Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture

Hamide Aydın; Hasan Pinar

doi:10.31015/jaefs.2026.1.17

Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture

Abstract

Phytophthora root and crown rot, caused by Phytophthora capsici, is one of the most destructive diseases limiting pepper (Capsicum annuum L.) production, particularly in regions where local cultivars such as Kahramanmaraş pepper are widely grown. This study aimed to rapidly develop P. capsici resistant Kahramanmaraş pepper type by integrating marker-assisted selection (MAS) with in vitro anther culture. A total of 198 genotypes belonging to five backcross-derived GM2 populations were developed by crossing a susceptible Kahramanmaraş pepper genotype with five resistant donor parents. Molecular screening was performed using the Phyto.5.2 SCAR marker to identify resistant genotypes at the seedling stage. Phenotypic variation in plant morphology and fruit-related traits was evaluated using principal component analysis (PCA). Genotypes identified as resistant were subsequently used as donor plants for anther culture to generate homozygous double haploid (DH) lines. Molecular analyses revealed that 83 genotypes were resistant to P. capsici. PCA results demonstrated substantial variation among genotypes in terms of yield and morphological traits, with resistant individuals distributed across diverse phenotypic backgrounds. Anther culture efficiency varied among hybrid combinations, with the highest success observed in the KP × P1 combination. The resulting DH lines exhibited wide variation in yield and fruit quality traits relevant to dried pepper production. Overall, the combined application of MAS, multivariate phenotypic analysis, and anther culture proved to be an effective and time-saving breeding strategy for developing P. capsici– resistant and agronomically superior pepper lines. The DH lines obtained in this study represent valuable genetic resources for future cultivar development and sustainable pepper production.

Keywords

Anther Culture, Double Haploid, Marker Assisted Selection, Pepper breeding, Phytophthora capsici

Project Number

FYL-2020-9928

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Details

Primary Language

English

Subjects

Plant Biotechnology in Agriculture

Journal Section

Research Article

Authors

Hamide Aydın ^*
0000-0002-0588-6192
Türkiye

Hasan Pinar
0000-0002-0811-8228
Türkiye

Early Pub Date

March 19, 2026

Publication Date

March 19, 2026

Submission Date

January 14, 2026

Acceptance Date

March 7, 2026

Published in Issue

Year 2026 Volume: 10 Number: 1

DOI

https://doi.org/10.31015/jaefs.2026.1.17

IZ

https://izlik.org/JA82DL63KS

APA

Aydın, H., & Pinar, H. (2026). Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture. International Journal of Agriculture Environment and Food Sciences, 10(1), 155-165. https://doi.org/10.31015/jaefs.2026.1.17

AMA

1.Aydın H, Pinar H. Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture. int. j. agric. environ. food sci. 2026;10(1):155-165. doi:10.31015/jaefs.2026.1.17

Chicago

Aydın, Hamide, and Hasan Pinar. 2026. “Development of Phytophthora Capsici–resistant Kahramanmaraş Pepper Lines Using Marker-Assisted Selection and Anther Culture”. International Journal of Agriculture Environment and Food Sciences 10 (1): 155-65. https://doi.org/10.31015/jaefs.2026.1.17.

EndNote

Aydın H, Pinar H (March 1, 2026) Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture. International Journal of Agriculture Environment and Food Sciences 10 1 155–165.

IEEE

[1]H. Aydın and H. Pinar, “Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture”, int. j. agric. environ. food sci., vol. 10, no. 1, pp. 155–165, Mar. 2026, doi: 10.31015/jaefs.2026.1.17.

ISNAD

Aydın, Hamide - Pinar, Hasan. “Development of Phytophthora Capsici–resistant Kahramanmaraş Pepper Lines Using Marker-Assisted Selection and Anther Culture”. International Journal of Agriculture Environment and Food Sciences 10/1 (March 1, 2026): 155-165. https://doi.org/10.31015/jaefs.2026.1.17.

JAMA

1.Aydın H, Pinar H. Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture. int. j. agric. environ. food sci. 2026;10:155–165.

MLA

Aydın, Hamide, and Hasan Pinar. “Development of Phytophthora Capsici–resistant Kahramanmaraş Pepper Lines Using Marker-Assisted Selection and Anther Culture”. International Journal of Agriculture Environment and Food Sciences, vol. 10, no. 1, Mar. 2026, pp. 155-6, doi:10.31015/jaefs.2026.1.17.

Vancouver

1.Hamide Aydın, Hasan Pinar. Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture. int. j. agric. environ. food sci. 2026 Mar. 1;10(1):155-6. doi:10.31015/jaefs.2026.1.17

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Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture

Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture

Abstract

Keywords

Project Number

References

Details

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Subjects

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Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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