Artabel Gölleri (Gümüşhane) Sedimentlerinden İzole Edilen Aktinobakterilerin Antimikrobiyal Madde ve Endüstriyel Önemi Olan Enzimleri Üretme Kapasitelerinin Belirlenmesi

Yıl 2019, Cilt: 4 Sayı: 2, 166 - 173, 31.08.2019

Kadriye Özcan

https://doi.org/10.35229/jaes.561017

Cited By: 1

Öz

Determination of Capacity of Actinobacteria Isolated From Artabel Lakes (Gümüşhane) Sediment to Produce Antimicrobial Agents and Enzymes of Industrial Importance

Öz

Bu çalışmada,
Artabel göllerinden alınan sediment örneklerinden aktinobakteri izolasyonu
gerçekleştirilmiş ve izolatların antimikrobiyal ve enzim üretme kapasiteleri
araştırılmıştır. 5 farklı göl sedimentinden SCA, R2A, M6, SM3, AİA ve M1
besiyerileri kullanılarak toplamda 48 izolat elde edilmiştir. İzolatların, çapraz
çizgi ekim ile antimikrobiyal aktivitesi, uygun besiyeriler kullanılarak
hazırlanan petrilerde ise lipaz, amilaz, proteaz, pektinaz ve selülaz üzerine
enzim üretim kapasiteleri belirlenmiştir. Tarama sonucunda, izolatların %79,2’i
antimikrobiyal aktivite, %85,4’ü ise enzim aktivitesi sergilerken %8,3’ünde
herhangi bir aktiviteye rastlanmamıştır. Antimikrobiyal aktivite yoğun olarak C. albicans (%64,6) üzerine
gerçekleşirken, S. thyphymurium’a
karşı aktivite tespit edilmemiştir. İzolatlar en fazla amilaz enzimini üretme
eğilimine sahip bulunmuştur. Besiyeri ve aktif izolatlar arasındaki bağlantı
incelendiğinde, SCA besiyeri en fazla aktinobakterinin izole edildiği besiyeri
olurken C. albicans inhibisyonu
yüksek olan izolatların elde edilmesini de sağlamıştır. Bu çalışmada, M6 ve SCA
enzim üretimi, M1 ise antimikrobiyal aktivite taramalarında aktif izolatların
elde edilmesi bakımından en etkili besiyeriler olarak tespit edilmiştir. Sonuç
olarak Artabel göllerinden elde edilen aktinobakterilerin geniş spektrumda
antimikrobiyal ve enzim üretim kapasitesine sahip olduğu bulunmuştur. Bu
özellikleri nedeniyle endüstriyel veya farmakolojik öneme sahip bileşiklerin
eldesinde kaynak olarak kullanılma potansiyeline sahip oldukları
düşünülmektedir.

Anahtar Kelimeler

Aktinobakteri, Antimikrobiyal aktivite, Artabel, Enzim üretim kapasitesi

Kaynakça

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