Year 2021, Volume 5 , Issue 1, Pages 1 - 6 2021-03-31

Estimation of total tannin and total phenolic content in plant (Crataegus azarolus L) by orbital shaker technique

Holem Hashm BALAKY [1] , Karzan KHALİD [2] , Avan HASAN [3] , Shadya TAHİR [4] , Sedat UBUR [5] , Ali KHEDİR [6]


The plant of Crataegus Azarolus L, genus that belongs to the Rosaceae family and is a low, dense, spiny tree with a beautiful inflorescence, the phytochemical investigations on genus Crataegus were mainly performed on the leaves, flowers and berries. The objective of this experiment was to determine the ratio of tannin and phenol compound by method orbital shaker extraction. The plant of Crataegus azarolus L was collected in hasanbag mountain soran, Kurdistan. The parts of seed, leaf and steam of Crataegus Azarolus L was extract with water solvent and orbital shaker and assessed yield extraction, total tannin and total phenol. The highest significant value (p<0.05) of yield extraction was observed from leaf (71.56%), compared with other part of Crataegus Azarolus L. On the other hand, the total tannin was showed uppermost in leaf (3.03 kg/mg), and the steam (2.87 kg/mg). Whereas, the smallest total tannin was observed from the seed (1.10%). Furthermore, the total phenolic contents of the plant were observed the lowest value in seed as (0.98 mg GAE/g). Whereas, the total phenol in leaf was observed a higher significantly value (3.68 mg GAE/g) and in steam (1.92 mg GAE/g), with all significant value (p<0.05). This study showed that the leaf and other part of Crataegus Azarolus L, rich in total phenolic and total tannin after orbital shaker method was used.
Crataegus Azarolus, Tannin, Orbital shaker, Total Phenolic, Leaf
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Primary Language en
Subjects Food Science and Technology, Plant Science
Published Date March 2021
Journal Section Research Articles
Authors

Orcid: 0000-0003-0579-9029
Author: Holem Hashm BALAKY (Primary Author)
Institution: General Science Department, Faculty of Education, Soran University, Erbil, Iraq
Country: Iraq


Orcid: 0000-0003-0596-5357
Author: Karzan KHALİD
Institution: Biology department/ Faculty of science ,Soran University, Erbil, Iraq 1
Country: Turkey


Orcid: 0000-0001-6501-8031
Author: Avan HASAN
Institution: General Science Department, Faculty of Education, Soran University, Erbil, Iraq
Country: Iraq


Orcid: 0000-0002-8005-9357
Author: Shadya TAHİR
Institution: General Science Department, Faculty of Education, Soran University, Erbil, Iraq
Country: Turkey


Orcid: 0000-0003-1545-4147
Author: Sedat UBUR
Institution: Kahramanmaras Sutcu Imam University, Forest Faculty, Forest Industry Engineering, Kahramanmaras-Turkey
Country: Turkey


Orcid: 0000-0003-2173-9333
Author: Ali KHEDİR
Institution: 6College of Agriculture Engineering Sciences, University of Salahaddin, Erbil-Iraq
Country: Turkey


Dates

Application Date : August 22, 2020
Acceptance Date : January 11, 2021
Publication Date : March 31, 2021

APA Balaky, H , Khalid, K , Hasan, A , Tahir, S , Ubur, S , Khedir, A . (2021). Estimation of total tannin and total phenolic content in plant (Crataegus azarolus L) by orbital shaker technique . International Journal of Agriculture Environment and Food Sciences , 5 (1) , 1-6 . DOI: 10.31015/jaefs.2021.1.1