This study aimed to isolate and identify acidophilic actinobacteria. Acidophilic actinobacteria isolates were had from a paddy field soil in Osmancık placed near Çorum province in Turkey. The dilution plate technique on seven selective media with pH 5.5 was used for isolation. 16S rRNA gene PCR amplification of acidophilic actinobacteria was performed. Three different algorithms were used in the phylogenetic analyzes made with MEGA 7.0 software. Twenty-two isolates were obtained from seven selective media, and according to 16S rRNA gene sequence analysis of 22 isolates, twenty-one Streptomyces isolates and one Rhodococcus isolate were identified. The antifungal activities of isolated acidophilic actinobacteria against Fusarium moniliforme and Rhizoctonia solani, the rice pathogenic fungi were evaluated. The isolates with antifungal activity have the potential to be used as biological control agents against rice pathogens.