In vitro salinity screening of garlic (Allium sativum L.) via shoot tip culture under NaCl stress

Şerife Düzen; Mirac Şahin; Hatıra Taşkın; Gökhan Baktemur

doi:10.31015/jaefs.2026.3.2

In vitro salinity screening of garlic (Allium sativum L.) via shoot tip culture under NaCl stress

Abstract

Salinity is one of the major abiotic stresses limiting plant growth and agricultural productivity worldwide. This study investigated the effects of NaCl induced salinity stress on the in vitro growth of garlic (Allium sativum L.) plantlets derived from shoot tip culture. Shoot tip explants of Taşköprü garlic were cultured on Murashige and Skoog (MS) medium supplemented with 0, 100, 200, and 300 mM NaCl. Shoot fresh weight (SFW), shoot dry weight (SDW), shoot length (SL), dry matter content (DMC), and infection rate were evaluated on the 7th, 14th, 21st, and 28th days of culture. NaCl stress generally reduced growth parameters in a concentration dependent manner. While 100 mM NaCl caused moderate inhibition, maintaining growth parameters at approximately 77-80% of control values, concentrations of 200 mM and above resulted in severe suppression of shoot growth and biomass accumulation. Shoot length exhibited the strongest linear response to NaCl concentration on the 28th day (y = -0.143x + 55.82; R² = 0.917), confirming its reliability as a salinity stress indicator in garlic. These findings confirm the salt sensitivity of garlic under in vitro conditions and demonstrate that shoot tip culture is an effective system for salinity tolerance screening. Shoot length and shoot fresh weight are proposed as key morphological indicators for evaluating NaCl stress responses in garlic.

Keywords

Allium sativum, In vitro, NaCl stress, Salinity tolerance, Shoot tip culture

References

Abdel-Farid, I. B., Marghany, M. R., Rowezek, M. M., & Sheded, M. G. (2020). Effect of Salinity Stress on Growth and Metabolomic Profiling of Cucumis sativus and Solanum lycopersicum. Plants, 9(11), 1626. https://doi.org/10.3390/plants9111626
Adem, E. İ., Evlice, A. K., Kara, E., Taşkın, H., & Baktemur, G. (2025). Impact of Drought Stress on Growth, Phenolic Content, and Antioxidant Activity in Tomato (Solanum lycopersicum L.) Cultivars under In Vitro Conditions. Biology Bulletin, 52(11), 330. https://doi.org/10.1134/S1062359025610936
Ahmad, J., Wang, H., Song, J., Nuerawuti, M., Zhang, X., Yang, W., ... & Li, X. X. (2021). The Tolerance of an Extensive Collection of Garlic (Allium sativum L.) Germplasms to Salt Stress–A Sustainable Solution to Salt Stress. Applied Ecology & Environmental Research, 19(3). http://dx.doi.org/10.15666/aeer/1903_22812303
Al-Safadi, B., & Faoury, H. (2004). Evaluation of salt tolerance in Garlic (Allium sativum L.) cultivars using in vitro techniques. Advances in Horticultural Science, 115-120. https://www.jstor.org/stable/42882324
Bekiş Hasdeniz, P., Ergül, B., Kara, E., & Baktemur, G. (2025). Evaluation of the Effects of Cobalt Concentrations on the Growth of Bean (Phaseolus vulgaris) Varieties under In vitro Conditions. International Journal of Agriculture Environment and Food Sciences, 999-1008. https://doi.org/10.31015/2025.4.2
Benke, A. P., Krishna, R., Khandagale, K., Gawande, S., Shelke, P., Dukare, S., ... & Mahajan, V. (2023). Efficient elimination of viruses from garlic using a combination of shoot meristem culture, thermotherapy, and chemical treatment. Pathogens, 12(1), 129. https://doi.org/10.3390/pathogens12010129
Çavuşoğlu, D. (2023). Modulation of NaCl-induced osmotic, cytogenetic, oxidative and anatomic damages by coronatine treatment in onion (Allium cepa L.). Scientific Reports, 13(1), 1580. https://doi.org/10.1038/s41598-023-28849-w
Demirel, M., Çilesiz, Y., Kara, E., & Baktemur, G. (2025). Effect of Salinity Stress on Plant Growth Parameters and Antioxidant Activity In Some Cucumber Cultivars (Cucumis sativus L.) Grown Under In Vitro Conditions. Turkish Journal of Agricultural and Natural Sciences, 12(2), 531-542. https://doi.org/10.30910/turkjans.1641798
Ergül, B., Kara, E., Düzen, Ş., Evlice, A. K., Taşkın, H., & Baktemur, G. (2026). In vitro assessment of drought tolerance in summer squash (Cucurbita pepo L.) cultivars. Acta Scientiarum Polonorum Hortorum Cultus, 25(1), 55-66. https://doi.org/10.24326/asphc.2026.5590
FAO. (2024). Global status of salt-affected soils—main report. Rome.

Gonzalez, R. E., Soto, V. C., Sance, M. M., Camargo, A. B., & Galmarini, C. R. (2009). Variability of solids, organosulfur compounds, pungency and health-enhancing traits in garlic (Allium sativum L.) cultivars belonging to different ecophysiological groups. Journal of Agricultural and Food Chemistry, 57(21), 10282-10288.
Greedharry, P., Boodhram, K. I. D., & Koyelas, C. (2024). In vitro Propagation of Garlic (Allium sativum L) from Meristem Culture. Current Agriculture Research Journal, 12(2). https://doi.org/10.12944/CARJ.12.2.10
Qaseem, M. F., Qureshi, R., & Shaheen, H. (2019). Effects of pre-anthesis drought, heat and their combination on the growth, yield and physiology of diverse wheat (Triticum aestivum L.) genotypes varying in sensitivity to heat and drought stress. Scientific reports, 9(1), 6955. https://doi.org/10.1038/s41598-019-43477-z
Kara, E., Taşkın, H., & Baktemur, G. (2024). In Vitro Koşullarda Besin Ortamına Eklenen Farklı Konsantrasyonlarda Tuz’un Domates (Solanum lycopersicum L.) Bitkisinin Gelişimi Üzerine Etkilerinin Belirlenmesi. ISPEC Journal of Agricultural Sciences, 8(2), 301-309. https://doi.org/10.5281/zenodo.11123176
Kara, E. (2025). Polietilen Glikol (PEG) ile Oluşturulan Kuraklık Stresine Karşı Farklı Turp (Raphanus sativus L.) Çeşitlerinin İn Vitro Tepkileri. Ordu Üniversitesi Bilim ve Teknoloji Dergisi, 15(2), 372-386. https://doi.org/10.54370/ordubtd.1804349
Kara, E., Taşkın, H., Shimira, F., Karaköy, T., & Baktemur, G. (2025). Phenotypic responses of Capsicum annuum L. to salinity stress under in vitro conditions. Vegetos, 1-8. https://doi.org/10.1007/s42535-025-01412-w
Kara, E. (2026). Dose dependent phytotoxic effects of Cd, Ni, and Cu on cauliflower in vitro. International Journal of Agriculture Environment and Food Sciences, (Advanced Online Publication), 300-308. https://doi.org/10.31015/jaefs.2026.1.32
Liu, C., Jiang, X., & Yuan, Z. (2024). Plant responses and adaptations to salt stress: A review. Horticulturae, 10(11), 1221. https://doi.org/10.3390/horticulturae10111221
Mancak, Z., & Baktemur, G. (2023). Effects of Nutrient Media Including Heavy Metals at Different Concentrations in In Vitro Conditions on the Growth of Squash (Cucurbita pepo L.). Türk Doğa ve Fen Dergisi, 12(1), 30-36. https://doi.org/10.46810/tdfd.1225384
Mohammadi Alagoz, S., Hadi, H., Toorchi, M., Pawłowski, T. A., Asgari Lajayer, B., Price, G. W., ... & Astatkie, T. (2023). Morpho-physiological responses and growth indices of triticale to drought and salt stresses. Scientific Reports, 13(1), 8896. https://doi.org/10.1038/s41598-023-36119-y
Mohammed, S. P., Yen, J. Y., Hsu, Y. C., Chou, H. Y., Natarajan, S., & Eybishitz, A. (2025). Integrative Trait Analysis for Enhancing Heat Stress Resilience in Tomato (Solanum lycopersicum L.): A Focus on Root, Physiological, and Yield Adaptations. Plants, 14(4), 533. https://doi.org/10.3390/plants14040533
Muchate, N. S., Rajurkar, N. S., Suprasanna, P., & Nikam, T. D. (2019). NaCl induced salt adaptive changes and enhanced accumulation of 20-hydroxyecdysone in the in vitro shoot cultures of Spinacia oleracea (L.). Scientific reports, 9(1), 12522. https://doi.org/10.1038/s41598-019-48737-6
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nana, J. B., Abd El Baki, H. M., & Fujimaki, H. (2024). Determining Drought and Salinity Stress Response Function for Garlic. Soil Systems, 8(2), 59. https://doi.org/10.3390/soilsystems8020059
Parreño, R., Rodríguez-Alcocer, E., Martínez-Guardiola, C., Carrasco, L., Castillo, P., Arbona, V., ... & Candela, H. (2023). Turning garlic into a modern crop: State of the art and perspectives. Plants, 12(6), 1212. https://doi.org/10.3390/plants12061212
Romo-Pérez, M. L., Weinert, C. H., Egert, B., Kulling, S. E., & Zörb, C. (2024). The tale of two Ions Na+ and Cl−: unraveling onion plant responses to varying salt treatments. BMC Plant Biology, 24(1), 1022. https://doi.org/10.1186/s12870-024-05719-9
Sanwal, S. K., Kumar, A., Kundu, A., Kesh, H., & Kumar, A. (2026). Genotypic variation in garlic (Allium sativum L.) for salinity tolerance: role of antioxidant enzymes and bulb ionic ratios in yield maintenance. Frontiers in Plant Science, 17, 1825262. https://doi.org/10.3389/fpls.2026.1825262
Shang, A., Cao, S. Y., Xu, X. Y., Gan, R. Y., Tang, G. Y., Corke, H., ... & Li, H. B. (2019). Bioactive compounds and biological functions of garlic (Allium sativum L.). Foods, 8(7), 246. https://doi.org/10.3390/foods8070246
Singh, A. (2021). In vitro studies of salinity stress response in allium sativum. J Agric Sci B ot. 5(7):1-7.
Solouki, A., Berna-Sicilia, J. Á., Martinez-Alonso, A., Ortiz-Delvasto, N., Bárzana, G., & Carvajal, M. (2023). Onion plants (Allium cepa L.) react differently to salinity levels according to the regulation of aquaporins. Heliyon, 9(3). https://doi.org/10.1016/j.heliyon.2023.e13815
Tang, W., Liang, L., Yang, H., Yu, X., Ye, X., Xie, Y., ... & Tang, Y. (2024). Exogenous salicylic acid reduces cadmium content in spinach (Spinacia oleracea L.) shoots under cadmium stress. BMC Plant Biology, 24(1), 1226. https://doi.org/10.1186/s12870-024-05948-y
Taşkın, H., Baktemur, G., Kurul, M., & Büyükalaca, S. (2013). Use of tissue culture techniques for producing virus‐free plant in garlic and their identification through real‐time PCR. The Scientific World Journal, 2013(1), 781282. https://doi.org/10.1155/2013/781282
Uçar, M., Kara, E., Şahin, A., Taskin, H., & Baktemur, G. (2026). Assessment of salt tolerance in spinach under in vitro condition. Chilean journal of agricultural research, 86(1), 77-85. http://dx.doi.org/10.4067/s0718-58392026000100077
Waheed, A., Zhuo, L., Wang, M., Hailiang, X., Tong, Z., Wang, C., & Aili, A. (2024). Integrative mechanisms of plant salt tolerance: Biological pathways, phytohormonal regulation, and technological innovations. Plant Stress, 14, 100652. https://doi.org/10.1016/j.stress.2024.100652
Waqas, M. A., Kaya, C., Riaz, A., Farooq, M., Nawaz, I., Wilkes, A., & Li, Y. (2019). Potential mechanisms of abiotic stress tolerance in crop plants induced by thiourea. Frontiers in plant science, 10, 1336. https://doi.org/10.3389/fpls.2019.01336
Zhou, X., Dou, Y., Huang, X., Li, G., Zhang, H., Jiang, D., ... & Zhang, C. (2021). Using principal component analysis and RNA-seq to identify candidate genes that control salt tolerance in garlic (Allium sativum L.). Agronomy, 11(4), 691. https://doi.org/10.3390/agronomy11040691

Details

Primary Language

English

Subjects

Agricultural Biotechnology (Other)

Journal Section

Research Article

Authors

Şerife Düzen
0009-0006-6203-5075
Türkiye

Mirac Şahin
0009-0000-1817-1922
Türkiye

Hatıra Taşkın
0000-0002-1784-4731
Türkiye

Gökhan Baktemur ^*
0000-0002-0362-5108
Türkiye

Early Pub Date

August 29, 2026

Publication Date

-

Submission Date

June 1, 2026

Acceptance Date

July 26, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.31015/jaefs.2026.3.2

IZ

https://izlik.org/JA76AH83SD

APA

Düzen, Ş., Şahin, M., Taşkın, H., & Baktemur, G. (2026). In vitro salinity screening of garlic (Allium sativum L.) via shoot tip culture under NaCl stress. International Journal of Agriculture Environment and Food Sciences, Advanced Online Publication, 542-550. https://doi.org/10.31015/jaefs.2026.3.2

AMA

1.Düzen Ş, Şahin M, Taşkın H, Baktemur G. In vitro salinity screening of garlic (Allium sativum L.) via shoot tip culture under NaCl stress. int. j. agric. environ. food sci. 2026;(Advanced Online Publication):542-550. doi:10.31015/jaefs.2026.3.2

Chicago

Düzen, Şerife, Mirac Şahin, Hatıra Taşkın, and Gökhan Baktemur. 2026. “In Vitro Salinity Screening of Garlic (Allium Sativum L.) via Shoot Tip Culture under NaCl Stress”. International Journal of Agriculture Environment and Food Sciences, no. Advanced Online Publication: 542-50. https://doi.org/10.31015/jaefs.2026.3.2.

EndNote

Düzen Ş, Şahin M, Taşkın H, Baktemur G (August 1, 2026) In vitro salinity screening of garlic (Allium sativum L.) via shoot tip culture under NaCl stress. International Journal of Agriculture Environment and Food Sciences Advanced Online Publication 542–550.

IEEE

[1]Ş. Düzen, M. Şahin, H. Taşkın, and G. Baktemur, “In vitro salinity screening of garlic (Allium sativum L.) via shoot tip culture under NaCl stress”, int. j. agric. environ. food sci., no. Advanced Online Publication, pp. 542–550, Aug. 2026, doi: 10.31015/jaefs.2026.3.2.

ISNAD

Düzen, Şerife - Şahin, Mirac - Taşkın, Hatıra - Baktemur, Gökhan. “In Vitro Salinity Screening of Garlic (Allium Sativum L.) via Shoot Tip Culture under NaCl Stress”. International Journal of Agriculture Environment and Food Sciences. Advanced Online Publication (August 1, 2026): 542-550. https://doi.org/10.31015/jaefs.2026.3.2.

JAMA

1.Düzen Ş, Şahin M, Taşkın H, Baktemur G. In vitro salinity screening of garlic (Allium sativum L.) via shoot tip culture under NaCl stress. int. j. agric. environ. food sci. 2026;:542–550.

MLA

Düzen, Şerife, et al. “In Vitro Salinity Screening of Garlic (Allium Sativum L.) via Shoot Tip Culture under NaCl Stress”. International Journal of Agriculture Environment and Food Sciences, no. Advanced Online Publication, Aug. 2026, pp. 542-50, doi:10.31015/jaefs.2026.3.2.

Vancouver

1.Şerife Düzen, Mirac Şahin, Hatıra Taşkın, Gökhan Baktemur. In vitro salinity screening of garlic (Allium sativum L.) via shoot tip culture under NaCl stress. int. j. agric. environ. food sci. 2026 Aug. 1;(Advanced Online Publication):542-50. doi:10.31015/jaefs.2026.3.2

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