Research Article
BibTex RIS Cite

Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.)

Year 2022, Volume: 6 Issue: 4, 598 - 604, 30.12.2022
https://doi.org/10.31015/jaefs.2022.4.13

Abstract

In this study, an in vitro propagation protocol of Poncirus trifoliata L. (Raf.) rootstock, which is one of the main rootstocks used in citrus, was created. Monocular node parts of the plant, which is the source of explant, were cultured in MS nutrient media containing BAP at dissmilar concentrations (0.5-1.0-2.0 mg/l). Afterward, it was observed that shoot appearance and tillering were achieved in the node explants cultured in MS nutrient medium comprising 1.0 mg/l BAP. The shoots were removed to a new culture in MS nutrient media containing NAA and IBA for rooting. While 30% rooting was achieved in the rooting medium, 62% of the rooted plantlets were acclimated to external conditions. 2 mg/l BAP and 2 mg/l silver nitrate (AgNO3) doses were supplied to the MS medium. The results of this study were obtained and a compound was found to the yellowing and leaf fall problem. As a result, in vitro propagation of Poncirus trifoliata, which is among the important citrus rootstocks, was tried, and its rooting ability was investigated in different nutrient media concentrations after shoots were obtained from different explants.

Supporting Institution

Harran University Scientific Research Projects Coordination Unit (HUBAK)

Project Number

21096

References

  • Ainsley, P.J., Collins, G.G., Sedgley, M. (2001a). In Vitro Rooting of Almond (Prunus dulcis Mill.). In vitro Cellular and Development Biology Plant, 37:778-785.
  • Ainsley, P.J., Hammerschlag, F.A., Bertozzi, T., Collins, G.G., Sedgley, M. (2001b). Regeneration of Almond from Immature Seed Cotyledons. Plant Cell, Tissue and Organ Culture, 67: 221-226.
  • Ali, S., Mirza, B. (2006). Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explanttype and hormone concentration. Acta Botanica Croatica 65(2), 137-146.
  • Ak, B.E., Karahan Kiyar, P., Hatipoglu, I.H., Dikmetas, B. (2021). Effects of Different BA and IBA Concentrations on Proliferation and Rooting of ‘Garnem’ Rootstock In-vitro Propagation. International Journal of Agriculture Environment and Food Sciences, 5(4):470-476, DOI: 10.31015/jaefs.2021.4.6
  • Amgai, R.B., Prasai, H.K., Pandey, Y.R. (2016). Hormonal effect on mandarin orange (Citrusreticulata Blanco) micropropagation. Nepal Journal of Biotechnology, 4(1), 33-36. doi: 10.3126/njb.v4i1.16344
  • Babaoglu, M., Gurel, E. Ozcan, S. (2002). Bitki Biyoteknolojisi. Selçuk Üniversitesi Vakfı Yayınları, 374s.
  • Cengiz, M., Aka Kacar, Y. (2019). Micropropagation of Some Citrus Rootstocks with Classical and New Generation Tissue Culture Techniques. Turkish Journal of Agriculture - Food Science and Technology 7(9):1469.
  • Chamandoosti (2017).International Journal of Environmental&AgricultureResearch (IJOEAR).2454-1850
  • Espinosa, A.C., Pijut, P.M., Michler, C.H. (2006). Adventitious Shoot Regeneration and Rooting of Prunus serotina in vitro Cultures. Hortscience, 41(1): 193-201.
  • Gurel, S., Gulsen, Y. (1998). The Effects of IBA and BAP on in vitro Shoot Production of Almond (Amygdalus communis L.).Tr. J. of Botany, 22: 375-379.
  • Hatipoglu, R. (1999). Bitki Biyoteknolojisi. Çukurova Üniv. Zir. Fak. Genel Yayın No:190, Ders Kitabı Yayın No: A-58, Adana,176s.
  • Iqbal, N., Khan, N. A., Ferrante, A., Trivellini, A., Francini, A., Khan, M.I.R. (2017). Ethylene role in plant growth, development and senescence: interaction with other phytohormones. Frontiers in Plant Science, v.8, p.475, 2017. DOI: https://doi.org/10.3389/fpls.2017.00475
  • Jain, N., Babbar, S.B. (2003). Regeneration of “Juvenile” Plants of Black Plum, Syzygium cuminii Skeels, From Nodal Exp of Mature Trees. Plant Cell, Tissue and Organ Culture, 73: 257-263.
  • Kozai, T., Kubota, C., Jeong, B.R. (1997). Environmental Control For The Large-ScaleProduction Of Plants Through In Vitro Techniques. Plant Cell Tiss. Organ Culture, 51: 49-56.
  • Mansuroglu, S., Gurel, E. (2001). Bitki Biyoteknolojisi-I Doku Kültürü Ve Uygulamaları. Selçuk Üniversitesi Basımevi, Konya, 281s.
  • Muna, A.S., Ahmad, A.K., Mahmoud , K., Abdulrahman, K. (1999). In Vitro Propagation Of A Semi-Drawting Cherry Rootstock. Plant Cell and Tissue And Organ Culture, 59: 203- 208.
  • Nesom, G.L. (2014). Citrus trifoliata (Rutaceae): Review of biology and distribution in the USA. Phytoneuron 2014-46: 1–14. Published 1 May 2014. ISSN 2153 733X
  • Nikolić T. (2019): Flora Croatica – vaskularna flora Republike Hrvatske, Volumen 4. Ekskurzijska flora. Alfa d.d., Zagreb, v-x, 3-664.
  • Pruski, K., Astatkie, T., Nowak, J. (2005). Tissue Culture Propagation of Mongolian Cherry (Prunus fruticosa) and Nanking Cherry (Prunus tomentosa). Plant Cell, Tissue and Organ Culture, 82: 207-211.
  • Simsek, O. , Donmez, D., Aka Kaçar, Y. (2018). Bazı Turunçgil Anaçlarının In vitro Kuraklık Stresi Koşullarında Performanslarının Araştırılması. Yuzuncu Yıl University Journal of Agricultural Sciences, 28 (3) , 305-310 . DOI: 10.29133/yyutbd
  • Walsh, C.S. (2003). Plant Hormones. Concise Encyclopedia of Temperate Tree Fruit, Edited by Baugher T.A. and Singha, 245-250, ISBN 1560229411, Haworth Press.
Year 2022, Volume: 6 Issue: 4, 598 - 604, 30.12.2022
https://doi.org/10.31015/jaefs.2022.4.13

Abstract

Project Number

21096

References

  • Ainsley, P.J., Collins, G.G., Sedgley, M. (2001a). In Vitro Rooting of Almond (Prunus dulcis Mill.). In vitro Cellular and Development Biology Plant, 37:778-785.
  • Ainsley, P.J., Hammerschlag, F.A., Bertozzi, T., Collins, G.G., Sedgley, M. (2001b). Regeneration of Almond from Immature Seed Cotyledons. Plant Cell, Tissue and Organ Culture, 67: 221-226.
  • Ali, S., Mirza, B. (2006). Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explanttype and hormone concentration. Acta Botanica Croatica 65(2), 137-146.
  • Ak, B.E., Karahan Kiyar, P., Hatipoglu, I.H., Dikmetas, B. (2021). Effects of Different BA and IBA Concentrations on Proliferation and Rooting of ‘Garnem’ Rootstock In-vitro Propagation. International Journal of Agriculture Environment and Food Sciences, 5(4):470-476, DOI: 10.31015/jaefs.2021.4.6
  • Amgai, R.B., Prasai, H.K., Pandey, Y.R. (2016). Hormonal effect on mandarin orange (Citrusreticulata Blanco) micropropagation. Nepal Journal of Biotechnology, 4(1), 33-36. doi: 10.3126/njb.v4i1.16344
  • Babaoglu, M., Gurel, E. Ozcan, S. (2002). Bitki Biyoteknolojisi. Selçuk Üniversitesi Vakfı Yayınları, 374s.
  • Cengiz, M., Aka Kacar, Y. (2019). Micropropagation of Some Citrus Rootstocks with Classical and New Generation Tissue Culture Techniques. Turkish Journal of Agriculture - Food Science and Technology 7(9):1469.
  • Chamandoosti (2017).International Journal of Environmental&AgricultureResearch (IJOEAR).2454-1850
  • Espinosa, A.C., Pijut, P.M., Michler, C.H. (2006). Adventitious Shoot Regeneration and Rooting of Prunus serotina in vitro Cultures. Hortscience, 41(1): 193-201.
  • Gurel, S., Gulsen, Y. (1998). The Effects of IBA and BAP on in vitro Shoot Production of Almond (Amygdalus communis L.).Tr. J. of Botany, 22: 375-379.
  • Hatipoglu, R. (1999). Bitki Biyoteknolojisi. Çukurova Üniv. Zir. Fak. Genel Yayın No:190, Ders Kitabı Yayın No: A-58, Adana,176s.
  • Iqbal, N., Khan, N. A., Ferrante, A., Trivellini, A., Francini, A., Khan, M.I.R. (2017). Ethylene role in plant growth, development and senescence: interaction with other phytohormones. Frontiers in Plant Science, v.8, p.475, 2017. DOI: https://doi.org/10.3389/fpls.2017.00475
  • Jain, N., Babbar, S.B. (2003). Regeneration of “Juvenile” Plants of Black Plum, Syzygium cuminii Skeels, From Nodal Exp of Mature Trees. Plant Cell, Tissue and Organ Culture, 73: 257-263.
  • Kozai, T., Kubota, C., Jeong, B.R. (1997). Environmental Control For The Large-ScaleProduction Of Plants Through In Vitro Techniques. Plant Cell Tiss. Organ Culture, 51: 49-56.
  • Mansuroglu, S., Gurel, E. (2001). Bitki Biyoteknolojisi-I Doku Kültürü Ve Uygulamaları. Selçuk Üniversitesi Basımevi, Konya, 281s.
  • Muna, A.S., Ahmad, A.K., Mahmoud , K., Abdulrahman, K. (1999). In Vitro Propagation Of A Semi-Drawting Cherry Rootstock. Plant Cell and Tissue And Organ Culture, 59: 203- 208.
  • Nesom, G.L. (2014). Citrus trifoliata (Rutaceae): Review of biology and distribution in the USA. Phytoneuron 2014-46: 1–14. Published 1 May 2014. ISSN 2153 733X
  • Nikolić T. (2019): Flora Croatica – vaskularna flora Republike Hrvatske, Volumen 4. Ekskurzijska flora. Alfa d.d., Zagreb, v-x, 3-664.
  • Pruski, K., Astatkie, T., Nowak, J. (2005). Tissue Culture Propagation of Mongolian Cherry (Prunus fruticosa) and Nanking Cherry (Prunus tomentosa). Plant Cell, Tissue and Organ Culture, 82: 207-211.
  • Simsek, O. , Donmez, D., Aka Kaçar, Y. (2018). Bazı Turunçgil Anaçlarının In vitro Kuraklık Stresi Koşullarında Performanslarının Araştırılması. Yuzuncu Yıl University Journal of Agricultural Sciences, 28 (3) , 305-310 . DOI: 10.29133/yyutbd
  • Walsh, C.S. (2003). Plant Hormones. Concise Encyclopedia of Temperate Tree Fruit, Edited by Baugher T.A. and Singha, 245-250, ISBN 1560229411, Haworth Press.
There are 21 citations in total.

Details

Primary Language English
Subjects Botany, Horticultural Production
Journal Section Research Articles
Authors

Elif Subasi Sevinc 0000-0001-5300-2534

Bekir Erol Ak 0000-0001-6938-942X

Ibrahim Halil Hatipoglu 0000-0002-7236-4976

Heydem Ekinci 0000-0002-1828-7367

Project Number 21096
Publication Date December 30, 2022
Submission Date September 25, 2022
Acceptance Date October 27, 2022
Published in Issue Year 2022 Volume: 6 Issue: 4

Cite

APA Subasi Sevinc, E., Ak, B. E., Hatipoglu, I. H., Ekinci, H. (2022). Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.). International Journal of Agriculture Environment and Food Sciences, 6(4), 598-604. https://doi.org/10.31015/jaefs.2022.4.13


The International Journal of Agriculture, Environment and Food Sciences content is licensed under a Creative Commons Attribution-NonCommercial (CC BY-NC) 4.0 International License which permits third parties to share and adapt the content for non-commercial purposes by giving the appropriate credit to the original work. Authors retain the copyright of their published work in the International Journal of Agriculture, Environment and Food Sciences. 

Web:  dergipark.org.tr/jaefs  E-mail: editor@jaefs.com WhatsApp: +90 850 309 59 27