Research Article
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Effects of exogenous glycine betaine application on some physiological and biochemical properties of cotton (G. hirsutum L.) plants grown in different drought levels

Year 2021, Volume: 5 Issue: 4, 689 - 700, 15.12.2021
https://doi.org/10.31015/jaefs.2021.4.30

Abstract

Drought stress significantly reduces the growth and yield of cotton plants; therefore, reducing the damage caused by drought stress and improving the plant growth are highly important. The aims of this study were to investigate some of physiological and biochemical properties of cotton plants exposed to different drought levels and to determine the extent of improvement obtained by exogenous glycine betaine treatment for the damages caused by the adverse effects of drought. Three drought levels were created using PEG (Polyethylene glycol 6000) solution (0%, 10% and 20%). The drought groups were also divided into 2 subgroups by using 0 (control: glycine betaine solution was not applied) and 2 mM glycine betaine solution. The pot experiment was established in a growth chamber with 3 replications and the experiment repeated twice. The changes in the contents of contents of chlorophyll a and b, carotenoid, total dissolved protein, total dissolved carbohydrate and some nutrients were investigated. Chlorophyll a and b, calcium, magnesium, sodium and phosphorus concentrations were decreased, while carotenoid, total dissolved protein and carbohydrate, potassium contents increased with the increasing drought level. The results revealed that adverse effects of drought stress on cotton plants were alleviated by exogenous application of glycine betaine.

Supporting Institution

HÜBAK

Project Number

18204

References

  • Agboma, M., Jones M. G. K., Peltonen-Sainio, P., Rita, H. et al. (1997a). Exogenous glycine betaine enhances grain yield of maize, sorghum and wheat grown under two supplementary watering regimes. Journal of Agronomy and Crop Science, 178(1): 29– 37.
  • Agboma, P., Peltonen-Sainio, P., Hinkkanen, R., Pehu, E. (1997b). Effect of foliar application of glycine betaine on yield components of drought stressed tobacco plants. Experimental Agriculture, 33(3): 345–352.
  • Ali, S., Chaudhary, A., Rizwan, M., Anwar, H.T. et al. (2015). Alleviation of chromium toxicity by glycine betaine is related to elevated antioxidant enzymes and suppressed chromium uptake and oxidative stress in wheat (Triticum aestivum L.). Environmental Science and Pollution Research, 22, 10669–10678.
  • Allakhverdieva, M. Y., Mamedov, D.M., Gasanov, R. A. (2001). The effect of glycine betaine on the heat stability of photosynthetic reactions in thylakoid membranes. Turkish Journal Botany, 25(2001): 11–17.
  • Anju, S., Thakur, P. S., Duvivedi, M. P. (1994). Rapid evaluation of apple varieties for drought. Indian Journal of Horticulture, 51:2, 16-21.
  • UPK, (2019). Total textile and raw material sector export performance report for December 2018. https://www.ithib.org.tr/tr/bilgi-merkezi-raporlar-aylık-ihracat-değerlendirme-bilgi-notları-2018.html. Date of access: 22.10.2019
  • GTB. (2020). T.C. Ministry of Trade, General Directorate of Tradesmen, Craftsmen and Cooperatives, 1-40.
  • Aranjuelo, I., Molero, G., Erice, G., Avice, J. C. et al. (2010). Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.) Journal of Experimental Botany, 62(1):111-123.
  • Baker, N. R. (1994). Chilling stress and photosynthesis. In: Foyer, C.H., Mullineaux, P.M., eds. Causes of photo oxidative stress and amelioration of defense systems in plants (Suppl. 5), Florida, ABD: CRC Press, pp. 127-154.
  • Bartels, D., Sunkar, R. (2005). Drought and salt tolerance in plants. Critical Review Plant Science, 24(1): 23–58.
  • Başal, H., Aydın, Ü., (2006). Water stress in cotton (Gossypium hirsutum L.). Ege University Journal of Agriculture, 43(3): 101-111.
  • Beringer, H., Trolldenier, G. (1978). Influence of K nutrition on response to environmental stress. Plant Physiology, 261: 115-124.
  • Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254.
  • Bota, J., Medrano, H., Flexas, J. (2004). Is photosynthesis limited by decreased Rubisco activity and RuBP content under progressive water stress? New Phytologist, 162(3): 671-681.
  • Bray, E. (1993). Molecular responses to water deficit. Plant Physiology, 103: 1035– 1040.
  • Can, N. (2013). A Physıological Research on The Effects of Drought Stress on Cotton (Gossypium hirsutum L.) Genotypes. Master Thesis, Harran University, Şanlıurfa, Turkey.
  • Cao, F., Liu, L., Ibrahim, W., Cai, Y. et al. (2013). Alleviating effects of exogenous glutathione, glycine betaine, brassinosteroids and salicylic acid on cadmium toxicity in rice seedlings (Oryza sativa). Agrotechnology, 2: 1–6.
  • Chartzoulaki, K., Patakas, A., Kodifis, G., Bosabalidis, A. et al. (2002). Water stress affects leaf anatomy, gas exchange, water relations and growth of two avocado cultivars. Scientia Horticulturae, 95: 39– 50.
  • Chaves, M. M., Flexas, J., Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell, Annals of Botany, 103(4): 551–560.
  • Chen, T.H.H., Murata, N. (2011). Glycine betaine protects plants against abiotic stress: mechanisms and biotechnological applications. Plant, Cell & Environment, 34:1,1-20.
  • Clemens, S. (2001). Molecular mechanisms of plant metal tolerance and homeostasis. Planta, 212: 475–786.
  • Close, T. (1996). Dehydrin: Emergence of a biochemical role of a family of plant dehydration proteins. Physiology Plant, 97: 795–803.
  • Çınar, I. (2003). Carotenoid Pigment Loss of Freeze-dried Plant Samples under Different Storage Conditions, Food Science and Technology/LWT, 37 (3): 363-367.
  • Çırak, C., Esendal, E. (2006). Drought Stress in Soybean. Ondokuz mayıs University Agricultural Faculty Journal, 21: 231-237.
  • Denaxa, N.K., Roussos, P. A, Damvakaris, T., Stournaras, V. (2012). Comparative effects of exogenous glycine betaine, kaolin clay particles and ambiol on photosynthesis, leaf sclerophylly indexes and heat load of olive cv. Chondrolia Chalkidikis under drought. Scientia Horticulturae, 137:87-94.
  • Dietz, K. J., Pfannschmidt, T. (2011). Novel regulators in photosynthetic redox control of plant metabolism and gene expression, Plant Physiology, 155(4): 1477-1485.
  • Dubois, M., Gilles, A., Hamilton, K., Rebers, A. et al. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: 350-356.
  • Flexas, J., Medrano, H. (2002). Drought-inhibition of photosynthesis in C3 plant: stomatal and non-stomatal limitation revisited. Annual Botany, 89: 183-189.
  • Foyer, C. H., Noctor, G. (2009). Redox regulation in photosynthetic organisms: signaling, acclimation, and practical implications, Antioxidant, Redox Signaling, 11(4): 861-905.
  • Guerfel, M., Baccouri, O., Boujnah, D., Chaibi, W. et al. (2009). Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olive (Olea europaea L.) cultivars. Scientia Horticulture, 119: 257–263.
  • Han, B., Kermode, A.R. (1996). Dehydrin-like proteins in castor bean seeds and seedlings are differentially produced in response to ABA and water-deficit-related stresses. Journal of Experimental Botany, 47(7): 933–939.
  • Havlin, L., Beaton, D., Tisdale, L., Nelson, L. (1999). Soil Fertility and Fertilizers: An Introduction to Nutrient Management. Prentice-Hall, Inc., London, 124: 406–425.
  • He, X., Wang, J., Liang, G. (1995). Effects of water stress on photochemical function and protein metabolism of photosystem II in wheat leaves. Physiologia Plantarum, 93: 771-777.
  • Hoagland, D., 1920. Optimum nutrient solutions for plants. Horticulture Science, 52: 562-564.
  • Iqbal, N., Ashraf, M.Y., Ashraf, M. (2005). Influence of water stress and exogenous glisin betain on sunflower achene weight and oil percentage. International Journal of Environmental Science& Technology, 2(2): 155-160.
  • Iqbal, N., Ashraf, M., Ashraf, M.Y. (2009). Influence of exogenous glycine betaine on gas exchange and biomass production in sunflower (Helianthus annuus L,) under water limited conditions. Journal of Agronomy and Crop Science, 195: 420–426.
  • Islam, M.M., Hoque, M.A., Okuma, E., Nasrin, M. et al. (2009). Exogenous proline and glycine betaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells. Journal Plant Physiology, 166: 1587–1597
  • Jenne, E., Rhoades, H., Yien, C., Howe, O. (1958). Change in nutrient element accumulation by corn with depletion of soil moisture. Agronomy Journal, 50: 71–80.
  • Jin, P., Zhang, Y., Shan, T., Huang, Y. et al. (2015). Low Temperature Conditioning Alleviates Chilling Injury in Loquat Fruit and Regulates Glycine Betaine Content and Energy Status, Agricultural and Food Chemistry, 63: 3654-3659.
  • Kalafetoğlu, T., Ekmekçi, Y. (2005). The effect of drought on plants and tolerance mechanisms. Journal of Science, 18: 723-740
  • Karabudak, T., Bor, M., Özdemir, F. (2014). Glycine Betaine Protects Tomato (Solanum lycopersicum) Plants at Low Temperature By inducing Fatty Acid Desaturase7 and Lipoxygenase Gene Expression. Molecular Biology Reports, 41: 1401-1410.
  • Kerepesi, I., Galiba, G. (2000). Osmotic and Salt Stress-Induced Alteration in Soluble Carbohydrate Content in Wheat Seedlings. Crop science, 40: 482487.
  • Kim, J., Mahe, A., Brangeon, J., Prioul, J. (2000). A maize vacuolar invertase, IVR2, is induced by water stress. Organ/tissue specificity and diurnal modulation of expression. Plant Physiology, 124: 71–84.
  • Lawlor, D. W., Tezara, W. (2009). Causes of decreased photosynthetic rate and metabolic capacity in water-deficient leaf cells: a critical evaluation of mechanisms and integration of processes. Annals of Botany, 103(4): 561–579. Lichtenthaler, H. K. (1987). Pigments of photosynthetic biomembranes and carotenoids: Methods in enzymology 148: 350-382.
  • Loka, D. A., Oosterhuis, D. M. (2014). Water-deficit stress effects on pistil biochemistry and leaf physiology in cotton (Gossypium hirsutum, L.). South African Journal of Botany, 93: 131-136.
  • Marschner H (1995). Mineral nutrition of higher plants. 2nd edn. Academic Press, New York, pp. 889.
  • Mc Neil, S. D., Nuccio, M. L., Andrew, D. H. (1999). Betaines and Related Osmoprotectants. Targets for Metabolic Engineering of Stress Resistance. Plant Physiology, 120: 945-949.
  • Morgan, J. (1992). Osmotic components and properties associated with genotypic differences in osmoregulation in wheat. Australian Journal Plant Physiology, 19: 67–76.
Year 2021, Volume: 5 Issue: 4, 689 - 700, 15.12.2021
https://doi.org/10.31015/jaefs.2021.4.30

Abstract

Project Number

18204

References

  • Agboma, M., Jones M. G. K., Peltonen-Sainio, P., Rita, H. et al. (1997a). Exogenous glycine betaine enhances grain yield of maize, sorghum and wheat grown under two supplementary watering regimes. Journal of Agronomy and Crop Science, 178(1): 29– 37.
  • Agboma, P., Peltonen-Sainio, P., Hinkkanen, R., Pehu, E. (1997b). Effect of foliar application of glycine betaine on yield components of drought stressed tobacco plants. Experimental Agriculture, 33(3): 345–352.
  • Ali, S., Chaudhary, A., Rizwan, M., Anwar, H.T. et al. (2015). Alleviation of chromium toxicity by glycine betaine is related to elevated antioxidant enzymes and suppressed chromium uptake and oxidative stress in wheat (Triticum aestivum L.). Environmental Science and Pollution Research, 22, 10669–10678.
  • Allakhverdieva, M. Y., Mamedov, D.M., Gasanov, R. A. (2001). The effect of glycine betaine on the heat stability of photosynthetic reactions in thylakoid membranes. Turkish Journal Botany, 25(2001): 11–17.
  • Anju, S., Thakur, P. S., Duvivedi, M. P. (1994). Rapid evaluation of apple varieties for drought. Indian Journal of Horticulture, 51:2, 16-21.
  • UPK, (2019). Total textile and raw material sector export performance report for December 2018. https://www.ithib.org.tr/tr/bilgi-merkezi-raporlar-aylık-ihracat-değerlendirme-bilgi-notları-2018.html. Date of access: 22.10.2019
  • GTB. (2020). T.C. Ministry of Trade, General Directorate of Tradesmen, Craftsmen and Cooperatives, 1-40.
  • Aranjuelo, I., Molero, G., Erice, G., Avice, J. C. et al. (2010). Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.) Journal of Experimental Botany, 62(1):111-123.
  • Baker, N. R. (1994). Chilling stress and photosynthesis. In: Foyer, C.H., Mullineaux, P.M., eds. Causes of photo oxidative stress and amelioration of defense systems in plants (Suppl. 5), Florida, ABD: CRC Press, pp. 127-154.
  • Bartels, D., Sunkar, R. (2005). Drought and salt tolerance in plants. Critical Review Plant Science, 24(1): 23–58.
  • Başal, H., Aydın, Ü., (2006). Water stress in cotton (Gossypium hirsutum L.). Ege University Journal of Agriculture, 43(3): 101-111.
  • Beringer, H., Trolldenier, G. (1978). Influence of K nutrition on response to environmental stress. Plant Physiology, 261: 115-124.
  • Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254.
  • Bota, J., Medrano, H., Flexas, J. (2004). Is photosynthesis limited by decreased Rubisco activity and RuBP content under progressive water stress? New Phytologist, 162(3): 671-681.
  • Bray, E. (1993). Molecular responses to water deficit. Plant Physiology, 103: 1035– 1040.
  • Can, N. (2013). A Physıological Research on The Effects of Drought Stress on Cotton (Gossypium hirsutum L.) Genotypes. Master Thesis, Harran University, Şanlıurfa, Turkey.
  • Cao, F., Liu, L., Ibrahim, W., Cai, Y. et al. (2013). Alleviating effects of exogenous glutathione, glycine betaine, brassinosteroids and salicylic acid on cadmium toxicity in rice seedlings (Oryza sativa). Agrotechnology, 2: 1–6.
  • Chartzoulaki, K., Patakas, A., Kodifis, G., Bosabalidis, A. et al. (2002). Water stress affects leaf anatomy, gas exchange, water relations and growth of two avocado cultivars. Scientia Horticulturae, 95: 39– 50.
  • Chaves, M. M., Flexas, J., Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell, Annals of Botany, 103(4): 551–560.
  • Chen, T.H.H., Murata, N. (2011). Glycine betaine protects plants against abiotic stress: mechanisms and biotechnological applications. Plant, Cell & Environment, 34:1,1-20.
  • Clemens, S. (2001). Molecular mechanisms of plant metal tolerance and homeostasis. Planta, 212: 475–786.
  • Close, T. (1996). Dehydrin: Emergence of a biochemical role of a family of plant dehydration proteins. Physiology Plant, 97: 795–803.
  • Çınar, I. (2003). Carotenoid Pigment Loss of Freeze-dried Plant Samples under Different Storage Conditions, Food Science and Technology/LWT, 37 (3): 363-367.
  • Çırak, C., Esendal, E. (2006). Drought Stress in Soybean. Ondokuz mayıs University Agricultural Faculty Journal, 21: 231-237.
  • Denaxa, N.K., Roussos, P. A, Damvakaris, T., Stournaras, V. (2012). Comparative effects of exogenous glycine betaine, kaolin clay particles and ambiol on photosynthesis, leaf sclerophylly indexes and heat load of olive cv. Chondrolia Chalkidikis under drought. Scientia Horticulturae, 137:87-94.
  • Dietz, K. J., Pfannschmidt, T. (2011). Novel regulators in photosynthetic redox control of plant metabolism and gene expression, Plant Physiology, 155(4): 1477-1485.
  • Dubois, M., Gilles, A., Hamilton, K., Rebers, A. et al. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: 350-356.
  • Flexas, J., Medrano, H. (2002). Drought-inhibition of photosynthesis in C3 plant: stomatal and non-stomatal limitation revisited. Annual Botany, 89: 183-189.
  • Foyer, C. H., Noctor, G. (2009). Redox regulation in photosynthetic organisms: signaling, acclimation, and practical implications, Antioxidant, Redox Signaling, 11(4): 861-905.
  • Guerfel, M., Baccouri, O., Boujnah, D., Chaibi, W. et al. (2009). Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olive (Olea europaea L.) cultivars. Scientia Horticulture, 119: 257–263.
  • Han, B., Kermode, A.R. (1996). Dehydrin-like proteins in castor bean seeds and seedlings are differentially produced in response to ABA and water-deficit-related stresses. Journal of Experimental Botany, 47(7): 933–939.
  • Havlin, L., Beaton, D., Tisdale, L., Nelson, L. (1999). Soil Fertility and Fertilizers: An Introduction to Nutrient Management. Prentice-Hall, Inc., London, 124: 406–425.
  • He, X., Wang, J., Liang, G. (1995). Effects of water stress on photochemical function and protein metabolism of photosystem II in wheat leaves. Physiologia Plantarum, 93: 771-777.
  • Hoagland, D., 1920. Optimum nutrient solutions for plants. Horticulture Science, 52: 562-564.
  • Iqbal, N., Ashraf, M.Y., Ashraf, M. (2005). Influence of water stress and exogenous glisin betain on sunflower achene weight and oil percentage. International Journal of Environmental Science& Technology, 2(2): 155-160.
  • Iqbal, N., Ashraf, M., Ashraf, M.Y. (2009). Influence of exogenous glycine betaine on gas exchange and biomass production in sunflower (Helianthus annuus L,) under water limited conditions. Journal of Agronomy and Crop Science, 195: 420–426.
  • Islam, M.M., Hoque, M.A., Okuma, E., Nasrin, M. et al. (2009). Exogenous proline and glycine betaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells. Journal Plant Physiology, 166: 1587–1597
  • Jenne, E., Rhoades, H., Yien, C., Howe, O. (1958). Change in nutrient element accumulation by corn with depletion of soil moisture. Agronomy Journal, 50: 71–80.
  • Jin, P., Zhang, Y., Shan, T., Huang, Y. et al. (2015). Low Temperature Conditioning Alleviates Chilling Injury in Loquat Fruit and Regulates Glycine Betaine Content and Energy Status, Agricultural and Food Chemistry, 63: 3654-3659.
  • Kalafetoğlu, T., Ekmekçi, Y. (2005). The effect of drought on plants and tolerance mechanisms. Journal of Science, 18: 723-740
  • Karabudak, T., Bor, M., Özdemir, F. (2014). Glycine Betaine Protects Tomato (Solanum lycopersicum) Plants at Low Temperature By inducing Fatty Acid Desaturase7 and Lipoxygenase Gene Expression. Molecular Biology Reports, 41: 1401-1410.
  • Kerepesi, I., Galiba, G. (2000). Osmotic and Salt Stress-Induced Alteration in Soluble Carbohydrate Content in Wheat Seedlings. Crop science, 40: 482487.
  • Kim, J., Mahe, A., Brangeon, J., Prioul, J. (2000). A maize vacuolar invertase, IVR2, is induced by water stress. Organ/tissue specificity and diurnal modulation of expression. Plant Physiology, 124: 71–84.
  • Lawlor, D. W., Tezara, W. (2009). Causes of decreased photosynthetic rate and metabolic capacity in water-deficient leaf cells: a critical evaluation of mechanisms and integration of processes. Annals of Botany, 103(4): 561–579. Lichtenthaler, H. K. (1987). Pigments of photosynthetic biomembranes and carotenoids: Methods in enzymology 148: 350-382.
  • Loka, D. A., Oosterhuis, D. M. (2014). Water-deficit stress effects on pistil biochemistry and leaf physiology in cotton (Gossypium hirsutum, L.). South African Journal of Botany, 93: 131-136.
  • Marschner H (1995). Mineral nutrition of higher plants. 2nd edn. Academic Press, New York, pp. 889.
  • Mc Neil, S. D., Nuccio, M. L., Andrew, D. H. (1999). Betaines and Related Osmoprotectants. Targets for Metabolic Engineering of Stress Resistance. Plant Physiology, 120: 945-949.
  • Morgan, J. (1992). Osmotic components and properties associated with genotypic differences in osmoregulation in wheat. Australian Journal Plant Physiology, 19: 67–76.
There are 48 citations in total.

Details

Primary Language English
Subjects Agronomy
Journal Section Research Articles
Authors

Nimet Kılınçoğlu 0000-0001-7935-9216

Cevher İlhan Cevheri 0000-0002-7070-2652

Cenap Cevheri 0000-0002-3759-4645

Hatice Yüsra Nahya 0000-0003-2228-5118

Project Number 18204
Publication Date December 15, 2021
Submission Date September 16, 2021
Acceptance Date December 20, 2021
Published in Issue Year 2021 Volume: 5 Issue: 4

Cite

APA Kılınçoğlu, N., Cevheri, C. İ., Cevheri, C., Nahya, H. Y. (2021). Effects of exogenous glycine betaine application on some physiological and biochemical properties of cotton (G. hirsutum L.) plants grown in different drought levels. International Journal of Agriculture Environment and Food Sciences, 5(4), 689-700. https://doi.org/10.31015/jaefs.2021.4.30


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