Research Article
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Immunomodulation function of Tunceli garlic (Allium tuncelianum) oil in Rainbow Trout (Oncorhynchus mykiss)

Year 2022, Volume 6, Issue 1, 7 - 12, 15.03.2022
https://doi.org/10.31015/jaefs.2022.1.2

Abstract

The effects of injectable Tunceli garlic oil (Allium tuncelianum) on serum response of rainbow trout were examined. Influence of intraperitoneal (i.p.) injection administration of Tunceli garlic oil (TGO) was evaluated on some immunological factors of rainbow trout. Fish weight 40 g were i.p. injected TGO at doses of 1% and 10%. Serum lysozyme activity, bactericidal activity, myeloperoxidase, protein and immunoglobulin levels were measured on days 3, 7, 14 and 21 after the TGO administration. All the immune parameters assayed remained affected when TGO was i.p. administered. Serum bactericidal activity was consistently achieved by immunizing fish with TGO. Levels of serum myeloperoxidase activity were increased in the groups of fish injected with TGO. Myeloperoxidase and bactericidal activities peaked at 14 and 21 days post-injection, respectively. Lysozyme activity was significantly increased throughout experiment in the all groups. Total protein and immunoglobulin levels increased in the 21 days post-injection although no statistically significant differences. In conclusion, these results suggest that there are functionally effects of TGO on the immune mechanisms of rainbow trout.

References

  • Abd El-Galil, M.A.A. and Aboelhadid, S.M. (2012). Trials for the control of trichodinosis and gyrodactylosis in hatchery reared Oreochromis niloticus fries by using garlic, Veterinary Parasitology, 30;185 (2-4): 57-63. Doi: https://doi.org/10.1016/j.vetpar.2011.10.035
  • Abdelhamid, A.M., Khalil, F. F., El-Barbery, M.I., Zaki V.H. and Husien, H.S. (2002). Feeding Nile tilapia on biogen to detoxify aflatoxin diet. In: Annual scientific conference of animal and fish production, 1, Mansoura. Proceedings. Mansoura University, pp. 207-230.
  • Baktır, I. (2005). Tunceli Sarımsağı’nın (Allium tuncelianum) in vitro koşullarında çoğaltılması. In: Proceeding of GAP IV. Tarım Kongresi (in Turkish).
  • Colorni, A., Avtalion, R., Knibb, W., Berger, E., Colorni, B. and Timan, B. (1998). Histopathology of sea bass Dicentrarchus labrax experimentally infected with Mycobacterium marinum and treated with streptomycin and garlic (Allium sativum) extract. Aquac. 190: 1-17. Doi: https://doi.org/10.1016/S0044-8486(97)00234-2
  • Deresse, D. (2010). Antibacterial effect of garlic (Allium sativum) on Staphylococcus aureus: An in vitro study, Asian J. Med. Sci. 2 (2): 62–5.
  • Diab, A.S., Aly, S. M., John, G., Abde-Hadi, Y. and Mohammed, M.F. (2008). Effect of garlic, black seed and bioiogen as immunostimulants on the growth and survival of Nile tilapia, Oreochromis niloticus (Teleostei: Cichlidae), and their response to artificial infection with Pseudomonas fluorescens. Afr. J. Aquat. Sci. 33 (1): 63-68. Doi: https://doi.org/10.2989/AJAS.2007.33.1.7.391
  • Etoh, T. and Simon, P. W. (2002). Diversity, fertility and seed production of garlic. In H. D. Rabinowitch, L. Currah (Eds.), Allium Crop Science: Recent Advances. CABI Publishing, New York. pp. 101–117.
  • Farahi, A., Kasiri, M., Sudagar, M., Iraei, M. S. and Shahkolaei, M.D. (2010). Effect of garlic (Allium sativum) on growth factors, some hematological parameters and body compositions in rainbow trout (Oncorhynchus mykiss). Aquaculture, Aquarium, Conservation and Legislation International Journal of the Bioflux Society, 3 (4): 317-323.
  • Guo, J.J., Kuo, C.M., Chuang, Y.C., Hong, J.W., Chou, R.L. and Chen, T.I. (2012). The effects of garlic-supplemented diets on antibacterial activity against Streptococcus iniae and on growth in orange-spotted grouper, Epinephelus coioides, Aquaculture, 33 (38): 364–365. Doi: https://doi.org/10.1016/j.aquaculture.2012.07.023
  • Ipek, M., Ipek, A. and Simon, P.W. (2008). Genetic characterization of Allium tuncelianum: An endemic edible Allium species with garlic odor. Sci Hortic. 115, 409–415. Doi: https://doi.org/10.1016/j.scienta.2007.11.002
  • Ispir, U. and Dorucu, M. (2005). A study on the effects of levamisole of immune system Rainbow trout (Oncorhynchus mykiss, Walbaum). Turk J. Vet. Anim. Sci. 29: 1169-1176.
  • Ispir, U., Gokhan, H.B., Ozcan, M., Dorucu, M. and Saglam, N. (2009). Immune response of rainbow trout (Oncorhynchus mykiss) to selected antigens of Yersinia ruckeri. Acta Veterinaria Brno, 78: 145-150. Doi: Doi: https://doi.org/10.2754/avb200978010145
  • Khattab, Y.A., Shalaby, A.M.E., Sharaf, S.M., El-Marakby, H.I. and Rizkalla, E.H. (2004). The physiological changes and growth performance of the Nile tilapia Oreochromis niloticus after feeding with Biogen as growth promoter. Egypt. J. Aquat. Biol and Fish. 8: 145-158.
  • Nya, E.J. and Austin, B. (2009). Use of garlic, Allium sativum, to control Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish. Dis. 32 (11): 963–970. Doi: https://doi.org/10.1111/j.1365-2761.2009.01100.x
  • Nya, E.J. and Austin, B. (2011). Development of immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) to Aeromonas hydrophila after the dietary application of garlic. Fish Shellfish Immunol. 30 (3): 845-850. Doi: https://doi.org/10.1016/j.fsi.2011.01.008
  • Punitha, S.M.J., Babu, M.M., Sivaram, V., Shankar, V.S., Dhas, S.A., Mahesh, T.C., Immanuel, G. and Citarasu, T. (2008). Immunostimulating influence of herbal biomedicines on nonspecific immunity in Grouper Epinephelus tauvina juvenile against Vibrio harveyi infection, Aquaculture Int. 16: 511–523. Doi: https://doi.org/10.1007/s10499-007-9162-6
  • Quade, M.J. and Roth, J. A. (1997). A rapid, direct assay to measure degranulation of bovine neutrophil primary granules. Vet Immunol Immunop, 58: 239-248. Doi: https://doi.org/10.1016/S0165-2427(97)00048-2
  • Ranjan, S., Dasgupta, N., Saha, P., Rakshit, M. and Ramalingam, C. (2012). Comparative study of antibacterial activity of garlic and cinnamon at different temperature and its application on preservation of fish, Adv. Appl. Sci. Res. 3(1):495-501.
  • Sahoo, P. K., Kumari, J. and Mishra, B. K. (2005). Non-specific immune responses in juveniles of Indian major carps, J. Appl. Ichthyol. 21: 151-155. Doi: https://doi.org/10.1111/j.1439-0426.2004.00606.x
  • Sahu, S., Das, B. K., Mishra, B. K., Pradhan, J. and Sarangi, N. (2007). Effect of Allium sativum on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. J. Appl. Ichthyol. 23(1):80–6. Doi: https://doi.org/10.1111/j.1439-0426.2006.00785.x
  • Shalaby, A.M., Khattab, Y.A. and Abdel Rahman, A.M. (2006). Effect of garlic (Allium sativum) and chloramphenicol on groth performance, physiological parameters and survival of Nile tilapia (Oreochromis niloticus). J. Venom Anim. Toxins incl. Trop. Dis. 12 (2):172-201. Doi: https://doi.org/10.1590/S1678-91992006000200003
  • Siwicki, A.K., Anderson, D.P. and Rumsey, G.L. (1994). Dietary intake of immunostimulants by rainbow trout effects non-specific immunity and protection against furuncluosis. Vet. Immunol. Immunopathol. 41: 125-139. Doi: https://doi.org/10.1016/0165-2427(94)90062-0
  • Tafalla, C., Novoa, B., Alvarez, J.M. and Figueras, A. (1999). In vivo and in vitro effect of oxytetracycline treatment on the immune response of turbot, Scophthalmus maximus. Journal of Fish Diseases, 22, 271–276. Doi: https://doi.org/10.1046/j.1365-2761.1999.00179.x
  • Talpur, A.D. and Ikhwanuddin, M. (2012). Dietary effects of garlic (Allium sativum) on haemato immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian sea bass, Lates calcarifer (Bloch). Aquac. 364-365:6-12. Doi: https://doi.org/10.1016/j.aquaculture.2012.07.035
  • Thanikachalam, K., Kasi, M. and Rathinam, X. (2010). Effect of garlic peel on growth, hematological parameters and disease resistance against Aeromonas hydrophila in African catfish Clarias gariepinus (Bloch) fingerlings. Asian Pacific Journal of Tropical Medicine, 614-618. Doi: https://doi.org/10.1016/S1995-7645(10)60149-6
  • Williams, C. F., Lloyda, D., Kolarich, D., Alagesan, K., Duchêne, M., Cablea, J., Williams, D. and Leitsch, D. (2012). Disrupted intracellular redox balance of the diplomonad fish parasite Spironucleus vortens by 5-nitroimidazoles and garlic-derived compounds. Veterinary Parasitology, 190(1–2), 62-73. Doi: https://doi.org/10.1016/j.vetpar.2012.05.011
  • Wu, Y.R., Gong, Q.F., Fang, H., Liang, W.W., Chen, M. and He, R.J. (2013). Effect of Sophora flavescens on non-specific immune response of tilapia (GIFT Oreochromis niloticus) and disease resistance against Streptococcus agalactiae. Fish. Shellfish Immunol. 34: 220-227. Doi: https://doi.org/10.1016/j.fsi.2012.10.020
  • Yonar, M.E. (2002). Sülfamerazinin Gökkuşağı alabalığının immun sistemi üzerine etkisinin araştırılması. Fırat Üniversitesi Fen Bilimleri Enstitüsü Su Ürünleri Yetiştiriciliği Anabilim Dalı. Yüksek Lisans Tezi (in Turkish).
  • Zhang, J., Zou, W. and Yan, Q. (2008). Non-specific immune response of bullfrog Rana catesbeiana to intraperitoneal injection of bacterium Aeromonas hydrophila. Chinese Journal of Oceanology and Limnology, 26, 248–255. Doi: https://doi.org/1007/s00343-008-0248-4

Year 2022, Volume 6, Issue 1, 7 - 12, 15.03.2022
https://doi.org/10.31015/jaefs.2022.1.2

Abstract

References

  • Abd El-Galil, M.A.A. and Aboelhadid, S.M. (2012). Trials for the control of trichodinosis and gyrodactylosis in hatchery reared Oreochromis niloticus fries by using garlic, Veterinary Parasitology, 30;185 (2-4): 57-63. Doi: https://doi.org/10.1016/j.vetpar.2011.10.035
  • Abdelhamid, A.M., Khalil, F. F., El-Barbery, M.I., Zaki V.H. and Husien, H.S. (2002). Feeding Nile tilapia on biogen to detoxify aflatoxin diet. In: Annual scientific conference of animal and fish production, 1, Mansoura. Proceedings. Mansoura University, pp. 207-230.
  • Baktır, I. (2005). Tunceli Sarımsağı’nın (Allium tuncelianum) in vitro koşullarında çoğaltılması. In: Proceeding of GAP IV. Tarım Kongresi (in Turkish).
  • Colorni, A., Avtalion, R., Knibb, W., Berger, E., Colorni, B. and Timan, B. (1998). Histopathology of sea bass Dicentrarchus labrax experimentally infected with Mycobacterium marinum and treated with streptomycin and garlic (Allium sativum) extract. Aquac. 190: 1-17. Doi: https://doi.org/10.1016/S0044-8486(97)00234-2
  • Deresse, D. (2010). Antibacterial effect of garlic (Allium sativum) on Staphylococcus aureus: An in vitro study, Asian J. Med. Sci. 2 (2): 62–5.
  • Diab, A.S., Aly, S. M., John, G., Abde-Hadi, Y. and Mohammed, M.F. (2008). Effect of garlic, black seed and bioiogen as immunostimulants on the growth and survival of Nile tilapia, Oreochromis niloticus (Teleostei: Cichlidae), and their response to artificial infection with Pseudomonas fluorescens. Afr. J. Aquat. Sci. 33 (1): 63-68. Doi: https://doi.org/10.2989/AJAS.2007.33.1.7.391
  • Etoh, T. and Simon, P. W. (2002). Diversity, fertility and seed production of garlic. In H. D. Rabinowitch, L. Currah (Eds.), Allium Crop Science: Recent Advances. CABI Publishing, New York. pp. 101–117.
  • Farahi, A., Kasiri, M., Sudagar, M., Iraei, M. S. and Shahkolaei, M.D. (2010). Effect of garlic (Allium sativum) on growth factors, some hematological parameters and body compositions in rainbow trout (Oncorhynchus mykiss). Aquaculture, Aquarium, Conservation and Legislation International Journal of the Bioflux Society, 3 (4): 317-323.
  • Guo, J.J., Kuo, C.M., Chuang, Y.C., Hong, J.W., Chou, R.L. and Chen, T.I. (2012). The effects of garlic-supplemented diets on antibacterial activity against Streptococcus iniae and on growth in orange-spotted grouper, Epinephelus coioides, Aquaculture, 33 (38): 364–365. Doi: https://doi.org/10.1016/j.aquaculture.2012.07.023
  • Ipek, M., Ipek, A. and Simon, P.W. (2008). Genetic characterization of Allium tuncelianum: An endemic edible Allium species with garlic odor. Sci Hortic. 115, 409–415. Doi: https://doi.org/10.1016/j.scienta.2007.11.002
  • Ispir, U. and Dorucu, M. (2005). A study on the effects of levamisole of immune system Rainbow trout (Oncorhynchus mykiss, Walbaum). Turk J. Vet. Anim. Sci. 29: 1169-1176.
  • Ispir, U., Gokhan, H.B., Ozcan, M., Dorucu, M. and Saglam, N. (2009). Immune response of rainbow trout (Oncorhynchus mykiss) to selected antigens of Yersinia ruckeri. Acta Veterinaria Brno, 78: 145-150. Doi: Doi: https://doi.org/10.2754/avb200978010145
  • Khattab, Y.A., Shalaby, A.M.E., Sharaf, S.M., El-Marakby, H.I. and Rizkalla, E.H. (2004). The physiological changes and growth performance of the Nile tilapia Oreochromis niloticus after feeding with Biogen as growth promoter. Egypt. J. Aquat. Biol and Fish. 8: 145-158.
  • Nya, E.J. and Austin, B. (2009). Use of garlic, Allium sativum, to control Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish. Dis. 32 (11): 963–970. Doi: https://doi.org/10.1111/j.1365-2761.2009.01100.x
  • Nya, E.J. and Austin, B. (2011). Development of immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) to Aeromonas hydrophila after the dietary application of garlic. Fish Shellfish Immunol. 30 (3): 845-850. Doi: https://doi.org/10.1016/j.fsi.2011.01.008
  • Punitha, S.M.J., Babu, M.M., Sivaram, V., Shankar, V.S., Dhas, S.A., Mahesh, T.C., Immanuel, G. and Citarasu, T. (2008). Immunostimulating influence of herbal biomedicines on nonspecific immunity in Grouper Epinephelus tauvina juvenile against Vibrio harveyi infection, Aquaculture Int. 16: 511–523. Doi: https://doi.org/10.1007/s10499-007-9162-6
  • Quade, M.J. and Roth, J. A. (1997). A rapid, direct assay to measure degranulation of bovine neutrophil primary granules. Vet Immunol Immunop, 58: 239-248. Doi: https://doi.org/10.1016/S0165-2427(97)00048-2
  • Ranjan, S., Dasgupta, N., Saha, P., Rakshit, M. and Ramalingam, C. (2012). Comparative study of antibacterial activity of garlic and cinnamon at different temperature and its application on preservation of fish, Adv. Appl. Sci. Res. 3(1):495-501.
  • Sahoo, P. K., Kumari, J. and Mishra, B. K. (2005). Non-specific immune responses in juveniles of Indian major carps, J. Appl. Ichthyol. 21: 151-155. Doi: https://doi.org/10.1111/j.1439-0426.2004.00606.x
  • Sahu, S., Das, B. K., Mishra, B. K., Pradhan, J. and Sarangi, N. (2007). Effect of Allium sativum on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. J. Appl. Ichthyol. 23(1):80–6. Doi: https://doi.org/10.1111/j.1439-0426.2006.00785.x
  • Shalaby, A.M., Khattab, Y.A. and Abdel Rahman, A.M. (2006). Effect of garlic (Allium sativum) and chloramphenicol on groth performance, physiological parameters and survival of Nile tilapia (Oreochromis niloticus). J. Venom Anim. Toxins incl. Trop. Dis. 12 (2):172-201. Doi: https://doi.org/10.1590/S1678-91992006000200003
  • Siwicki, A.K., Anderson, D.P. and Rumsey, G.L. (1994). Dietary intake of immunostimulants by rainbow trout effects non-specific immunity and protection against furuncluosis. Vet. Immunol. Immunopathol. 41: 125-139. Doi: https://doi.org/10.1016/0165-2427(94)90062-0
  • Tafalla, C., Novoa, B., Alvarez, J.M. and Figueras, A. (1999). In vivo and in vitro effect of oxytetracycline treatment on the immune response of turbot, Scophthalmus maximus. Journal of Fish Diseases, 22, 271–276. Doi: https://doi.org/10.1046/j.1365-2761.1999.00179.x
  • Talpur, A.D. and Ikhwanuddin, M. (2012). Dietary effects of garlic (Allium sativum) on haemato immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian sea bass, Lates calcarifer (Bloch). Aquac. 364-365:6-12. Doi: https://doi.org/10.1016/j.aquaculture.2012.07.035
  • Thanikachalam, K., Kasi, M. and Rathinam, X. (2010). Effect of garlic peel on growth, hematological parameters and disease resistance against Aeromonas hydrophila in African catfish Clarias gariepinus (Bloch) fingerlings. Asian Pacific Journal of Tropical Medicine, 614-618. Doi: https://doi.org/10.1016/S1995-7645(10)60149-6
  • Williams, C. F., Lloyda, D., Kolarich, D., Alagesan, K., Duchêne, M., Cablea, J., Williams, D. and Leitsch, D. (2012). Disrupted intracellular redox balance of the diplomonad fish parasite Spironucleus vortens by 5-nitroimidazoles and garlic-derived compounds. Veterinary Parasitology, 190(1–2), 62-73. Doi: https://doi.org/10.1016/j.vetpar.2012.05.011
  • Wu, Y.R., Gong, Q.F., Fang, H., Liang, W.W., Chen, M. and He, R.J. (2013). Effect of Sophora flavescens on non-specific immune response of tilapia (GIFT Oreochromis niloticus) and disease resistance against Streptococcus agalactiae. Fish. Shellfish Immunol. 34: 220-227. Doi: https://doi.org/10.1016/j.fsi.2012.10.020
  • Yonar, M.E. (2002). Sülfamerazinin Gökkuşağı alabalığının immun sistemi üzerine etkisinin araştırılması. Fırat Üniversitesi Fen Bilimleri Enstitüsü Su Ürünleri Yetiştiriciliği Anabilim Dalı. Yüksek Lisans Tezi (in Turkish).
  • Zhang, J., Zou, W. and Yan, Q. (2008). Non-specific immune response of bullfrog Rana catesbeiana to intraperitoneal injection of bacterium Aeromonas hydrophila. Chinese Journal of Oceanology and Limnology, 26, 248–255. Doi: https://doi.org/1007/s00343-008-0248-4

Details

Primary Language English
Subjects Agriculture
Published Date March 2022
Journal Section Research Articles
Authors

Ünal İSPİR
MALATYA TURGUT ÖZAL ÜNİVERSİTESİ, DOĞANŞEHİR VAHAP KÜÇÜK MESLEK YÜKSEKOKULU
0000-0002-2077-0219
Türkiye


Mikail ÖZCAN (Primary Author)
Kahramanmaraş Sütçü İmam Üniversitesi, Ziraat Fakültesi, Su Ürünleri Bölümü
0000-0001-9032-0697
Türkiye


Engin ŞEKER
MUNZUR ÜNİVERSİTESİ, SU ÜRÜNLERİ FAKÜLTESİ
0000-0003-2906-6006
Türkiye

Publication Date March 15, 2022
Application Date July 28, 2021
Acceptance Date January 18, 2022
Published in Issue Year 2022, Volume 6, Issue 1

Cite

APA İspir, Ü. , Özcan, M. & Şeker, E. (2022). Immunomodulation function of Tunceli garlic (Allium tuncelianum) oil in Rainbow Trout (Oncorhynchus mykiss) . International Journal of Agriculture Environment and Food Sciences , 6 (1) , 7-12 . DOI: 10.31015/jaefs.2022.1.2


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