A first insight into genetic diversity of Jerusalem artichoke accessions collected from different regions of Turkey assessed by ISSR markers
Abstract
The study aimed to assess genetic diversity among Jerusalem artichoke (Helianthus tuberosus L.) accessions using ISSR (Inter Simple Sequence Repeat) markers. Twenty-five Jerusalem artichoke accessions collected from the different regions of Turkey have been used for molecular analysis. Nine primers used in the study produced a total of 62 bands. A total 57 of these are polymorphic. 57 of them resulted in polymorphic. Allele's average number was 6.33. Polymorphic information content (PIC) varied between 0.219 and 0.340. Examined accessions were classified into two main groups in a dendrogram. Genetic similarities varied from 0.001 to 0.815, with an average of 0.292. Also, Principal Coordinate Analysis (PCoA) of molecular data was conducted. These findings present valuable diversity data for recognizing the Jerusalem artichoke germplasm, preserving genetic richness, and determining parents for genetic enhancement.
Keywords
Genetic diversity, Gene-Pool, Helianthus tuberosus L., ISSR
Supporting Institution
Research Fund of the Erciyes University
Project Number
FHD-2018-8595
References
- Balkaya, A. and Yanmaz, R. (2001). Conservation Facilities of Plant Genetic Resources and Working Systems of Seed Gene Banks. International Journal of Environment, 39: 25-30.
- Broughton, W.J., Hernandez, G., Blair, M., Beebe, S., Gepts, P., Vanderleyden, J. (2003). Beans (Phaseolus vulgaris) Model Food Legumes. Plant and Soil, 252: 55-128
- Brown, H.D. (1989). Core Collection: A Practical Approach to Genetic Resources Management, Genome, 31(2), 818-824.
- Hammer, O., Harper D.A.T., Ryan, P.D. (2001). PAST: Paleontological Statistic software package for education and data analysis. Paleontologia Eletronica 4 (1): 1-9
- Hanci F. and Tuncer G. (2019). The investıgatıon of the variation in tuber characteristics of jerusalem artichoke collected from different provinces of Turkey", International Congress on Vocational and Technical Sciences - V, Cairo, Egypt, 24-34.
- Heiser, C.B.Jr. (1978). Taxonomy of Helianthus and the origin of domesticated sunflower. In W.Fehr (ed.) Sunflower Sci. And Technology. Agronomy, 19: 31-53.
- Hildebrand, C.E., Torney, D.C., Wagner R.P. (1992). Informativeness of polymorphic DNA markers. Los Alamos Science, 20: 100–102.
- Jung, W.Y., Lee, S.S., Kim C.W., Kim H.S., Min, S.R., Moon J.S., Kwon S.Y., Jeon, J.H., Cho. H.S. 2014. RNA-seq analysis and de novo transcriptome assembly of Jerusalem artichoke (Helianthus tuberosus). PLoS One, 9: e111982. https://doi.org/10.1371/journal.pone.0111982
- Karaca, M., Saha, S.S., Zipf, A., Jenkins J.N., Lang. D.J. (2002). Genetic diversity among forage bermudagrass (Cynodon spp.): Evidence from chloroplast and nuclear DNA fingerprinting. Crop Science, 42: 2118- 2127. https://doi.org/10.2135/cropsci2002.2118
- Kays, S.J., Nottingham, S.F. (2007). Biology and Chemistry of Jerusalem artichoke: Helianthus tuberosus L., CRC press, Boca Raton. https://doi.org/10.1201/9781420044966
