Artabel Gölleri (Gümüşhane) Sedimentlerinden İzole Edilen Aktinobakterilerin Antimikrobiyal Madde ve Endüstriyel Önemi Olan Enzimleri Üretme Kapasitelerinin Belirlenmesi

Kadriye Özcan

doi:10.35229/jaes.561017

TR EN

Artabel Gölleri (Gümüşhane) Sedimentlerinden İzole Edilen Aktinobakterilerin Antimikrobiyal Madde ve Endüstriyel Önemi Olan Enzimleri Üretme Kapasitelerinin Belirlenmesi

Öz

Determination of Capacity of Actinobacteria Isolated From Artabel Lakes (Gümüşhane) Sediment to Produce Antimicrobial Agents and Enzymes of Industrial Importance

Abstract

Bu çalışmada, Artabel göllerinden alınan sediment örneklerinden aktinobakteri izolasyonu gerçekleştirilmiş ve izolatların antimikrobiyal ve enzim üretme kapasiteleri araştırılmıştır. 5 farklı göl sedimentinden SCA, R2A, M6, SM3, AİA ve M1 besiyerileri kullanılarak toplamda 48 izolat elde edilmiştir. İzolatların, çapraz çizgi ekim ile antimikrobiyal aktivitesi, uygun besiyeriler kullanılarak hazırlanan petrilerde ise lipaz, amilaz, proteaz, pektinaz ve selülaz üzerine enzim üretim kapasiteleri belirlenmiştir. Tarama sonucunda, izolatların %79,2’i antimikrobiyal aktivite, %85,4’ü ise enzim aktivitesi sergilerken %8,3’ünde herhangi bir aktiviteye rastlanmamıştır. Antimikrobiyal aktivite yoğun olarak C. albicans (%64,6) üzerine gerçekleşirken, S. thyphymurium’a karşı aktivite tespit edilmemiştir. İzolatlar en fazla amilaz enzimini üretme eğilimine sahip bulunmuştur. Besiyeri ve aktif izolatlar arasındaki bağlantı incelendiğinde, SCA besiyeri en fazla aktinobakterinin izole edildiği besiyeri olurken C. albicans inhibisyonu yüksek olan izolatların elde edilmesini de sağlamıştır. Bu çalışmada, M6 ve SCA enzim üretimi, M1 ise antimikrobiyal aktivite taramalarında aktif izolatların elde edilmesi bakımından en etkili besiyeriler olarak tespit edilmiştir. Sonuç olarak Artabel göllerinden elde edilen aktinobakterilerin geniş spektrumda antimikrobiyal ve enzim üretim kapasitesine sahip olduğu bulunmuştur. Bu özellikleri nedeniyle endüstriyel veya farmakolojik öneme sahip bileşiklerin eldesinde kaynak olarak kullanılma potansiyeline sahip oldukları düşünülmektedir.

Keywords

Aktinobakteri,Antimikrobiyal aktivite,Artabel,Enzim üretim kapasitesi

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Kadriye Özcan ^*
0000-0002-4913-6035
Türkiye

Publication Date

August 31, 2019

Submission Date

May 6, 2019

Acceptance Date

June 13, 2019

Published in Issue

Year 2019 Volume: 4 Number: 2

DOI

https://doi.org/10.35229/jaes.561017

IZ

https://izlik.org/JA57TJ69NE

APA

Özcan, K. (2019). Artabel Gölleri (Gümüşhane) Sedimentlerinden İzole Edilen Aktinobakterilerin Antimikrobiyal Madde ve Endüstriyel Önemi Olan Enzimleri Üretme Kapasitelerinin Belirlenmesi. Journal of Anatolian Environmental and Animal Sciences, 4(2), 166-173. https://doi.org/10.35229/jaes.561017

Cited By

Endemik Onosma nana DC.’nin Kimyasal Bileşimi, Antimikrobiyal, Antioksidan ve Enzim İnhibisyon Aktivitesinin Araştırılması

Karadeniz Fen Bilimleri Dergisi

https://doi.org/10.31466/kfbd.1472704

EBSCOHost Scilit CABI

JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS