of the acetone extract. The acetone extract of radish seed powder can be used as an alternative to chemicals in the root-knot nematodes control.

Keywords

Radish, Seed powder, acetone extract, Ethanol extract, Nematicidal effect, Root-knot nematode

Supporting Institution

It was supported by the TÜBİTAK 2209-A University Students Research Projects Support Program with the project number 1919B012200676 in the 2022-1 period.

Project Number

1919B012200676

References

• Ahmad, F., Hasan, I., Chishti, D. K., & Ahmad, H. (2012). Antibacterial activity of Raphanus sativus Linn. seed extract. Global Journal of Medical Research, 12(11), 25-34.
• Aissani, N., & Sebai, H. (2022). Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita. Journal of Nematology, 54(1). doi: 10.2478/jofnem-2022-0050.
• Aji, M. B. (2024). Use of Plant Material in the Management of Plant Parasitic Nematodes. In Nematodes-Ecology, Adaptation and Parasitism. IntechOpen. doı: 10.5772/intechopen.1002742
• Aydınlı, G., & Mennan, S. (2018). Biofumigation studies by using Raphanus sativus and Eruca sativa as a winter cycle crops to control root-knot nematodes. Brazilian Archives of Biology and Technology, 61. DOI: 10.1590/1678-4324-2018180249.
• Bawa, J. A., Mohammed, I., & Lıadı, S. (2014). Nematicidal effect of some plants extracts on root-knot nematodes (Meloidogyne incognita) of tomato (Lycopersicon esculentum). World Journal of Life Sciences and Medical Research, 3(3), 81.
• El-Abbassi, A., Kiai, H., & Hafidi, A. (2012). Phenolic profile and antioxidant activities of olive mill wastewater. Food chemistry, 132(1), 406-412. doi: 10.1016/j.foodchem.2011.11.013
• El-Shaefeey, E. I., El-Sheikh, M. A., El-Khatieb, A. A., & Heikal, H. M. (2023). Effect of Certain Soil Amendments on Root-Knot Nematode, Meloidogyne javanica, Affecting Eggplant. Journal of Plant Protection and Pathology, 14(1), 41-48. DOI: 10.21608/jppp.2023.166339.1101
• Fourie, H., Ahuja, P., Lammers, J., & Daneel, M. (2016). Brassicacea-based management strategies as an alternative to combat nematode pests: A synopsis. Crop Protection, 80, 21-41. https://doi.org/10.1016/j.cropro.2015.10.026
• Goverse, A., and Smant, G. (2014). The activation and suppression of plant innate immunity by parasitic nematodes. Annual Review Phytopathology, 52, 243–265. https://doi.org/10.1146/annurev-phyto-102313-050118
• Goyeneche, R., Roura, S., Ponce, A., Vega-Gálvez, A., Quispe-Fuentes, I., Uribe, E., & Di Scala, K. (2015). Chemical characterization and antioxidant capacity of red radish (Raphanus sativus L.) leaves and roots. Journal of functional foods, 16, 256-264. https://doi.org/10.1016/j.jff.2015.04.049
• Göze Özdemir, F. G. (2022). Suppressive effect of seed powders of some Brassicaceae plants on Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) in tomato and cucumber. Turkish Journal of Entomology, 46(4), 421-430. https://doi.org/10.16970/entoted.1127262
• Göze Özdemir, F. G. (2024). Effect of Silybum marianum (L.) Gaertn. Leaf and Seed Extracts Prepared Using Different Solvents on Root-Knot Nematode. Akademik Ziraat Dergisi, 13(1), 111-118. https://doi.org/10.29278/azd.1461164
• Hooper, D. J., Hallmann, J., & Subbotin, S. A. (2005). Methods for extraction, processing and detection of plant and soil nematodes. In Plant parasitic nematodes in subtropical and tropical agriculture (pp. 53-86). Wallingford UK: CABI Publishing. https://doi.org/10.1079/9780851997278.0053
• Hussey, R. S., & Barker, K. R. (1973). A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter, 57(12), 1025-1028.
• Ibrahim , I.K.A., El-Saedy, M.A.M., Mokbel, A.A. (2007). Control of the root-knot nematode Meloidogyne incognita on sunflower plants with certain organic plant materials and biocontrol agents. Egyptian Journal of Phytopathol , 35 ( 1 ) : 13 – 24 .
• Jaafar, N. A., Ahmed, A. S., & Al-Sandooq, D. L. (2020). Detection of active compounds in radish Raphanus Sativus L. and their various biological effects. Plant Arch, 20(2), 1647-50.
• Kabil, F. F., & Adam, M. (2020). Nematıcıdal Actıvıty of Garden Cress bıo actıve bioactive ıngredıents ingredients agaınst Root Knot Nematode (Meloıdogyne incognıta) Infected Tomato. Transplants Plant Archives, 20 (2), 9301-9310.
• Khatiwada, B., Kunwar, S., Dhakal, A., Joshi, A., Miya, A. R., & Subedi, P. (2018). Total phenolic content, antioxidant activity, alpha-amylase inhibitory activity and antibacterial activity of radish seed and rapeseed. European Journal of Biotechnology and Bioscience, 6, 21-25.
• Lazzeri, L., Curto, G., Dallavalle, E., D’avino, L., Malaguti, L., Santi, R., & Patalano, G. (2009). Nematicidal efficacy of biofumigation by defatted Brassicaceae meal for control of Meloidogyne incognita (Kofoid et White) Chitw. On a full field zucchini crop. Journal of Sustainable Agriculture, 33(3), 349-358. https://doi.org/10.1080/10440040902773202
• Lazzeri, L., Curto, G., Leoni, O., & Dallavalle, E. (2004). Effects of glucosinolates and their enzymatic hydrolysis products via myrosinase on the root-knot nematode Meloidogyne incognita (Kofoid et White) Chitw. Journal of Agricultural and Food Chemistry, 52(22), 6703-6707. https://doi.org/10.1021/jf030776u
• Lim, H. W., Song, K. Y., Chon, J. W., Jeong, D., & Seo, K. H. (2019). Antimicrobial action of Raphanus raphanistrum subsp. sativus (radish) extracts against foodborne bacteria present in various milk products: A Preliminary Study. Journal of Dairy Science and Biotechnology, 37(3), 187-195. https://doi.org/10.22424/jmsb.2019.37.3.187
• Mazzola, M., Brown, J., Izzo, A. D., & Cohen, M. F. (2007). Mechanism of action and efficacy of seed meal-induced pathogen suppression differ in a Brassicaceae species and time-dependent manner. Phytopathology, 97(4), 454-460. https://doi.org/10.1094/PHYTO-97-4-0454
• Mazzola, M., Brown, J., Zhao, X., Izzo, A.D., & Fazio, G. (2009). Interaction of brassicaceous seed meal and apple rootstock on recovery of Pythium spp. and Pratylenchus penetrans from roots grown in replant soils. Plant Dis 93:51–57. https://doi.org/10.1094/PDIS-93-1-0051
• Melakeberhan, H., Mennan, S., Ngouajio, M., & Dudek, T. (2008). Effect of Meloidogyne hapla on multi-purpose use of oilseed radish (Raphanus sativus). Nematology, 10(3), 375-379.
• Muthusamy, B., & Shanmugam, G. (2020). Analysis of flavonoid content, antioxidant, antimicrobial and antibiofilm activity of in vitro hairy root extract of radish (Raphanus sativus L.). Plant Cell, Tissue and Organ Culture (PCTOC), 140, 619-633. https://doi.org/10.1007/s11240-019-01757-6.
• Pattison, A. B., Versteeg, C., Akiew, S., & Kirkegaard, J. (2006). Resistance of Brassicaceae plants to root-knot nematode (Meloidogyne spp.) in northern Australia. International Journal of Pest Management, 52(1), 53-62. https://doi.org/10.1080/09670870500424375
• Radwan, M. A., Farrag, S. A. A., Abu-Elamayem, M. M., & Ahmed, N. S. (2012). Biological control of the root-knot nematode, Meloidogyne incognita on tomato using bioproducts of microbial origin. Applied Soil Ecology, 56, 58-62. https://doi.org/10.1016/j.apsoil.2012.02.008
• Sahebani, N., Hadavi, N. S., & Zade, F. O. (2011). The effects of β-amino-butyric acid on resistance of cucumber against root-knot nematode, Meloidogyne javanica. Acta Physiologiae Plantarum, 33, 443-450. https://doi.org/10.1007/s11738-010-0564-0
• Sandler, L., Nelson, K. A., & Dudenhoeffer, C. J. (2015). Radish planting date and nitrogen rate for cover crop production and the impact on corn yields in upstate Missouri. Journal of Agricultural Science, 7(6), 1-13.
• Shalaby, M., Gad, S. B., Khalil, A. E., & El-Sherif, A. G. (2021). Nematicidal activity of seed powders of some ornamental plants against Meloidogyne incognita infecting pepper under greenhouse conditions. Journal of Plant Protection and Pathology, 12(8), 499-506. https://dx.doi.org/10.21608/jppp.2021.198191
• Sillva, G. S., Souza I. M., & Cutrim, F. A. (2002). Efeito da incorporação de sementes trituradas defeijão de porco ao solo sobre o parasitismo de Meloidogyne incognita em tomateiro. Fitopatologia brasileira, 27, 412-413. https://doi.org/10.1590/S0100-41582002000400014
• Sorribas, J. , & Ornat, C. (2011). Estrategias de control integrado de nematodos fitoparásitos. In: Andrés MF, Verdejo S (eds) Enfermedades causadas por nematodos fitoparásitos en España. Phytoma-SEF. Valencia, pp 115–127.
• Törün, B., Çoban, E. P., Biyik, H., & Barişik, E. (2017). Antimicrobial activity of Echinophora tenuifolia L. and Raphanus sativus L. extracts. Indian Journal of Pharmaceutical Education Research, 51(1), 136-43. DOI: 10.5530/ijper.51.3s.50
• Vallejo, F., Tomás-Barberán, F. A., & Ferreres, F. (2004). Characterisation of flavonols in broccoli (Brassica oleracea L. var. italica) by liquid chromatography–UV diode-array detection–electrospray ionisation mass spectrometry. Journal of chromatography A, 1054(1-2), 181-193. doi: 10.1016/j.chroma.2004.05.045.
• Zaidat, S. A. E., Mouhouche, F., Babaali, D., Abdessemed, N., De Cara, M., & Hammache, M. (2020). Nematicidal activity of aqueous and organic extracts of local plants against Meloidogyne incognita (Kofoid and White) Chitwood in Algeria under laboratory and greenhouse conditions. Egyptian Journal of Biological Pest Control, 30(1), 46. https://doi.org/10.1186/s41938-020-00242-z
• Zasada, I. A., & Ferris, H. (2004). Nematode suppression with brassicaceous amendments: application based upon glucosinolate profiles. Soil Biology and Biochemistry, 36(7), 1017-1024. https://doi.org/10.1016/j.soilbio.2003.12.014
• Zasada, I. A., Meyer, S. L. F., & Morra, M. J. (2009). Brassicaceous seed meals as soil amendments to suppress the plant-parasitic nematodes Pratylenchus penetrans and Meloidogyne incognita. Journal of Nematology, 41(3), 221.