Facilitation of olive harvest by microbial indole acetic acid and an enzyme mixture

Year 2018, Volume: 2 Issue: 3, 88 - 92, 20.09.2018

Aynur Gul Karahan , Neylan Cetin Mehmet Lutfu Cakmakci

https://doi.org/10.31015/jaefs.18014

Abstract

In this study, the effect of indole acetic acid (IAA) and/or a commercial enzyme mixture on the olive harvest was analyzed. IAA was produced by Gibberella fujikuroi. IAA (1500 and 3000 ppm) and/or the commercial enzyme preparation (1%) was mixed and applied to the olive trees before (3 weeks and 1 week) the harvest. The changes in protein, pectin and cellulose contents of fruits were determined monthly. While the cellulose and protein contents showed a decrease in 2 periods of 3 months (July-September and October-December), pectin contents increased in the same period. IAA and/or enzyme applications did not lead to any significant changes in the cellulose and protein contents of the fruit (p>0,001). However, the pectin amount in the trees where the enzyme application was performed before the harvest, showed a tendency to fall. The most convenient application with regard to the fruit and leaf fall was 3000 ppm IAA+1% enzyme application 3 weeks before harvest. While the fruit fall amount increased more when compared to the control and other groups with this application, the leaf fall decreased significantly.

Keywords

Gibberella fujikuroi, Pectinolytic Enzymes, Protein, Pectin

References

• AOAC (1990). Official Methods of Analysis of AOAC International. In Association of Official Analysis Chemists International pp. 1058–1059.
• ASTM (1980). American Society for Testing Materials, Standard Test Method for Holo Cellulose, a-Cellulose and Hemi Celluloses. ASTM D1103, D1104 pp.269–270.
• Barut, E. (1995). The use of growth regulators for garden plants in future. Derim, 11(3), 141–144.
• Ben, T.Y., Wodner, M. (1993). Chemical loosening of olive pedicels for mechanical harvesting. Acta Horticulturae, 356, 297–301.
• Bulbul, E. (2008). Every aspect of olive. Inkilap Bookstore and Press Facilities, Istanbul, Turkey, pp. 240.
• Castillo-Ruiz, F.J., Jiménez-Jiménez, F., Blanco-Roldán, G.L., Sola-Guirado, R.R., Agüera-Vega, J., Castro-Garcia, S. (2015). Analysis of fruit and oil quantity and quality distribution in high-density olive trees in order to improve the mechanical harvesting process. Spanish Journal of Agricultural Research, 13(2), 1–8.
• Cemeroglu, B. (1976). Jam, marmalade, jelly production technology and analysis methods. Ayyıldız Press, Ankara, Turkey, pp. 95.
• Cakmakci, M.L. (1981). Studies on the effects of some synthetic plant hormones on the development of Rhizobium phaseoli strains and the effect of indole acetic acid on the competitiveness of these strains. Postdoctoral Thesis (Assoc. Prof.), Ankara University, Faculty of Agriculture, Chair of Microbiology, Ankara, Turkey, pp. 105.
• Cavusoglu, A. (1973). A study on the use of Ethrel for reducing attachment power of olives and facilitating harvesting. A project of Olive Research Institute, İzmir, Turkey.
• Cavusoglu, A. (1977). The use of certain chemical materials in the harvest of Memecik olive variety. A project of Olive Research Institute, İzmir, Turkey.
• Davies, P.J. (2010). The Plant Hormones Biosynthesis, Signal Transduction, Action! In P. J. Davies, ed. Plant Hormones. Springer Science+Business Media, New York.
• Ergun, N. (1997). Production of endogenous growth hormones (oxine, gibberellin, cytokinin and abscisic acid) by some algae and lichen species. MSc Thesis. Mustafa Kemal University Institute of Science, Hatay, Turkey.
• Fernandez-Bolaños, J., Rodriquez, R., Guillien, R., Jimenez, A., Heredia, A. (1995). Activity of cell wall associated enzymes in ripening olive fruit. Physiologia Plantarum, 93, 651–658.
• Fernández-Bolaños, J., Heredia, A., Vioque, B., Castellano, J.M., Guillien, R. (1997). Changes in cell-wall-degrading enzyme activities in stored olives in relation to respiration and ethylene production - Influence of exogenous ethylene. Zeitschrift fur Lebensmittel-Untersuchung Und-Forschung A-Food Research and Technology, 204, 293–299.
• Jaroszuk-Ściseł, J., Kurek, E., Trytek, M. (2014). Efficiency of indoleacetic acid, gibberellic acid and ethylene synthesized in vitro by Fusarium culmorum strains with different effects on cereal growth. Biologia 69 (3), 281-292.
• Khan, A.L., Hamayun, M., Kang, S.M., Kim, Y.H., Jung, H.Y., Lee, J.H., Lee, I.J. (2012). Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10. BMC Microbiology, 12, 3.
• Lazovic, B., Miranovic, K. (1999). Olive protein content and amino acid composition. Acta Horticulturae, 474, 463–468.
• Mafra, I., Lanza, B., Reis, A., Marsilio, V., Compestre, C., Angelis, M., Coimbro, M.A. (2001). Effect of ripening on texture, microstructure and cell wall polysaccharide composition of olive fruit (Olea europaea). Physiologia Plantarum, 111(4), 439–447.
• Mahadevan, A., Sridhar, R. (1982). Methods in Physiological Plant Pathology. II. Editio., Indra Nagar, Sivakami Publications, Mandras.
• Martin, G.C. (1994). Mechanical olive harvest: using fruit loosening agents. Acta Horticulturae, 356, 284-291.
• Martinez-Toledo, M.V., Moreno, R.J., Gonzalez-Lopez, J. (1988). Root exudates of Zea mays and production of auxins, gibberellins and cytokinins by Azotobacter chroococcum. Plant and Soil, 110(1), 149–152.
• Morales-Sillero, A., García, J.M. (2015). Impact assessment of mechanical harvest on fruit physiology and consequences on oil physicochemical and sensory quality from “Manzanilla de Sevilla” and “Manzanilla Cacereña” super-high-density hedgerows. A preliminary study. Journal of the Science of Food and Agriculture, 95(12), 2445–2453.
• Ozay, G., Borcaklı, M. (1995). Effect of brine replacement and salt concentration on the fermentation of naturally black olives. Food Research International, 28(6), 553–559.
• Ozguven, A.I., Ozguven, F., Tatli, F. (1998). The effects of Ethrel on the ripening and harvesting of olive. Acta Horticulturae, 463, 359–363. Palavan-Unsal, N. (1993). Plant growth regulators. İstanbul University Press and Film Center, İstanbul, Turkey.
• SAS Institute (2011). The SAS system for Windows. Release 9.2. SAS Inst., Cary, NC.
• Unyayar S, (2000) Production of gibberellic acid and cytokinin by Phanerochaete chrysosporium ME446 immobilized on polyurethane foam. Turkish Journal of Biology, 24, 513–519.
• Vierhuis, E., Schols, H.A., Beldman, G., Voragen, A.G.J. (2000). Isolation and characterization of cell wall material from olive fruit (Olea europaea cv koroneiki) at different ripening stages. Carbohydrate Polymers, 43(1), 11–21.
• Vierhuis, E., Korver, M., Schols, H.A., Voragen, G.J. (2003). Structural characteristics of pectic polysaccharides from olive fruit (Olea europaea cv moraiolo) in relation to processing for oil extraction. Carbohydrate Polymers, 51(2), 135–148.
• Yalcinkaya, Y. (1993). The effect of various physiological conditions on the production of indole-3-acetic acid (IAA) of Gibberella fujikuroi G5. MSc Thesis, Hacettepe University, Institute of Science, Ankara, Turkey.