Research Article
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A first insight into genetic diversity of Jerusalem artichoke accessions collected from different regions of Turkey assessed by ISSR markers

Year 2021, , 651 - 655, 15.12.2021
https://doi.org/10.31015/jaefs.2021.4.26

Abstract

The study aimed to assess genetic diversity among Jerusalem artichoke (Helianthus tuberosus L.) accessions using ISSR (Inter Simple Sequence Repeat) markers. Twenty-five Jerusalem artichoke accessions collected from the different regions of Turkey have been used for molecular analysis. Nine primers used in the study produced a total of 62 bands. A total 57 of these are polymorphic. 57 of them resulted in polymorphic. Allele's average number was 6.33. Polymorphic information content (PIC) varied between 0.219 and 0.340. Examined accessions were classified into two main groups in a dendrogram. Genetic similarities varied from 0.001 to 0.815, with an average of 0.292. Also, Principal Coordinate Analysis (PCoA) of molecular data was conducted. These findings present valuable diversity data for recognizing the Jerusalem artichoke germplasm, preserving genetic richness, and determining parents for genetic enhancement.

Supporting Institution

Research Fund of the Erciyes University

Project Number

FHD-2018-8595

References

  • Balkaya, A. and Yanmaz, R. (2001). Conservation Facilities of Plant Genetic Resources and Working Systems of Seed Gene Banks. International Journal of Environment, 39: 25-30.
  • Broughton, W.J., Hernandez, G., Blair, M., Beebe, S., Gepts, P., Vanderleyden, J. (2003). Beans (Phaseolus vulgaris) Model Food Legumes. Plant and Soil, 252: 55-128
  • Brown, H.D. (1989). Core Collection: A Practical Approach to Genetic Resources Management, Genome, 31(2), 818-824.
  • Hammer, O., Harper D.A.T., Ryan, P.D. (2001). PAST: Paleontological Statistic software package for education and data analysis. Paleontologia Eletronica 4 (1): 1-9
  • Hanci F. and Tuncer G. (2019). The investıgatıon of the variation in tuber characteristics of jerusalem artichoke collected from different provinces of Turkey", International Congress on Vocational and Technical Sciences - V, Cairo, Egypt, 24-34.
  • Heiser, C.B.Jr. (1978). Taxonomy of Helianthus and the origin of domesticated sunflower. In W.Fehr (ed.) Sunflower Sci. And Technology. Agronomy, 19: 31-53.
  • Hildebrand, C.E., Torney, D.C., Wagner R.P. (1992). Informativeness of polymorphic DNA markers. Los Alamos Science, 20: 100–102.
  • Jung, W.Y., Lee, S.S., Kim C.W., Kim H.S., Min, S.R., Moon J.S., Kwon S.Y., Jeon, J.H., Cho. H.S. 2014. RNA-seq analysis and de novo transcriptome assembly of Jerusalem artichoke (Helianthus tuberosus). PLoS One, 9: e111982. https://doi.org/10.1371/journal.pone.0111982
  • Karaca, M., Saha, S.S., Zipf, A., Jenkins J.N., Lang. D.J. (2002). Genetic diversity among forage bermudagrass (Cynodon spp.): Evidence from chloroplast and nuclear DNA fingerprinting. Crop Science, 42: 2118- 2127. https://doi.org/10.2135/cropsci2002.2118
  • Kays, S.J., Nottingham, S.F. (2007). Biology and Chemistry of Jerusalem artichoke: Helianthus tuberosus L., CRC press, Boca Raton. https://doi.org/10.1201/9781420044966
  • Mornkham, T., Wangsomnuk, P.P., Mo, X.C., Francisco, F.O., Gao, L.Z., Kurzweil, H. (2016). Development and characterization of novel EST-SSR markers and their application for genetic diversity analysis of Jerusalem artichoke (Helianthus tuberosus L.), Genetics and Molecular Research, 15 (4): 1-16. https://doi.org/10.4238/gmr15048857
  • Nagy S., Pozcai P., Cernak I.A., Gorji M., Hegedüs G., Taller J. (2012). PICcalc: An online program to calculate polymorphic information content for molecular genetic studies. Biochemical Genetics, 50: 670–673. https://doi.org/10.1007/s10528-012-9509-1
  • Tan, A. (2010). State of Plant Genetic Resources for Food and Agriculture. Second Report of Turkey on Conservation and Sustainable Utilization of Plant Genetic Resources for Food and Agriculture, Meta Basım. Bornova, Izmir, Turkey .
  • Vardar-Kanlıtepe, Ç., Aras, S., Cansaran-Duman, D. (2010). Application of the Molecular Marker and Gene Transfer in Plant Breeding. Turkish Bulletin of Hygiene and Experimental Biology, 67:(1) 33-43
  • Wangsomnuk, P.P., Khampa, S.J., Srivong, T., Patanothai, A., Fu, Y.B. (2011). Assessing genetic structure and relatedness of Jerusalem artichoke (Helianthus tuberosus L.) germplasm with RAPD, ISSR and SRAP markers. American Journal of Plant Science, 2:753-764. https://doi.org/10.4236/ajps.2011.26090
  • Wangsomnuk, P.P., Khampa, S., Jogloy, S., Mornkham, T., Ruttawat, B., Patanothai, A., Fu, Y.B. (2011). Genetic diversity of worldwide Jerusalem artichoke (Helianthus tuberosus) germplasm as revealed by RAPD markers. Genetics and molecular research: GMR, 10 4, 4012-25 . https://doi.org/10.4238/2011
  • Wouw, M.V.D., Hintum, T.V., Kik, C., Treuren, R.V., Visser, B. (2010). Genetic diversity trends in twentieth century crop cultivars: a meta-analysis. Theorical and Applied Genetics, 120: 1241-1252. https://doi.org/10.1007/s00122-009-1252-6
Year 2021, , 651 - 655, 15.12.2021
https://doi.org/10.31015/jaefs.2021.4.26

Abstract

Project Number

FHD-2018-8595

References

  • Balkaya, A. and Yanmaz, R. (2001). Conservation Facilities of Plant Genetic Resources and Working Systems of Seed Gene Banks. International Journal of Environment, 39: 25-30.
  • Broughton, W.J., Hernandez, G., Blair, M., Beebe, S., Gepts, P., Vanderleyden, J. (2003). Beans (Phaseolus vulgaris) Model Food Legumes. Plant and Soil, 252: 55-128
  • Brown, H.D. (1989). Core Collection: A Practical Approach to Genetic Resources Management, Genome, 31(2), 818-824.
  • Hammer, O., Harper D.A.T., Ryan, P.D. (2001). PAST: Paleontological Statistic software package for education and data analysis. Paleontologia Eletronica 4 (1): 1-9
  • Hanci F. and Tuncer G. (2019). The investıgatıon of the variation in tuber characteristics of jerusalem artichoke collected from different provinces of Turkey", International Congress on Vocational and Technical Sciences - V, Cairo, Egypt, 24-34.
  • Heiser, C.B.Jr. (1978). Taxonomy of Helianthus and the origin of domesticated sunflower. In W.Fehr (ed.) Sunflower Sci. And Technology. Agronomy, 19: 31-53.
  • Hildebrand, C.E., Torney, D.C., Wagner R.P. (1992). Informativeness of polymorphic DNA markers. Los Alamos Science, 20: 100–102.
  • Jung, W.Y., Lee, S.S., Kim C.W., Kim H.S., Min, S.R., Moon J.S., Kwon S.Y., Jeon, J.H., Cho. H.S. 2014. RNA-seq analysis and de novo transcriptome assembly of Jerusalem artichoke (Helianthus tuberosus). PLoS One, 9: e111982. https://doi.org/10.1371/journal.pone.0111982
  • Karaca, M., Saha, S.S., Zipf, A., Jenkins J.N., Lang. D.J. (2002). Genetic diversity among forage bermudagrass (Cynodon spp.): Evidence from chloroplast and nuclear DNA fingerprinting. Crop Science, 42: 2118- 2127. https://doi.org/10.2135/cropsci2002.2118
  • Kays, S.J., Nottingham, S.F. (2007). Biology and Chemistry of Jerusalem artichoke: Helianthus tuberosus L., CRC press, Boca Raton. https://doi.org/10.1201/9781420044966
  • Mornkham, T., Wangsomnuk, P.P., Mo, X.C., Francisco, F.O., Gao, L.Z., Kurzweil, H. (2016). Development and characterization of novel EST-SSR markers and their application for genetic diversity analysis of Jerusalem artichoke (Helianthus tuberosus L.), Genetics and Molecular Research, 15 (4): 1-16. https://doi.org/10.4238/gmr15048857
  • Nagy S., Pozcai P., Cernak I.A., Gorji M., Hegedüs G., Taller J. (2012). PICcalc: An online program to calculate polymorphic information content for molecular genetic studies. Biochemical Genetics, 50: 670–673. https://doi.org/10.1007/s10528-012-9509-1
  • Tan, A. (2010). State of Plant Genetic Resources for Food and Agriculture. Second Report of Turkey on Conservation and Sustainable Utilization of Plant Genetic Resources for Food and Agriculture, Meta Basım. Bornova, Izmir, Turkey .
  • Vardar-Kanlıtepe, Ç., Aras, S., Cansaran-Duman, D. (2010). Application of the Molecular Marker and Gene Transfer in Plant Breeding. Turkish Bulletin of Hygiene and Experimental Biology, 67:(1) 33-43
  • Wangsomnuk, P.P., Khampa, S.J., Srivong, T., Patanothai, A., Fu, Y.B. (2011). Assessing genetic structure and relatedness of Jerusalem artichoke (Helianthus tuberosus L.) germplasm with RAPD, ISSR and SRAP markers. American Journal of Plant Science, 2:753-764. https://doi.org/10.4236/ajps.2011.26090
  • Wangsomnuk, P.P., Khampa, S., Jogloy, S., Mornkham, T., Ruttawat, B., Patanothai, A., Fu, Y.B. (2011). Genetic diversity of worldwide Jerusalem artichoke (Helianthus tuberosus) germplasm as revealed by RAPD markers. Genetics and molecular research: GMR, 10 4, 4012-25 . https://doi.org/10.4238/2011
  • Wouw, M.V.D., Hintum, T.V., Kik, C., Treuren, R.V., Visser, B. (2010). Genetic diversity trends in twentieth century crop cultivars: a meta-analysis. Theorical and Applied Genetics, 120: 1241-1252. https://doi.org/10.1007/s00122-009-1252-6
There are 17 citations in total.

Details

Primary Language English
Subjects Botany, Horticultural Production
Journal Section Research Articles
Authors

Fatih Hancı 0000-0002-2015-0351

Project Number FHD-2018-8595
Publication Date December 15, 2021
Submission Date March 11, 2021
Acceptance Date December 20, 2021
Published in Issue Year 2021

Cite

APA Hancı, F. (2021). A first insight into genetic diversity of Jerusalem artichoke accessions collected from different regions of Turkey assessed by ISSR markers. International Journal of Agriculture Environment and Food Sciences, 5(4), 651-655. https://doi.org/10.31015/jaefs.2021.4.26

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