In vitro antioxidant activity and carbonic anhydrase inhibitory features of Ferula communis extracts

Year 2021, , 592 - 598, 15.12.2021

Fatma Aydın , Zeynep Aleyna Kahraman , Emir Alper Türkoğlu , Müslüm Kuzu , Zeki Severoğlu

https://doi.org/10.31015/jaefs.2021.4.19

Cited By: 3

Abstract

Carbonic anhydrases (CAs; EC 4.2.1.1) are essential family of metalloenzymes which catalyze the interconversion between carbon dioxide (CO2) and bicarbonate (HCO3-) in all organisms of three-domains of life. Huge amounts of attempts related to catalytic activity of CAs have been widely expanded to treat many clinical diseases. This study aimed to determine in-vitro antioxidant activities and human CA I (hCA I) and II (hCA II) inhibitory properties of Ferula communis extracts. Among all extracts of F. communis, the hexane extract has showed the best inhibitory profile on hCA I and II with IC50 values 8.68 µg/mL and 28 µg/mL and Ki values 2.026 µg/mL and 11.6 µg/mL, respectively. All extracts showed mild to moderate antioxidant activity. According to the results of DPPH assay, ethanol-water extract showed the highest activity with IC50: 0.1128±0.0066 value. Chloroform extract showed the highest activity on CUPRAC assay with the value of 1.305±0.037 mM Trolox equivalent/mg extract. However, further analytical, in-vivo and clinical studies are needed to confirm the activities of F. communis.

Keywords

Antioxidant activity, Carbonic anhydrase, Ferula communis, Inhibition

Supporting Institution

TÜBİTAK and Scientific Research Project Fund of the University of Health Sciences Turkey

Project Number

TUBITAK 2209-A University Students Research Projects Support Program for Zeynep Aleyna KAHRAMAN and Scientific Research Project Fund of the University of Health Sciences Turkey under project number 2018/011.

Thanks

We also would like to thank our collaborator, Assoc. Prof. Dr. Turgut TAŞKIN, for his excellent recommendations.

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